Gradient PCR: Theory and demonstration
ฝัง
- เผยแพร่เมื่อ 25 ส.ค. 2024
- In this video, we have tried to explain the theory and practical approach of gradient PCR. Gradient PCR is very useful to find out the correct annealing temperature by performing the Single PCR reaction. the optimal annealing temperature can be identified.
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Wow wat a mind blowing bro.. well explained, Keep doing... upcoming Biotechnology teacher... God bless you 🥰🥰🥰👍👍
Thank you so much sir, Good explanation 🙂
Well explained 👏
Wow...bhai tu grt teacher ban chuka hai
Informative 🙌
thank you straight forward
Thank you 😊
Well explained 👍 keep going 👍👍
Well explained and informative ❤️
Keep going 👏
Thank you sir❤
👌
Thank you for this, you the best :>
Thank you 🙂
Good ❤️
It is very clear explanation thankyou. Could you make a video for explain and read the results of melting curve after we used gradient method?
Yes, soon
👍👍
👌👏👏
Thanks for sharing this. right now I know that each row represent what temperature, i wonder that which column that I should put the PCR tube in? the first column of the row? or can put in any column of the row?
If ur doing gradient check for row and column with desired temperature , if ur doing normal PCR then keep position doesn’t matter
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Nice video. Well explained. If I have to do gradient pcr then I need multiple replicates for my sample right? So do we also need to keep positive and negetive control with them?
Really appreciated your 🙋♂️ question for a different temperatures u have to keep a negative control and positive control separately!
@@lifereplicon Thankyou for the reply. Can you also make or suggest any video or article, paper on how to clone cDNA into a vector?
Definitely 👍
Yes 🙌
❤