Beautiful example of an extraction! A fluorescent compound in the organic phase and copper ions in the aqueous one, beautiful! But I don't drink tea or coffee, i drink Redbull and do my extractions in the lab only^^ Some additional information to your explanation: Polarity is most important beside density. Your synthesis product may be very polar and go into the aqueous layer. You can use that, for example with amines, the free base usually is non polar and dissolves in the organic phase, while it's HCl salt after acidification with HCl is a polar salt, so in dissolves in the aqueous layer. Very useful for cleanup. Furthermore, density, usually chlorinated solvents build the lower phase with water, hydrocarbons and hydroxycarbons the upper one. But that isn't always the case, heavy salts can make the water layer heavier than dichloromethane for example, heavy organic molecules can make the organic layer heavier than water etc. Always watch which phase goes were while pouring into the separatory funnel in order to not lose your product. Usually you should always shake a separatory funnel with the stop cock end direction fume hood, while shaking the other end with the other hand, pointed at your hip. Usually gas will get liberated, so first swirl it open, then start shaking and venting. Best practice is to hold the stop cock with the left hand und the separatory funnal and stopper with the right hand. Always do three extractions, since non miscible solvents are still partly soluble one in another. Then combine your phases containing your product. If it's in the aqueous one, boil off the organic solvent with heat and maybe vacuum. If, as more common, it's in the organic one, you may neutralize it with a saturated solution of sodium bicarbonate. Then dry it with saturated sodium chloride, which takes up water due to osmotic effects. Then, as mentioned, dry completely using anhydrous sodium or magnesium sulfate. If it's a nice powder flowing up, it's dry, if it clumps up, it's saturated with water, so you'll have to add more. When filtering it off, always rinse with a bit of fresh solvent, or else a lot of your product will stay in the filter with the sulfate salt. For professionals using rotavaps: Yes it says to evaporate ether or DCM at atmospheric pressure, but that doesn't mean to not turn on the vacuum pump. It's a closed system and you're evaporating stuff, so just set it at 900 mbar to keep it from blowing up. Since vacuum has instant effect, but heat has to be conducted, always first set the correct vacuum for your solvent according to the 60/40/20 °C table (Waterbath at 60 °C, boiling point at 40 °C and cooling water at 20 °C. Gives best and fastest evaporation. If correctly used) and then put the flask into the water bath. Otherwise it'll boil over instantly. Rotation is necessary to avoid boiling delay, but rotating to fast can hinder evaporation. When almost dry, take out the flask of the water bath, vent to atmospheric pressure, empty the receiving flask, and apply high vacuum to dry your product if it itself isn't volatile.
Hi Please help in DEHP EXTRACTION METHOD Using ethanol with Uv spectroscopy Or other method to determine exact value DEHP(CAS Number: 117-81-7)in extraction solvent
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ASMR starting from 5:39, loved the video
Best video ❤️ it's like I was in a dream for a moment
Of course
Please consider providing references to transformations you mention in the video so that the viewer can be better informed on the topic of the video.
Huge appreciation ❤❤❤
Beautiful example of an extraction! A fluorescent compound in the organic phase and copper ions in the aqueous one, beautiful! But I don't drink tea or coffee, i drink Redbull and do my extractions in the lab only^^
Some additional information to your explanation:
Polarity is most important beside density. Your synthesis product may be very polar and go into the aqueous layer. You can use that, for example with amines, the free base usually is non polar and dissolves in the organic phase, while it's HCl salt after acidification with HCl is a polar salt, so in dissolves in the aqueous layer. Very useful for cleanup. Furthermore, density, usually chlorinated solvents build the lower phase with water, hydrocarbons and hydroxycarbons the upper one. But that isn't always the case, heavy salts can make the water layer heavier than dichloromethane for example, heavy organic molecules can make the organic layer heavier than water etc. Always watch which phase goes were while pouring into the separatory funnel in order to not lose your product.
Usually you should always shake a separatory funnel with the stop cock end direction fume hood, while shaking the other end with the other hand, pointed at your hip. Usually gas will get liberated, so first swirl it open, then start shaking and venting. Best practice is to hold the stop cock with the left hand und the separatory funnal and stopper with the right hand. Always do three extractions, since non miscible solvents are still partly soluble one in another. Then combine your phases containing your product. If it's in the aqueous one, boil off the organic solvent with heat and maybe vacuum. If, as more common, it's in the organic one, you may neutralize it with a saturated solution of sodium bicarbonate. Then dry it with saturated sodium chloride, which takes up water due to osmotic effects. Then, as mentioned, dry completely using anhydrous sodium or magnesium sulfate. If it's a nice powder flowing up, it's dry, if it clumps up, it's saturated with water, so you'll have to add more. When filtering it off, always rinse with a bit of fresh solvent, or else a lot of your product will stay in the filter with the sulfate salt.
For professionals using rotavaps: Yes it says to evaporate ether or DCM at atmospheric pressure, but that doesn't mean to not turn on the vacuum pump. It's a closed system and you're evaporating stuff, so just set it at 900 mbar to keep it from blowing up. Since vacuum has instant effect, but heat has to be conducted, always first set the correct vacuum for your solvent according to the 60/40/20 °C table (Waterbath at 60 °C, boiling point at 40 °C and cooling water at 20 °C. Gives best and fastest evaporation. If correctly used) and then put the flask into the water bath. Otherwise it'll boil over instantly. Rotation is necessary to avoid boiling delay, but rotating to fast can hinder evaporation. When almost dry, take out the flask of the water bath, vent to atmospheric pressure, empty the receiving flask, and apply high vacuum to dry your product if it itself isn't volatile.
Wow that was very helpful. Can you please explain why we add the anhydrous magnesium sulphate
@@RadhikaSharma-oi4neit binds with water so you have a dry organic layer when u filter
Thanks ❤
Best video
Easily understandable
thank you nadia
Sonogashira coupling reaction 🥰
Hi
Please help in
DEHP EXTRACTION METHOD
Using ethanol with Uv spectroscopy
Or other method to determine exact value DEHP(CAS Number: 117-81-7)in extraction solvent
Thank you 😊
Thanks 😊
please make more videos,
Hi madam ..it vary useful
Good vid
What this squirt bottle consists of
hexane
Збс)
Thank you ❤