How to prepare plant extracts using various solvents||Methanolic extract
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- เผยแพร่เมื่อ 11 ก.ย. 2024
- Learn how to prepare various plant extracts using different solvents for biochemical assays, HPLC, GC-MS studies etc.
Do watch the biochemical assays in my other videos to know how to analyze the proteins, carbohydrates, proline, phenolics, flavonoids and many more...
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I am following your videos in my PhD work
Bro can you tell me the reference he's is using
That was so informative thank you so much. I was struggling with this to understand through paper for so long and your video cleared everything
Your video is extremely informative Sir. Thank you so much 🙏
:)
Please keep posting more videos regarding phytochemical analysis of the plant and also about essential oils of the plants.
Thanks Brother!!
Truly helpful.
Hello Sir. I have some queries about plant collection and drying processes.
1. Can we pluck leaves and leave the plucked leaves to dried separately?
2. Can we cut the roots and leaves separately and dried and powdered at the same time.
3. How long time we should dry the plant sample in shaded area?
4. After powder prepared, how long time we can use powder to carry various experiments?
I request you to please answer my doubts.
very clear explaination...👌👌🙏👍👍
Sir very good job done by you kindly share videos related to bacterial research related to metal NPs and ethyl acetate extraction of bacterial metabolites
Thank you SIR ! Much needed video.
Thank you so much. Can you share plant extract preparation in different solvent for GCMS analysis, so that I can send the sample for analysis to other lab. Thanks again.
Very much clear and informative
Thnkyou so much sir for your effort....your videos are very beneficial ......
sir , My topic of research is to determine the invitro antidiabetic properties of the medicinal plants. A humble request to you to make a video on this assay.I always watch your videos before performing assays.plz make the video as soon as possible .
Im not working in lab. Im afraid that i can't be doing any experiments for a while.
sir as you perform dpph and other assay so i told you for this bcz these videos helps me alot
Well explained Rakesh...
Thank you mam 🙏
Thank you for the clear explanation. I have a question- after methanol extraction and evaporating the methanol, when you re-dissolve the plant residue, can you use any solvent other than methanol? Can I re-dissolve the residue in DMSO to make 1 mg/mL extract in DMSO? Will it dissolve in DMSO? For my experiment I cannot use methanol. It must be DMSO that's why I am asking. Thanks.
Very informative video sir. Thank you for uploading. I have a plant whose phytoconstituents are not known. M planning for tlc bioautography can you help me how to select solvent system?
Very nice👌
Hi sir
Just need to know one thing, I have done serial plant extraction using pet ether, chcl3, and ethanol.. so will u plz tell me how can I make 1mg/ml solution of chcl3 and pet ether extract for TFC TAc, etc. Like which solvent I can use to dissolve these solvent extract for my further analysis
Thank you for the video! My project is to identify the best brewing temperature and time for green tea leaves to exhibit the most antioxidant capacity. Can I directly use the sample ( solution of tea and water) to carry out DPPH essay?
Please make a video on work mechanisms of rotary evaporator? And is there any simple method to remove solvent?
Sir wonderful explanation i have a doubt which analysis is best to test the phytochemicals in plant extract? Is GCMS is good?
Thanks a lot sir 🤝❤️
Killler
Absolutely brilliant
Thank you 🙏
It is so useful sir
Thanks 👍
Good effort, but falls short.
It was very informative... sir I have a question... after evaporation I have the extract in pasty form can I dilute it in the same way as u have shared in ur video?
Thank you for the video, sir. I was wondering what concentration of extract (in mg/mL) is needed for the DPPH assay? Thanks!
Yes sir i have the same question
very very useful video...sir will you please tell..for extraction how many solvents we can take ideally?
It depends on the work, is it Ph.D or Msc project. Ideally we take 2 polar solvents and 2 non polar solvents. You can increase if you have time and interest.
Super sir
Sir I have a doubt . I was asked to take the quantity of the sample to be dissolved in the solvent according to the concentration of the sample. Can you tell me how to check whether the sample is concentrated ? Is it by keeping the sample in the solvent i.e; 1:10 ratio overnight and if the solvent evaporates to 3/4 th it means my sample is concentrated Sir?
Thankyou, it helped me quite alot.😃
Happy its helping:)
Sir plz make more videos on phd experiment
Sir j plant extract bny h vo 20microlitre m hi hoga ky ml m nhi hoga ky
Are these extracts can be consumed by normal person?
Thank you for easy explanation of procedures.
Sir, could you please provide a reference for this procedure... ?
Thank you
Usually no paper provides or explain this as it's a well known basic procedure. Not discovered by any particular person to give reference. You can watch my video on references and download my paper and cite the same
If we do centrigation, then how we can make different concentration of extract for antibacterial activity?
Hi sir, lets say i have prepared my methanolic extract for 1mg/ml, i want to further dilute it for some range of concentrations, can i use distilled water to dilute ? this is especially for total phenolic content.
Thank you so much sir
Sir you are great.
Means a lot. Thank you :)
Sir i m doing phytoremediation of BPA.. Nd for extraction after drying i grind the sample with buffer nd aftr that i put methanol. Will it hamper in any way?
Thankyou . Its quite helpful.
Thank you brother ☺️
is there a specific number of days that medicinal plant extracts are supposed to stay before bioassay screening?
No you can test them immediately. Maximum of 1 month u can store at 0c
@@Dr.Rakesh.B thanks very much Dr. I am enjoying your videos. How about adding 1mL of 70% ethanol as a preservative and store them At 4°C ?
It will change the solubility of chemical compounds
If the plant extract is sticky and oily then is it possible to make powder??
I have got the sticky extract which is oily..after removal of methanol
Can I use it for quantitative analysis
Yes u can
@@Dr.Rakesh.B
Thank you so much sir
I need the cellulose contents in gel or viscous form , so is there any method to get gel sample?
Very informative video
Sir how many days is the macerated drug kept in beaker in methanol solvent to drying process
3-4 days
Sir plz reply how to use fresh sample for flavonoid content and phenolic content determination
Can one do vacuum (room temperature or lower) drying.
Thank you for the video sir. In my case I have to extract the chlorophyll-a from algae, so do I have to store the filter paper with solvent for 24 hours in the refrigerator before grinding it or should I have to grind and then store it in the refrigerator
Chlorophyll estimation is done by arnons method using 80% chilled acetone..Browse with this keywords you will find papers or in one video in my channel theres a video about references. Watch that video and download my paper for reference
hello, what do we need to preserve the extracts if we will use only for a few days? is anything necessary to add or no need?
How do you store powder?
I was planning to put it in a ziplock bag. Shall I store it in refrigerator or room temperature?
Ziplock will do or any air tight vials and refrigerator (freezer).
very informative bro
Hello listen please
@@T-Entertainment877 yes plz
@@mohitrai3743 bhai muje ik problem hai about methanolic extract
@@T-Entertainment877 yes bro ask
@@mohitrai3743 ap ki education kitni hai?
Gr8 video sir
Thanku so much sir, sir can u plz make video for determination of stomatal number .
Ok :)
nice video, i have a doubt, i have small amount of sample, can i use four different solvents one after another on the same sample?
@Dr.Rakesh.B SIR I need to know. If I have to synthesize nanoparticles out of ethanolic extract of powdered seeds of some plant. Do I have to completely remove ethanol from our extract or can I use it in concentrated form coz I can't evaporate it completely.?
You can make a liquid extract and use that liquid.
@@Dr.Rakesh.B liquid extract in ethanol?.
@numerasaghir2515 yes
@@Dr.Rakesh.B this will not impose some bad effect on my nanoparticles na?.
Sir how can I mail you? I want to ask about one plant extract preparation for my master’s thesis sir
Sir for our project we need to prepare the methanolic extract of aloe vera gel. Can you please guide us on how to proceed for it.
Nice
Sir how can we calculate the amount of herb needed for a certain amount of liquid extract? E.g. if we need 0.5 litre of extract for storage then how much herb will be needed for extraction?
For 1 ml of extract usually 20-25gms of dry weight is required. Its all trial and error cannot be predicted.
Is the method can be used for other sample? Means other than plant samples
Can you please tell me how much quantity of orange peel powder and ethanol should I use to make extract to find out inhibition zone of bacterial strains. Please tell.
It's all trial error I can't say just like that
@@Dr.Rakesh.B okay
Sir, Can I use this for plant protein extraction before using ultrasonication?
Nice video sir, 1f 10 mg is required for 10 ml methanol, how much methanol will be required for 0.32 mg
320mL
thank you for your clear explanation
How much percentage of ethanol is used in this process
Sir can i use the fresh plant material for methanolic extract . For eg can i homogenise the sample directly in methanol, centrifuge amd use the supernatant for DPPH assays
Yes
Bro pls explain how to make mother tincture ?
Sir I have performed extraction of plant in microwave by using methanol solvent and I have to prepare methanolic extract to detect flavonoids on tlc plate please suggest me how to do that
Sir I have one doubt, can we dissolve the powdered methanolic extract in oil
Yes but not completely. Depends on the polarity of the compounds present in extract.
Sir, can we make a nanoemulgel with w/o nanoemulsion.
Sir in hot air oven up to how much time I should keep the extract for further evaporation after rotavap n also tell plz that is dryer oven n hot air is same...
As long as you can regulate the temperature any oven is fine. Keep for 2-3 days at 40 degrees
1. Is the procedure sane for plain water extracts of plants?
2. Can these falcon tubes be used for Long term storage of extract?
Yes for both
@@Dr.Rakesh.B thank you sir
@@Dr.Rakesh.B sir wat about evaporating step? It will also be sam fr water extracts?
Yes since it's water it will take more time if you keep in room temperature. You can keep it at 40-50 degrees.
@@Dr.Rakesh.B what will happen if I skip the evaporation step?
Sir...I have a question..suppose we have no idea regarding solubility characteristic of our sample....then we have to follow successive solvent extraction method with polar to nopolar solvent or vice versa..Right? After extracting with all solvent...What will v do..we will calculate % yield for all extract or what? Before proceeding or selecting extract for any activity....pls rply soon I have to do an activity but no idea abt solubility ...which solvent should i use for preparing extract?
You have to read literature for ur paper and discuss with your supervisor
@@Dr.Rakesh.B thnx for replying sir......but no sufficient review is available for the same....
Sir, is it necessary for also vit c quantification ?
Hello Sir, I am doing an experiment on a ethanol plant extract that I bought from a well known company. I want to check its antibacterial actions against some bacterial strains. I found no literature about the preparation of the solution of the commercially available ethanol extract of the plant. Therefore, could you please guide me as how to make the solution to check the antibacterial effects of the extract? Thanks
What happens if the fresh sample is taken (eg; Fresh roots) to prepare methonolic extract? Will it affect the results?
Can you please tell me the research papers which you followed make these videos like Preparation of plant extract, DPPH, TPC
There's a separate video in my channel for references. Please Watch that
Is this same for water extraction also?
Hello sir, can i use freeze drying to evaporate the methanol after filtration?
I am Extracting oil from raw leafy vegetables without drying and powder by using soxhlet apparatus. How to prepare sample for total phenolic content?
Consider the oil directly to be ur sample and proceed. But i don't know if this will work i haven't worked on oils. Try reading papers on oil or u can ask some experienced person in research gate.
good morning sir
i want to know about plant extract for antimicrobial activities .....because this is my research topic ..plz guide me ...
Sir is it okey if i use the liquid i mean methanol in which the grind sample pourend first?
Yes
Hello Sir, I question is. For Total Flavnoid and Phenol estimation can't we use the direct extract instead of evaporating? Plz answer
U can use but the conc.wont be accurate
Thank you Sir. How will I know how much methanol will I put to my extracted plant?
sir could you please mention the article for references purpose. thank you
I have made a separate video for references pls watch it
Sir petroleum ether solvent deodorize krna hu to konse chemical used hugaa
I haven't done that no idea sorry
Hello sir I extracted my phytochemicals using n-hexane and I dried it, now can I dissolve this dried extract into some other solvents like methanol or DMSO for my further experiment
No
You should use n-hexane only
@@Dr.Rakesh.B Thank you
How to take extraction from euphorbia plant
Sir same extract is used for antimicrobial activity?
Yes you can use
Sir our guide let us make in 70 percent methanol....did u used absolute methanol means that 99.9 percent one?
Both is fine
@@Dr.Rakesh.B thank u
Sir this protocol is same for fruits samples
It is necessary to further dissole it in a methano? Cant we leave the compound undissolved in -20
You have to dissolve it in known volume of methanol so that you know the concentration. If ur experiments require crude extract in solid form then u can leave as it is and store it
@@Dr.Rakesh.B thanku sir for replying
Does Brahmi plant get extract by using hot water or cold water?
Refer from any published paper. It's not possible to know everything for everyone.
Can i use normal filter paper ?
Yes
How to contact with you sir i have query
Sir for the extraction of saussurea lappa , is methanol or ethanol is good?
Please read literatures related to it.
good evening sir
sir can we perform antibacterial activity of plant powder without any extraction if it is possible kindly guide me
i m working on a herbo mineral preparation for my dissertation work
No, you have to dissolve it in some solvent .
I dried my Methanolic, acetone and aqueous solvent at 45° in oven for 3?? 4 days. Is my sample can't be used for phenol estimation
Hello sir,may i know how to estimate the concentration of plant extract using UV double beam spectrophotometer?