How to Prepare Stomata Slide | Microscopic Study of Stomata | Stomata Slide Preparation | ENGLISH

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This video is also available in Hindi. How to prepare stomata slide for microscopic study of stomata- हिंदी में • How to Prepare Stomata...
On the outer layer of the leaf of a plant are microscopic holes called stomata. Stomata control gas exchange and water loss by opening and closing. Stomata are of particular interest to plant breeders because plants with smaller or fewer stomata tend to have lower levels of evaporation and can survive drought better than those plants with more stomata. Additionally, researchers often study stomata for the effects of carbon dioxide and changes in atmospheric composition. In short, stomata are studied to measure any type of plant response to stress.
Normally stomata open in the morning and close during the night. However, not all plants open their stomata during the day. Some plants such as cacti and succulent plants open their stomata at night and close them during the day, in order to prevent losing too much water.
Stomata are usually found on both the top and the bottom of a leaf. Many plants have more stomata on the underside of the leaf. However there are exceptions, monocots, like grasses, have similar numbers on both the top and the bottom. Plants whose leaves rest on the surface of the water, like water lilies, often have very few stomata on the wet underside of their leaves.
There are basically 02 common methods for stomata view-
1- By peeling the upper layer of a leaf as we have shown in the video
2- If it is not possible then place a clear nail polish in it and after drying place a transparent tape over it and peel it and transfer it under a microscope to see the stomata.
Apparatus and Materials Required:
Sampl leaves, forceps, needles, watch glasses, glass slides, a dropper, coverslips, a brush, blotting paper, safranin, glycerine and a compound microscope.
Procedure:
1. Remove a healthy leaf from the potted plant.
2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf over and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing water.
3. Put a few drops of safranin stain in a watch glass.
4. After 2-3 minutes take out the peel and place it on a clean glass slide.
5. Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle.
6. Remove the excess stain and glycerin with the help of blotting paper.
7. Observe the slide under the low-power and high-power magnifications of the compound microscope.
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[@aparnapeshkar1748]

For vinca rosea leaf, how should we tear it so that we get that transparent layer

[@puchalapallisaisireesha8128]

Thank you sooo much for your clear explanation madam

[@askaaaaaaaaaaaa1318]

I am happy for that your English explanation video separately. 🙏🙏🙏

[@solutionpharmacy]

Thank you

[@prashantbhosale-patil6614]

Very informative video with practical ....Actually good...

[@boikuntho2489]

Which plant leaf will be good for this practical?
Name some easily available plant leaves

[@solutionpharmacy]

Datura vinca vasaka

[@scenturion]

From the video, the lower layer of the leaf was peeled. Your note says otherwise ( number 1 method).

[@timothydestiny3865]

Thank you so much ma'am

[@sanmathihegde6903]

Thank you so much !!
Really helpful

[@Ahktermir]

Nicely explained. 👏👏👏👏

[@solutionpharmacy]

Thank you

[@meghagupta6462]

Mam plz tell can we use 100x ,200x,400x magnification

[@timothydestiny3865]

Yes. It will make it even bigger and clearer

[@ariviyalkalanjiyam]

Very nice presentation mam 👍

[@solutionpharmacy]

Thank you so much for your feedback and support. Please download our app from play store - play.google.com/store/apps/details?id=co.bolton.nqilv

[@sehajdeepkaur5911]

Mam..which leaf is used here??

[@gracegorgeous3813]

Thank you

[@ekemini_andrew]

What type of stomata is this

[@Meghana..1507]

Thank you mam

[@solutionpharmacy]

Keep watching

[@umeshchandragahtori9519]

Mention the leaves please

[@solutionpharmacy]

Vasaka

[@RenkaHitnalli-p7c]

Name of leaf

[@ManjeetKaur-og3yn]

Thanku ma

[@sathyasrinivas8569]

Thank you so much mam . Nice explaination

[@devlinasengupta6780]

Very good

[@SJ-yu9ux]

Thank you so much ma'am ☺️ great explaination 👍

[@sadiahussain3000]

Which red chemical is she using?

[@solutionpharmacy]

Safranin solution

[@sssudips]

Thank you soooooo much

[@garrysingh385]

Which is in 2 Beaker.

[@shreyapandey2005]

Safranin is in watchglass 2

[@nakalchibandar4818]

Nice

[@shakunthalab2492]

Thanks

[@sonal0036]

Kiski leaf h yeh????

[@solutionpharmacy]

Vasaka

[@randeepkaurcreativity8373]

Nice teaching

[@solutionpharmacy]

Thank you

[@khangelathandsipho6052]

can someone please tell me why we use Dillute Sulfuric Solution

[@Querysdyukihafthsnwou]

Timepass

[@ethanwestwood3168]

To stain the stoma

[@shrivanth5896]

@@ethanwestwood3168 you can even use safranin

[@gsbiradar6265]

Tmr is my practical exam

[@gsbiradar6265]

Thnx

[@conceptbiology360]

Thank u mam

[@darkgamerdg1762]

👍

[@FireShadeZ]

Ok

[@Tejasiwini___]

👍🤠

[@naraparajulakshmianuradha5202]

2nd comment........ Nice video

[@naraparajulakshmianuradha5202]

Nice huh...

[@solutionpharmacy]

Thank you

[@YaaVaalaa]

Hindi ma bolo

[@solutionpharmacy]

Hindi me bhi hai isi ka video search kariye

[@YaaVaalaa]

@@solutionpharmacy theek hai

[@Mahalakshmi-tb4yh]

thank you mam

[@chiku9337]

Thnx

[@koyanasamanta6347]

Thank you mam