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thank you so much, its really helpful!!

Thompson John Gonzalez Christopher Thompson Elizabeth

Thank you so much! I have some questions, first, is it possible to train the classifier using more than one image? And second question, is using Stardist for training the model will give better results?

hii. i want to learn how to measure area of tympanic membrane perforations using image j software. please me guide me an approach how can i learn

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Walker Kenneth Anderson Jennifer Allen Dorothy

Lee Melissa Martin Jessica Lewis Jennifer

Gonzalez Betty White Karen Hall William

Allen Elizabeth Davis Robert Martinez Jose

Harris Jose Gonzalez Charles Jones Richard

Martinez Angela Lee Amy Rodriguez Brenda

Harris Elizabeth Williams Larry Jones Brian

Thanks for the perfect summary

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Brown Jessica Young George Miller Helen

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Jones Linda Perez Patricia Gonzalez Edward

Lewis George Johnson Brian Robinson Maria

Robinson Amy White Eric Garcia Laura

Lopez Helen Rodriguez Eric Thompson Angela

Walker Donna Wilson Brenda Thompson Carol

hello. I have a question.would you please help me?I want to count the number of grafts inside the plates with the Fiji (filament detector). how can i do that(can i have your email address i want to send photos of analysis)

hello. I have a question.would you please help me?I want to count the number of grafts inside the plates with the Fiji (filament detector). how can i do that(can i have your email address i want to send photos of analysis)

I have imaged gfp and rfp tagged protein and after merging both the channels, it is giving orange color, instead of expected yellow puncta. What could be the reason?

Hiii, sir craig! whenever I use Fiji to analyze the particles in my image, I set it to exclude images that are on the edges, but it still considers them. Has the same thing happened to you? Could you help me?

Great video ! All of the information are very useful :D

Quite insightful lecture,. Thanks

Very interesting!

Dear Craig, thank you for this informative video. I would like to ask when I try to set a threshold, I see the background as red, not the sample I stained. What could be the reason of this?

Terrific. Now I would like to learn how to quantify size.

Thanks

Hey Craig, I have been using chatgpt to enhance marco functionality instead of writing it from scratch and that is where I think it shines. For example I would record my work flow in Fiji with the macro recorder. Then I would give the code to ChatGPT and tell it I want to do this analysis in all files of a single folder. That works like magic

Great Ex-planation, helped a lot in my thesis!

Very helpful! Thank you :)

A very incredible concept!!

Great video, appreciated the explanations made this a lot easier to understand. Also love the Scottish accent!

Thanks for your video, I have a question How if the color of the cells and the background quite similar? Do you have another method for this case?

Excellent video! Thank you for making and sharing with us.

Your is THE BEST CHANNEL on TH-cam!!! Thanks a lot for all the videos you are making!!! 🙏

Hi, I followed this to make the macro. But somehow, my macro is usingthe same image again and again. Could you please help? Thanks

Where are you at with this project? I have some fun ideas to give you if you are still working in this field. I write grants if that helps too and know of a way to put this project and the main idea I wanna share into a format for field forensics and ER mop ups etc. If interested in rapping boot it reply and we’ll go from there.

Hi Criag, Thank you for the detailed videos, they are very helpful. I am a novice imageJ user and am trying to characterize spheroids (3D cellular circular structures) that are touching. The watershed function did not work the same way it did in this video. What would you recommend as a next step to try. Many Thanks, Brooke

I was missing the "set scale" part before setting scale bar, found it then on another tutorial

Hello! Thank you very much for this very clear tutorial! I used to do the thresholding manually in ImageJ, but now I want to automate it by linking Ilastik and ImageJ. Do you think it's possible to do it within a macro? if you could help me please ?

Hi, i am New in imagej and Hacer a question if could you answer please? İ am trying on one image that selecting multiple rois and want to for each roi apply crop, duplicate , add gray,Binary.......and skeletonise. Making it step bu step so bored. İ tried to make a macro but i failured. Can you help me please how can i handle. Thanks

This. Is. Awesome.

Hi Craig, thanks for the wonderful videos. Could you make a video on how to use ImageJ to analyse gray cast iron microstructure? For instance, the estimation of graphite flake length, volume fraction and size. If you need some images for the analysis, kindly let me know.

single-handedly saving me in the late stages in my master's once again. thank you sincerely so much for this content. <3