Hybridoma Technology-Production and Purification-Biotechnology-Unit 3- B. Pharmacy 6 Sem--Lect.9
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- เผยแพร่เมื่อ 13 ต.ค. 2024
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Hybridoma means the hybridization of activated B lymphocytes and myeloma cells in vitro. The production of monoclonal antibodies by the hybrid cells is referred to as hybridoma technology.
Monoclonal antibody is a single type of antibody that is directed against a specific antigen is determinant or epitope.
Production of monoclonal antibodies for steps in hybridoma technology.
1) Immunization
2) Cell fusion
3) Selection of hybridomas
4) Screening the product
5) Cloning and propagation
6) Characterization and storage
Application of monoclonal antibodies
Monoclonal antibodies with specificity and high-purity have a wide range of applications
1) Diagnostic application
2) Therapeutic application
3) Protein purification and
4) Miscellaneous application
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