DIY gas chromatograph
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- เผยแพร่เมื่อ 4 พ.ค. 2019
- A brief introduction to a hobby project: a homebrew gas chromatograph!
If there's interest in the project I'll make additional videos dedicated to the design and manufacture of the components of the instrument.
This is my first attempt at video'ing so any comments on the format is appreciated.
Keep in mind there are certain inherent dangers that comes with handling compressed gasses and this kind of scientific instruments! If you feel like doing a similar project keep this in mind, and note that this video is NOT intended to explain these. - วิทยาศาสตร์และเทคโนโลยี
Wow! That looks great! Yeah, I'm really interested to see what you do next.
Phenomenal you've inspired me my brother. Who needs money when we have brains.
Get in touch with Ben at the channel Applied Science. He just home built a Mass Spectrometer and briefly mentioned interest in building a chromatograph. If nothing else I think the 2 of you would probably be able to help each other out on your projects.
Great project, please keep us updated!
Very inspirational project, amazing. Good job!
I was literally just thinking of something like this. Only with a miniature solvent bottle to make it super space efficient for casual home lab use. Will deff be looking forward to more videos on this.
Me too!
Rik DeBacco yeah, throw one into a pelican case and take it into the field
Wow! What a cool project.
Very awesome! More details are always good when come to making something. The acts and the actors later are what can make the best appear to be perceived as not good. Thanks for sharing! Looking forward to watching more of your videos.
Beautiful work.
Pls continue with this great project, thank you in advance
Dude. You're brilliant
this is absolutely amazing
This is great, I love it! Thank you!
Oh man, I’m trying to do this with liquid chromatography. I’m so happy I found this.
Stick around! I just got an old HPLC system and I'm going to make some vids about DIY HPLC detectors and columns for it soon!
@@chromatogiraffery3104 Neat, what kind? I have a HP 1050 that I got for very cheap in non-working condition and repaired. I would say it's definitely harder to DIY a HPLC than a GC.
@@DavidRobertsonUK The old Waters 600 workhorse, and a DAD. It's probably not feasible to DIY the pumping elements and such, but detectors definitely are!
@@chromatogiraffery3104 Yeah I agree re detectors. I just have a variable wavelength detector which honestly is perfectly fine for me. Actually, I have two, but one needs a new deuterium lamp. If it didn't need that then I'd hook them up in series to have the option of using two at different wavelength settings. But that doesn't offer enough value to me at the moment to justify buying a new lamp (they're pretty expensive).
Thank you very much ! very inspirational !
Wooooww, súper genial amigo... Ya quiero ver cómo lo hicistes... Saludos desde Jalisco
Wow! This is cool.
this is excellent, do you have any plans/schematics or any sites with the latter which can help me recreate your project here?
more specifically details on the element and thermal detector build
Pretty good Whetstone bridge ! I was familiar with gas chromatographs, mass spectrometer, atomic absorption photometer, I used to worked in gasoline refinery, I didn't pay attention to it until I saw this!, That time we could use only mV paper plotter for the result, Sometime the brands of intruments stuck to my memory, Perkin Elmers sounds familiar?
Any updates on the project? It is such a cool endeavor, I hope you can find the time to make another video.
Wish you all the best and keep up the great work!
Very neat. I have an old HPLC in my garage, and I'm very tempted to try to DIY a GC because it seems like it wouldn't be too difficult to do.
I have some questions:
1. Why are there four sensor elements in the thermal conductivity detector? it seems to me that only two are needed
2. How are these sensor elements constructed? I assume it's something like a tungsten filament from a small lamp, but how is this sealed into the T fitting?
@@DavidRobertsonUK I'm working on a TCD detail followup, just need to re-discover my documentation! but in brief:
1. only two is needed, but then two resistors are needed that needs to be matched accurately, and be in thermal equilibrium. with 4 elements this happens naturally, so in that sense it's easier to zero.
2. Right on the money! I found cylindrical 1/8" bulbs that fits naturally in the T fittings (with PTFE ferrules)
@@chromatogiraffery3104 Okay, thanks!
Love it I will like it to put it to a test
Cool project! Using Helium is maybe needed for your kind of stable phase but it is also the most expensive way to do! Try Argon 5.0 as mobile phase. It's much cheaper than He. I'd like to buil one.
Very interesting! Another (better start) way to do this is to buy a small chromatograph from ebay (GOW-MAC) units that regularly come up for around $150 with everything there. These are very basic instruments that are used for fixed setup in a production environment where sample after sample is run. However, the instrument has all the stuff you need already connected up to make quite a nice amateur machine. You can use commercial columns or homemade columns of course. I will do a video on mine when I get it finished restoring.
I considered those, but I thought it was a nice challenge to use a minimum of scientific surplus parts. And since the gow-mac units go all the way back to the 1960's I was a bit worried about asbestos...
It doesn't have asbestos. It's all glass fiber.
@@glasslinger That's good to know! but at the time I couldn't verify that, given the expensive shipping and all that.
Any status on this?
thankyou
Hello I believe this project is really amazing.. I love this.. could you provide more details about building something like this? I would be very grateful
Did you pack the column before or after shaping it? Any suggestions on techniques/tools to help pack a column?
Hi there very interesting. I am working on a mass spectrometer (see video), and this is something I would like to look at in the future. Very interested to see more. Well done.
Thanks! Your MS video is very interesting! I am also working on one, TOF with an electron multiplier as ion source. If you use a gc front end for a MS I would recommend a capilæsry column, As a packed column would let too much gas flow and compound into the vacuum system
@@chromatogiraffery3104 Yes I have seen that video before you mentioned it - interesting, I had not associated with your gc project. I have used 12V 20W tungsten lamps for electron sources. What is the advantage of using electron multiplier as electron source? I avoided using a multiplier as ion detector because they sound a bit delicate and expensive on ebay. I have home made amp with LMC662 and 12G Ohms and careful 3D layout noise is about 10fA and response time about 1ms.
Getting the sample in is not problem, I have home made leak valve using tiny sewing needle in solder seating, two stages, affluent from first stage pumped away by aux pump. TOF sounds interesting and also magnetic sector with double focus sounds interesting, but its finding the time. Good to talk to someone with similar interests.
@@reytdhfuytf Using electron multipliers allows one to use pulse counting techniques like with geiger counters and scintillation detectors, which has some advantages. Especially for timing, one can get fast precise timing pulses for time-of-flight spectroscopy.
In my opinion the main advantage is to avoid dealing with all the very high impedance electrometer stuff!
Did you build your quadrupole yourself or is it re-purposed?
@@chromatogiraffery3104 I know why electron multipliers are used as ion detectors, although I decided not to use one and accept the poorer performance. My question is why did you decide to use a light source and electron multiplier as an electron source? I did make the quadrupole myself, it uses standard round bar stock, I chose bits without any defects, but the surface accuracy is a concern.
Great job man... Im looking to make one too. Looking for inspiration on TH-cam
good job... one questions. how is live time of your filament? i made similar solution and after 5 hour work "0" point beginning of "swim"
Brilliantly Simple ! Thanks for sharing your projects. Pardon my ignorance, what kind of detectors are those 4 detectors (2 before entering the oven loop and those 2 after leaving the oven) ?
Thanks! A thermal conductivity detector! I have another vid with details
@@chromatogiraffery3104 Thank you! This is indeed a very interesting project from a type of experiment no one does apparently... i do like the ardino incorporation. What type of Analaog to digital converter ic do you use? i am always struggeling to find good A/D Converters for arduino usage 🙂
How did you make the packed column btw? I used bentonite powder before to make plugs and if you shove it into a tube it tends to make this really solid plug. Is the packing in the tube really tight and smashed in there? Or is it more like how you would dry-load a silica column and let pressure do the work?
Thanks for the interest! The columns was packed simply by plugging one end with glass wool, applying vacuum on that end and a funnel on the other, and then tapping the tube until it wouldn't take more. I found that particle size was critical, and all adsorbents were sieved through an 80 and 100 mesh test sieve to yield only particles in that range. around 0.2mm dia. Bentonite clay is very tacky when damp. maybe it needs to be oven dried before but I didn't have any problems.
This is awesome. If you have any questions or run into problems please feel free to contact me. I look forward to seeing what you do next.
Excellent work!! i have a question, what is the method you should use to confer certain affinity to your column? for example, if i want to confer olefinic affinity what should i do?
Thanks! Not sure, but as always in GC you can compare retention times of different but similar compounds.
Do the tcd sensors just detect the drop in temperature as the gas passes over it? If so, why cannot this detection be done with a single tungsten filament just detecting fluctuations in temperature?
If you do need two sensors, ould you do this with one set of detectors using the carrier gas before injection as the baseline and then do the comparison via software?
You absolutely could, but the idea with measuring a differential signal is that fluctuations in filament voltage/current, and carrier flow rate are canceled out. This gives a nicer baseline. Agilent TCD's has a single filament that's rapidly switched between pure carrier and column efluent to compare the two.
Круто. Я захоплений. Я також мріяв про це, щоб зробити. Я хотів визначати метиловий спирт в етиловому. І багато іних речей. Я радий це бачити. Цікаво було б детальніше дізнатися про детектор.
Thank you! More about the detector here: th-cam.com/video/OqsQVFmSf-A/w-d-xo.html
Can this be used to measure CH4/CO2/H2S content in biogas, and CH4/O2 in engine exhaust?
I am not a specialist but i say no.....problem is that exhaust gas is pretty dirty and wet together! First of all you need to get rid of the PM and Humidtity/Water etc. Particles and a lot of different gases are in it and you will never find a ONE in all column for that.
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Hello, its excellent. What software you use for share the chromatographic peaks?
PLX-DAC and an excel macro for plotting it real time. Not very elegant but it works
@@chromatogiraffery3104 How can I contact you for more information? I Will send you some pic about my project, I need your help about electronics.
What is your carrier flow rate?
Thanks for the interest! Flow rate is 17.3 ml/min at 25 PSI inlet pressure, which is about standard for a 3 ft 1/8" column packed with 80-100 mesh solid support.
We need more instructions here.
Hi, do you sell these? i cant find your email address. Can you please provide me one.
Unfortunately, I dont sell these, I just built the one!
exact same as done by an german kid 4months before,