Yes, this is how to validate a spectrophotometer for photometric accuracy using our potassium dichromate filter. If you have any questions, please contact us at info@fireflysci.com -FireflySci
The idea of using perchloric acid as a blank comes from the scientific observation that in order for a spectrophotometer to accurately identify a given concentration of potassium dichromate in a solvent (and perchloric acid is used as solvent), the photometric absorbance of a solvent needs to be subtracted from the photometric absorbance of a solution which has both ingredients in it: potassium dichromate + perchloric acid.
Hi Shubham, this is a little outside of our wheelhouse but I'd check online for some papers that deal with this. Try this one: www.ceacsu.edu.pk/PDF%20file/Volume%2014%20No%201/01-15-PJAEC-26032013-008.pdf
Hi! The best place to check is in the manual for your machine. The most common term used is to "zero" the machine but I've heard it called blanking by many people.
@@fireflysci1194 Actully sir.... it has only four button named as mode, Print, T 0% and absorbance 100 and a knob foe wavelength setting...... and there is a black colour cutteve in the box named as %transmittance....
Hi Sivako, We are showing how to use an already prepared potassium dichromate solution to calibrate a spectrophotometer. We actually no longer produce these standards any longer. I would suggest reading the following article for guidance: www.quora.com/How-do-I-prepare-a-0-1N-potassium-dichromate-solution#:~:text=Here%20oxidation%20state%20changed%20from,it%20in%20one%20litre%20solution. All the best, FireflySci
Hi Farhad, please check all of our videos that show how to validate for photometric accuracy, wavelength accuracy and stray light. Which function do you need to do?
Hi, so i shouldnt use DI water as a blank? I have the potassium dichromate of diff concs in 0.005 M sulfuric acid but i need perchlroic acid as blank? Sulfuric acid didnt work for my machine..
Hi Swagotom. There can be a number of issues if your machine does not fall within tolerance. Your first step should be to contact the manufacturer and explain your problem to them. They will be able to help you get your machine up and working again.
please help me.... I am pursuing my thesis work on Antioxidant properties by DPPH assay, I'm confused about the measurement of the absorbance at 517 nm in a UV spectrophotometer. my aim is to observe the decoloration of DPPH by an antioxidant like as plant extract. i have to use the different concentration of extracts(ex. 400 ug/ml, 200ug/ml, 100ug/ml, 50ug/ml) sample then what should be in the reference cell and what in the sample cell, while using different concentration and solvent is Methanol?
Hi Sajal, if you are measuring at 517nm your best bet would be to validate your machine around this wavelength point. You can save up front and recalibration cost by going with our HF-Series standards found here, which can validate your machine from 400-700nm: www.fireflysci.com/photometric-accuracy/hf-series-vis-photometric-accuracy-calibration-standards Thanks for your question! FireflySci
Is it right method to calibrate spectrophotometer???
Yes, this is how to validate a spectrophotometer for photometric accuracy using our potassium dichromate filter. If you have any questions, please contact us at info@fireflysci.com -FireflySci
What's the logic behind using perchloric acid and potassium dichromate?
The idea of using perchloric acid as a blank comes from the scientific observation that in order for a spectrophotometer to accurately identify a given concentration of potassium dichromate in a solvent (and perchloric acid is used as solvent), the photometric absorbance of a solvent needs to be subtracted from the photometric absorbance of a solution which has both ingredients in it: potassium dichromate + perchloric acid.
thanks so Mach
You're welcome, Ahmad!
how to determine zinc on spectrophotometer
Hi Shubham, this is a little outside of our wheelhouse but I'd check online for some papers that deal with this. Try this one: www.ceacsu.edu.pk/PDF%20file/Volume%2014%20No%201/01-15-PJAEC-26032013-008.pdf
Sir... i have a spectrophoto meter .. there is no knob to make the blank reading zero.. how to use it...
Hi! The best place to check is in the manual for your machine. The most common term used is to "zero" the machine but I've heard it called blanking by many people.
@@fireflysci1194 Actully sir.... it has only four button named as mode, Print, T 0% and absorbance 100 and a knob foe wavelength setting......
and there is a black colour cutteve in the box named as %transmittance....
Thanks. It's very informative video
How to prepare K2Cr2O7 you are showing absorbance directly
Hi Sivako,
We are showing how to use an already prepared potassium dichromate solution to calibrate a spectrophotometer. We actually no longer produce these standards any longer. I would suggest reading the following article for guidance: www.quora.com/How-do-I-prepare-a-0-1N-potassium-dichromate-solution#:~:text=Here%20oxidation%20state%20changed%20from,it%20in%20one%20litre%20solution.
All the best,
FireflySci
Hi I m Farhad my spectrophotometer hi need to calibration 601shimad
Hi Farhad, please check all of our videos that show how to validate for photometric accuracy, wavelength accuracy and stray light. Which function do you need to do?
Hi, so i shouldnt use DI water as a blank? I have the potassium dichromate of diff concs in 0.005 M sulfuric acid but i need perchlroic acid as blank? Sulfuric acid didnt work for my machine..
You must use the blank that comes with your kit. The perchloric blanks we provide are perfectly balanced with our filters.
What you have used as blank??
We provide a blank with each potassium dichromate filter we manufacture.
Very useful video, thx
We're happy to help! -FireflySci
Nice video!
Thanks!
Thanks
Thanks :).
How, what if your spectrophotometer does not give the values it should give?
Hi Swagotom. There can be a number of issues if your machine does not fall within tolerance. Your first step should be to contact the manufacturer and explain your problem to them. They will be able to help you get your machine up and working again.
how to determine rare earth elements on UV-Vis
please help me....
I am pursuing my thesis work on Antioxidant properties by DPPH assay, I'm confused about the measurement of the absorbance at 517 nm in a UV spectrophotometer.
my aim is to observe the decoloration of DPPH by an antioxidant like as plant extract. i have to use the different concentration of extracts(ex. 400 ug/ml, 200ug/ml, 100ug/ml, 50ug/ml) sample then what should be in the reference cell and what in the sample cell, while using different concentration and solvent is Methanol?
Hi Sajal, if you are measuring at 517nm your best bet would be to validate your machine around this wavelength point. You can save up front and recalibration cost by going with our HF-Series standards found here, which can validate your machine from 400-700nm: www.fireflysci.com/photometric-accuracy/hf-series-vis-photometric-accuracy-calibration-standards
Thanks for your question!
FireflySci
Thank you sir
We hope this video helps! Thanks -FireflySci
Thanks for education,i'm from Indonesia.
We're grateful we can be of service! Thanks- FireflySci
Sorry, I think this is a verification of the equipment, no a calibration
we need master solution