Transmission electron microscopy principle and working (TEM)

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  • เผยแพร่เมื่อ 30 ก.ย. 2024

ความคิดเห็น • 177

  • @mohammad_yazbek
    @mohammad_yazbek 4 ปีที่แล้ว +130

    A biology master! Fantastic job Shomu! I attend the University of Michigan and I watch your videos. Your presentations are scientifically accurate and precise. Hello from the United States of America.

  • @nobodywatchesnooby
    @nobodywatchesnooby 4 ปีที่แล้ว +185

    It's true that everything is easier to learn if the indian guy on youtube explains it

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว +10

      Thank you

    • @lovepunjab5429
      @lovepunjab5429 3 ปีที่แล้ว +3

      But no guy can compete shomus . Love From Pakistan😍

    • @humairaniamat9349
      @humairaniamat9349 3 ปีที่แล้ว +5

      @@lovepunjab5429 yes he is the best in teaching. But I recommend to watch Dr. Najeeb the best best best teacher in the world I must say.

  • @LordOfFries3
    @LordOfFries3 4 ปีที่แล้ว +31

    I’m studying nanotechnology and your videos have helped me so much! You explain complex topics in a way I completely understand! Thank you!!

  • @susmitap1203
    @susmitap1203 2 ปีที่แล้ว +15

    Excellent job!! I am from Canada and I find your classes precise, lucid and minus jargon!! keep making great educational videos.

  • @sruthivibin326
    @sruthivibin326 3 ปีที่แล้ว +3

    Tnq sir...✨️ hii from kerala

  • @nazrulislam-ol8wt
    @nazrulislam-ol8wt 4 ปีที่แล้ว +5

    Amazing explanation my brother . You have this wonderful way of explaining complicated things in ease .

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @JawadAli-hs2qm
    @JawadAli-hs2qm 3 หลายเดือนก่อน +2

    You indians are very talented.....sir g love from Pakistan ❤

  • @AngelElizabethAbraham
    @AngelElizabethAbraham 26 วันที่ผ่านมา +1

    Incredible Loved it ....This helped ne to figure out my A level topic (UK)

  • @IcarusLife
    @IcarusLife 3 ปีที่แล้ว +7

    Wow... Totally understandable❤

  • @gamshadroshan6032
    @gamshadroshan6032 4 ปีที่แล้ว +5

    Hello sir, thank you for your amazing lecture... I just have a Q.. in case of TEM , why eye EML is before detector? How we can see the image befor detector ? .....

    • @sudhamaydinda724
      @sudhamaydinda724 2 ปีที่แล้ว

      That is not eye,,,He is talking about eye piece lens actually,,

    • @bvbreddy68
      @bvbreddy68 2 ปีที่แล้ว

      you can not see actual specimen with your eyes, what you see is shadow or image of specimen in black and white .This is due to negative staining of grids with urynyl acetate. electrons pass through copper grid on which sample or thin sections are placed.there is no eye lenses in TEM.

  • @girijaprabhakaran8286
    @girijaprabhakaran8286 4 ปีที่แล้ว +4

    Thank you so much sir . Most of the questions for my examination are mainly from these portions so it really helps thank you again for this wonderful explanation.

  • @akhtaruzzamanbandhan6552
    @akhtaruzzamanbandhan6552 2 ปีที่แล้ว +7

    Believe me or not.
    I am doing my MS in POSTECH, South Korea.
    Your video helps me a lot fo my research.
    Love and respect from South Korea. You are amazing

  • @jincyacademy1999
    @jincyacademy1999 2 ปีที่แล้ว +2

    Thank you sir 🙏

  • @dr.prabhajeeva9867
    @dr.prabhajeeva9867 หลายเดือนก่อน +1

    Precise teaching

  • @sisirbarik1237
    @sisirbarik1237 4 ปีที่แล้ว +1

    Sir ami kamon kre bhu msc Zoology entrance exam 2019 question paper r answer key pabo?please bolben.

  • @veronicapasquariello3173
    @veronicapasquariello3173 2 ปีที่แล้ว +3

    Your explanation is simple and very clear, thank you so much!

  • @kathikaghosh5789
    @kathikaghosh5789 6 หลายเดือนก่อน +1

    Thank you sir it's very helpful ❤

  • @full2fullroasting980
    @full2fullroasting980 4 ปีที่แล้ว +3

    Sir ur Videos are very useful...i am biotechnology student and in this semester we have microscopy...during this quarantine ur Videos are so much useful to us..Thank you

  • @roshnibhatt2801
    @roshnibhatt2801 3 ปีที่แล้ว +2

    In pendemic situation I saw all your videos on the basis of biology topics, all are more important for different types of exams, now I 'm easily understood the light and electron microscopy and TEM OR SEM, Thankyou so much sir.

  • @dr.prabhajeeva9867
    @dr.prabhajeeva9867 หลายเดือนก่อน +1

    Im microbiology professor simply super

  • @mltbyindrani
    @mltbyindrani 3 ปีที่แล้ว +2

    easy and smarty taught thanks a lot 🙏🙏🙏

  • @varshasadhwani1452
    @varshasadhwani1452 4 ปีที่แล้ว +1

    Sirji mujhe sample preparation of scanning electron microscope and transmission electron microscope par chahiye vedio please bna dijiye

  • @lime3620
    @lime3620 ปีที่แล้ว +1

    Hi, great video! I just want to point out that generally 200 kV is used rather than 20 kV.

  • @ozgulaliya7613
    @ozgulaliya7613 4 ปีที่แล้ว +1

    I think u sould tell about how we obtain the black and white images...😖

  • @bts_rollercosterlifepurplearmy
    @bts_rollercosterlifepurplearmy ปีที่แล้ว +2

    After every lacture of science, i glad that i choose Science field.....
    Bcz every simple things has lots of reasons and has their own working principles which makes us surprise about how's the things are actually going...
    And the way U just teach us in simple and accurate manner is like सोने पे सुहागा 🌟
    So thanks for sharing knowledge ...

  • @arshadgour6457
    @arshadgour6457 2 ปีที่แล้ว +1

    Thanks meri samajh me achha aaya me hindi midium se hu

  • @swapnalikamble5753
    @swapnalikamble5753 3 ปีที่แล้ว +1

    Nice explaination 😍😍aur aap bhi bohot ache dikh rhe ho😂😂🙈😍 itne ache sir humko kbhi mile hi nai school clg mai 😂😂😀milte to hum bhi top krte lec baithke😂😂😂😂

  • @varshasadhwani1452
    @varshasadhwani1452 4 ปีที่แล้ว +1

    Sir please help me 🙏

  • @dharavathnikitha4151
    @dharavathnikitha4151 9 หลายเดือนก่อน +1

    Nice explanation sir

  • @demon_12355
    @demon_12355 8 หลายเดือนก่อน +1

    Very helpful sir🥰

  • @dr.mukhtarahmed8579
    @dr.mukhtarahmed8579 ปีที่แล้ว

    Presentation is superb. At the same time cutting sample with glass knife and diamond knife is not easy as you have explained in your presentation. Even sample preparation is a tedious job. If fixation and preparation is not good your whole procedure will collapse and it will impact your results. From A to Z it should be accurate and dust free environment.

  • @rawanmhajne6822
    @rawanmhajne6822 10 หลายเดือนก่อน +1

    Thank you sir!

  • @mutaherashaikh2541
    @mutaherashaikh2541 4 หลายเดือนก่อน +1

    And i found that you have made whole biology topics lecture..which is helping us a lot.

  • @mutaherashaikh2541
    @mutaherashaikh2541 4 หลายเดือนก่อน +1

    I watch so many videos from others but lastly come to your lecture then only i can clear my concepts..

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 หลายเดือนก่อน

      Next time, come first and you don't need to visit any other place

  • @MeenuBagariya
    @MeenuBagariya 3 ปีที่แล้ว +2

    👌👌👌👌👌👌👌✍️✍️✍️✍️✍️✍️🙏🙏🙏🙏🙏

  • @mansipunnase
    @mansipunnase ปีที่แล้ว +1

    Thank you sir, your explaination is simple and easily understandable, this videos is helpful for us🙏🏻

  • @bvbreddy68
    @bvbreddy68 ปีที่แล้ว

    he missed negative staining samples with urynyl acetate with acidic pH , 300 mesh copper grids, coating of grids with carbon is essential for retaining sample and passing of electrons. many more practical things are involved in TEM. any way good lecture

  • @adelyayakupova6616
    @adelyayakupova6616 ปีที่แล้ว +1

    THANK YOU SO MUCH

  • @ameeshakushwaha9302
    @ameeshakushwaha9302 2 ปีที่แล้ว +1

    Thank you so much

  • @tuhinakhtarakash2624
    @tuhinakhtarakash2624 ปีที่แล้ว +1

    Sir, Because of you I am able to score good in the exams. X-Rd which u have taught I have written in exam and got 14 out of 16

  • @AwaisAkram7777
    @AwaisAkram7777 4 ปีที่แล้ว +1

    Hi brother, How we can prepare the sample of both SEM and TEM ???????????

    • @AwaisAkram7777
      @AwaisAkram7777 4 ปีที่แล้ว

      And can you make the Separate video about sample preparation????

  • @UdhayaKumar-nj1wi
    @UdhayaKumar-nj1wi 10 หลายเดือนก่อน +1

  • @SABIANS85
    @SABIANS85 4 ปีที่แล้ว +1

    Ur vedios are sooo soo much good ....i alwyz listen ur vedio whn there is any prblm in my studies .....caryy on ...good work👍.....i really appreciate ur efforts 🤗😍

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      Glad to hear that you're getting benefit from my lectures. Please subscribe and share

  • @motapothulasneha9605
    @motapothulasneha9605 10 หลายเดือนก่อน +1

    Thanks!❤

  • @smritisharan8961
    @smritisharan8961 4 ปีที่แล้ว +1

    Very much informative. Thanks sir. If possible plz make a video on chromosome painting.

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @heartandsoul4202
    @heartandsoul4202 11 หลายเดือนก่อน +1

    A big like to your vids from Ethiopia.

  • @anjuganapriya7735
    @anjuganapriya7735 4 ปีที่แล้ว +1

    need specimen preparation....

  • @shashikantverma60
    @shashikantverma60 2 ปีที่แล้ว +1

    nice

  • @jassgill556
    @jassgill556 11 หลายเดือนก่อน +1

    Sir you expalined the things in very easy way beeter than the lecturers in college ty ty for your efforts

  • @sradhanjalibehera7223
    @sradhanjalibehera7223 2 ปีที่แล้ว

    There should be a fluorescent screen to see the polychromatic light . But you haven't explained that..

  • @Mrsqueen-ql2pi
    @Mrsqueen-ql2pi 9 หลายเดือนก่อน +1

    Thanku sir ❤

  • @DenLargo-zp4cp
    @DenLargo-zp4cp 9 หลายเดือนก่อน +1

    Привет из Сколтеха

  • @divya-c6v
    @divya-c6v ปีที่แล้ว +1

    thanku sir

  • @rrameshnithesh2901
    @rrameshnithesh2901 2 ปีที่แล้ว

    Sir How the images of Small cells are taken from the Microscope.. us there any specialized camera??

  • @tuhinakhtarakash2624
    @tuhinakhtarakash2624 ปีที่แล้ว +2

    Thank Sir

  • @rawanmhajne8568
    @rawanmhajne8568 2 ปีที่แล้ว +1

    Mr. Shomu, thank you very much for this lecture. Very accurate and simple at the same time. It will be fantastic to give us examples and images for different particles\material. One last thing that I would like to hear from you is the staining of the samples if the atoms are not "heavy" enough to give us a signal. I appreciate your work!!

  • @aparnareghukumar2000
    @aparnareghukumar2000 3 ปีที่แล้ว

    Transmission electron microscopy and tunneling electron microscopy are same????

  • @aryasahay
    @aryasahay 7 หลายเดือนก่อน +1

    Extremely good explanation💯

  • @shrutiaich8908
    @shrutiaich8908 3 ปีที่แล้ว +1

    Very nice ..

  • @rinivishwakarma7497
    @rinivishwakarma7497 2 ปีที่แล้ว

    why heavy element atom aborsb more electron amd vice versa?
    pls explain me, if possible

  • @edwinkopelli2750
    @edwinkopelli2750 ปีที่แล้ว +1

    Thank you sir, helped me to learn in short time😊

  • @chami778
    @chami778 2 ปีที่แล้ว

    ,sir, specimen splicing by using dimond knof in this microscopy?

  • @bhavyathatituri6192
    @bhavyathatituri6192 4 ปีที่แล้ว +1

    Thank you sir, am fallowing all the series of this lecture, and am clear abt the topics🙏🙏

  • @ebinjoe5798
    @ebinjoe5798 2 ปีที่แล้ว

    Sir I have a doubt so in TEM if there is no +ve anode to control the diffraction of electron beam then how does it diffract properly?

  • @053metveeramallavamshi2
    @053metveeramallavamshi2 2 ปีที่แล้ว

    For non metallic materials which polishing machine is used for TEM

  • @hodongjunior2826
    @hodongjunior2826 2 ปีที่แล้ว +1

    Thank you so much! your explanations are easy to understand!

  • @eunicenaakoshie7954
    @eunicenaakoshie7954 ปีที่แล้ว

    please does the heavy atoms in the sample absorb or deflect the electrons?

  • @23jyotiratnani92
    @23jyotiratnani92 2 ปีที่แล้ว

    Sir Can you please share previous lecture link?????

  • @sardarrafaqatrafaqat7127
    @sardarrafaqatrafaqat7127 ปีที่แล้ว

    Than

  • @iamstay1257
    @iamstay1257 ปีที่แล้ว +1

    Aapke vajhese pass ho jayenge sir! Thank you 🙏

  • @Shells12-v5c
    @Shells12-v5c 2 ปีที่แล้ว +1

    Thank you so much.. 🤞

  • @somamughal7120
    @somamughal7120 3 ปีที่แล้ว

    what is difference between Transmission and reflection electron microscopy??

  • @subhasmrutipanda7916
    @subhasmrutipanda7916 ปีที่แล้ว

    Sir please make a video on Scanning tunneling microscopy and atomic force microscopy 🙏🏻🙏🏻

  • @ncertsciencebylaxman8502
    @ncertsciencebylaxman8502 4 ปีที่แล้ว

    सर् हिंदी मैं भी करवाइये।

  • @23jyotiratnani92
    @23jyotiratnani92 2 ปีที่แล้ว

    Last lecture link!???

  • @shifnahameed4008
    @shifnahameed4008 2 ปีที่แล้ว +1

    God bless you❣

  • @Falakshorts751
    @Falakshorts751 2 ปีที่แล้ว

    Video thumbnail is different , it is SEM but in video explanation is about TEM, kindly rectify it . best video.👍🏻👍🏻

    • @shomusbiologyofficial
      @shomusbiologyofficial  2 ปีที่แล้ว

      Thumbnail says it's TEM

    • @Falakshorts751
      @Falakshorts751 2 ปีที่แล้ว

      @@shomusbiologyofficial you are right . I m sorry , but there was some optical illusion on my device , or may be I got it mistakenly. thank you

  • @MrRisha100
    @MrRisha100 3 ปีที่แล้ว

    Good Lecture... can we analyze live cells in cell culture with TEM? Is it doable?

  • @slokrathor285
    @slokrathor285 2 ปีที่แล้ว +1

    What a easy explanation Mann❣️🔥

  • @anandnandu245
    @anandnandu245 2 ปีที่แล้ว +1

    Thank u 🔥

  • @manishayadav-oc9rp
    @manishayadav-oc9rp 4 ปีที่แล้ว

    Sir please make video on why dna required rna primer. Humble request🙏😊 to u

  • @geethulakshmi757
    @geethulakshmi757 2 ปีที่แล้ว

    Sir, can you please upload specimen prepration in TEM..🙏🙏

  • @kvnkvn9191
    @kvnkvn9191 3 ปีที่แล้ว

    Sir I'm confused here 7:17 electrons are absorbed or scattered by repulsion? 🤔

  • @anonymousplayer6378
    @anonymousplayer6378 2 ปีที่แล้ว

    Sir, can you make a video on cryoEM if possible?

  • @harshgami9351
    @harshgami9351 4 ปีที่แล้ว

    Hi sir
    Sir I want tell u that please help us to remember examples of chp wise for neet perpetration
    Plz help us.
    It difficult to remember all examples for neet exam
    And give some shortcut to remember examples 🙏

  • @jabifatma2844
    @jabifatma2844 3 ปีที่แล้ว

    Sir, if we tag the specimen with flourscent ....then is it possible to get colour image through TEM??

  • @sarathambalc5458
    @sarathambalc5458 4 ปีที่แล้ว +1

    Nice

  • @rutujameheshre8392
    @rutujameheshre8392 3 ปีที่แล้ว

    Why compare the light microscope to electron microscope

  • @malikareeb45
    @malikareeb45 4 ปีที่แล้ว +1

    Thank you so much sir ☺️

    • @shomusbiologyofficial
      @shomusbiologyofficial  4 ปีที่แล้ว

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @manyloonies9157
    @manyloonies9157 4 ปีที่แล้ว

    Interesting lectures. Please can you explain if using PCR to find sequence of interest, say a viral sequence, the primer and probe alone will somehow stick to complementary sequence in a crude sample with many mixed sequences of different lengths and nucleobases. Am confused by the terminology used calling the target sequence of interest a template seems to imply a predesigned sequence is also added into the PCR process. Grateful if you can clarify. Thanks.

  • @MrSa-gk4oe
    @MrSa-gk4oe 4 ปีที่แล้ว

    Tem is the test kit ??for corona

  • @yashikanalla7511
    @yashikanalla7511 4 ปีที่แล้ว +1

    👌🏽well explained

  • @harinisrinivasan5891
    @harinisrinivasan5891 4 ปีที่แล้ว

    Sir pls post phenol coefficient test

  • @mid7699
    @mid7699 4 ปีที่แล้ว

    Please make a video on research opportunities after class 12.

    • @mid7699
      @mid7699 4 ปีที่แล้ว

      Exams list.

  • @imankitroy5865
    @imankitroy5865 4 ปีที่แล้ว

    Why this video is not available in different speed

  • @janujaj271
    @janujaj271 4 ปีที่แล้ว

    Sir difference between SEM & TEM?

  • @raghad8348
    @raghad8348 3 ปีที่แล้ว +1

    Thanks!!
    I have a question if you please, how long does it take to shoot the sample?

  • @manoj87874
    @manoj87874 4 ปีที่แล้ว

    Sir bilingual padhaya kro please

  • @yo_raaz7180
    @yo_raaz7180 4 ปีที่แล้ว

    Thank you sir thank you so much ❤️

  • @jassgill556
    @jassgill556 11 หลายเดือนก่อน

    Sir plzz give us some ppts for bsc and msc level students for subjective papers