Fixing blurry images in Fiji/ImageJ (Deconvolution Lab2 plugin)
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- เผยแพร่เมื่อ 28 พ.ค. 2024
- A quick and easy way to try out different deconvolution routines in imageJ or Fiji. A quick guide to getting started. Time markers below;
0 -1min Intro
1.01 - Intro Video
1.17 - Data Prep
2.54 - Running the plugin
3.37 - Point Spread Functions
5.00 - Regularized Inverse Filter
5.52 - Iterative Constrained Tikhonov Miller
10.20 - Richardson Lucy
13.00 - Round up
Sir can you please expalin the step controllable (step y) parameter available in some of the algortihms
thank you for the video
i was wondering is one can user any psf ? in other words can i use a psf i find online for my images ? or do i have to create it by myself in my microscope ? if so , do you have any instructions on how to do it
Hi. No, it wouldnt be wise to use a psf from the internet. You dont need to if you know some basic parameters about your microscope or collection device. There is a plugin to calculate psf (Diffraction PSF) that works in both Fiji and ImageJ. C
@@CraigDaly yes thats correct. thank you for your reply. I did some research and found how to generate psf.
Thanks for the videos they are very helpful
hello sir, do u have an idea to quantify the green fluorescent Tunnel staining of mice tissue by Image J software. or by any other software. kindly help
Hi, could you have a look at this video to see if it answers your question. C. ‘Measuring intensity in a digital image (ImageJ)’
th-cam.com/video/_Ku2yXd4hcw/w-d-xo.html
Hello sir,
I am working on phases of PSAN/PMMA blends using Atomic force microscopy. Please upload a video about how to clean the phase images by image j so that we can easily differentiate PSAN and PMMA phases.
Can I do this for my images which I have taken using oil drop for bacteria? My images are slightly blurred no matter what I do I am not getting pinpoint images. Can I use deconvolution for my dataset?
Hi, if the images are blurry because they are out of focus then deconvolution may not help. The process models the distribution of light from a point source. If fluorescence convolution is not the reason for your blurriness then deconvolution is unlikely to help. C.
Hello sir,
Please tell me how you insert the scrolling icon to scroll the image right and left in the beginning.
Hi, are you working with a stack (z-series) of images? If so, the scroll bar appears automatically. So the scroll you see at the beginning of the video is scrolling through the depth (z-axis) of the data rather than to the right.