Construction of cDNA library | Synthesis of cDNA | rDNA Technology | Bio science
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- เผยแพร่เมื่อ 25 ส.ค. 2024
- Construction of cDNA library | Synthesis of cDNA | rDNA Technology | Bio science
A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, of which constitute some portion of the transcriptome of the organism and are stored as a "library". cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism. Similarly, tissue-specific cDNA libraries can be produced. In eukaryotic cells the mature mRNA is already spliced, hence the cDNA produced lacks introns and can be readily expressed in a bacterial cell. While information in cDNA libraries is a powerful and useful tool since gene products are easily identified, the libraries lack information about enhancers, introns, and other regulatory elements found in a genomic DNA library.
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Recombinant Dna
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The name reverse transcriptase is due to synthasise of DNA from RNA not for 3' to 5 '
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Thankyou sir
Good explanation, but for the formation of mRNA only transcription occurs, translation is not done
And reverse transcriptase enzyme synthesizes DNA from RNA in 5' to 3' direction
Thank u for making my concept clear through this video.
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You need to clear your concept about synthesis direction movement of polymerases 👍🏻👍🏻👍🏻
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Can you please explain DNA shot gun method!!? PLEASE
Nice explanation
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As it moves to 5 prime end will form hair pin loop
Can I get the pdf of that notes , please
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Where is the link...not found in the description box
Can you help me in learning for msc zoology entrance exam?
Good explanation bro I watched this vdo before my semester exm without knowing the concept. Now I know about cDNA construction . Thankyou
Thymine nucleotide
Sir plz upload retroviral method ....i have to prepare that for my exams ..because of lockdown i was unable to attend any tuition or class from anyone .... Probably my offline exams gets started from November 20.. i have to prepare other subjects toooo ..plz sir help me 🙏
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S 1 nuclease cleave
Oligo dt primer
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How can I get these notes
Thank you bro for Crystal clear explanation could you pls provide the whatsapp link to join the group .I want the notes for this topic
I can't join the what's app group bcz it's full.....
Exons r coding sequence
Dna polymerase
Bhaii restriction mapping of DNA fragments and map construction topic par banaiye
Yeah already uploaded
those are not "cloned plasmids", it called as recombinant DNA
They are also called as cloned plasmids
Sir, can you plz resend the notes of this topic in the group... I just joined it
I am msc botany 4th student,, related video send me... 🙏
Awesome teaching.....
Thanks
Are the cDNA fragments single stranded or double stranded?
Double stranded
Reverse transcriptase
Brother me what's app group is full add kavdaniki avdamle
Hi sir, I just wanted to join in your WhatsApp grp for notes purpose but it showing unable to join It is full plz do something
Zabr 10 yar...
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Thanks
Alkaline naoh
5:04
Good concept... kindly can I get the link of wp group.
Where do I find whatsapp link for the notes?
Spilicing
Sir whatsaap no. ?
Talking slowly 🙏
Sir Plz share what's app link for notes
Bro whats app group link bro
Bhaii pdf milega iska
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