Gel filtration chromatography in 5 minutes | Size Exclusion Chromatography | Lab Procedure

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  • เผยแพร่เมื่อ 27 พ.ย. 2024

ความคิดเห็น • 64

  • @NEBULAFITNESS9885
    @NEBULAFITNESS9885 ปีที่แล้ว +7

    This video was was helpful for me in microbiology , thanks sir 🙂

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  ปีที่แล้ว

      Please subscribe to my second channel where I upload career advise youtube.com/@letscrackbioexams94?si=Hhovo7iVObYqyZ5l

  • @nikolaspais9029
    @nikolaspais9029 4 ปีที่แล้ว +24

    thank you so much i study biochemistry and this video was really helpful. keep up the good work!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 ปีที่แล้ว

      Please share my channel link with your friends and help me to reach big audience

    • @maham671
      @maham671 ปีที่แล้ว

      Me also studying biochemistry

  • @PriyankaPrasad0811
    @PriyankaPrasad0811 9 หลายเดือนก่อน +1

    Bro thank you for this, like genuinely. I'm currently cramming for my first biochem exam and you did in five minutes what my professor couldn't do in two weeks :P

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  9 หลายเดือนก่อน

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @generendellaquino5944
    @generendellaquino5944 3 ปีที่แล้ว +7

    Thank you so much for this! Keep up the good work :)

  • @shahabkhan7604
    @shahabkhan7604 10 หลายเดือนก่อน +1

    Would be nice if you add lil bit description, written form as summary, one liner so we may revise things from that

  • @abhiruchisah1595
    @abhiruchisah1595 2 ปีที่แล้ว +1

    thank you .You explaine it very nicely .Im from iiser tirupati pursuing majors in biology

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 ปีที่แล้ว

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
      I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
      You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

  • @KCGLOBAL2024
    @KCGLOBAL2024 3 หลายเดือนก่อน

    I love this
    Please don't stop now that I'm in school

  • @SulekhaJharimune
    @SulekhaJharimune 3 หลายเดือนก่อน +1

    Amazing work!! Keep it up 👍

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 หลายเดือนก่อน +1

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @avneetkalra1145
    @avneetkalra1145 3 ปีที่แล้ว +2

    thank u for making it alot easier

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 ปีที่แล้ว

      Please share my channel link with your friends and help me to reach big audience

  • @laibanadeem2664
    @laibanadeem2664 6 หลายเดือนก่อน +1

    You explained really well....

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  6 หลายเดือนก่อน

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

    • @laibanadeem2664
      @laibanadeem2664 6 หลายเดือนก่อน

      @@animatedbiologywitharpan ok

  • @KokoriKid1
    @KokoriKid1 ปีที่แล้ว +1

    Great video man

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  ปีที่แล้ว

      Please share my channel link with your friends and help me to reach big audiance

  • @udayselva1476
    @udayselva1476 ปีที่แล้ว +2

    Good job brother 💓👌👍

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  ปีที่แล้ว

      Please support my channel via super thanks. Your small contribution like 1$ means a lot for us. The super thanks option is present at the lower right corner of any video, a heart sign with $ in it.

  • @kimpereira4770
    @kimpereira4770 3 ปีที่แล้ว +2

    This helped so much! Thank you!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 ปีที่แล้ว

      Please share my channel link with your friends and help me to reach big audience

  • @prakashvincent9836
    @prakashvincent9836 4 ปีที่แล้ว +5

    Hi your explanation is good. Just a concern is, in the 0.33 time, you tell that there is binding of proteins in gel filtration column which is wrong

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 ปีที่แล้ว

      I think you got me wrong @ .33 sec I was talking about a general workflow of column chromatography and later pointed out that GFC is bit different than the general workflow ,it involves seiving mechanism in contrast to binding

  • @debarghyapratimgupta9487
    @debarghyapratimgupta9487 4 ปีที่แล้ว +1

    Great.. Thank you!

  • @hccc8782
    @hccc8782 8 หลายเดือนก่อน +1

    very useful

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  8 หลายเดือนก่อน +1

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @syedtoufeeqahmad9023
    @syedtoufeeqahmad9023 3 ปีที่แล้ว +2

    Thank u sir

  • @palvoliuto6059
    @palvoliuto6059 3 ปีที่แล้ว +2

    thank you for your great videos. could you please answer a question for me. what type of chromatography would you recommend for filtration of a polypeptide fragment? Thank you.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 ปีที่แล้ว +2

      If the peptide fragment is previously tagged by his tag / HA tag then one can do affinity chromatography, if slight estimation of size is known then one can do gel filtration, if amino acid composition is known and it has charged residues then one can go for ion exchange as well. So it really depends on what we know about the polypeptide.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 ปีที่แล้ว +1

      Please share my channel link with your friends and help me reach out to big audience

    • @palvoliuto6059
      @palvoliuto6059 3 ปีที่แล้ว

      @@animatedbiologywitharpan Thanks you so much my friend! All the best!!!

    • @palvoliuto6059
      @palvoliuto6059 3 ปีที่แล้ว

      @@animatedbiologywitharpan i will and i have subscribed too!!

  • @Suraj_gawali
    @Suraj_gawali 2 ปีที่แล้ว +1

    I watching your playlist

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 ปีที่แล้ว

      Please support my chabnel such that i can bring more cobtents like this. You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution ( starts from 40rs) means a lot for me.

    • @Suraj_gawali
      @Suraj_gawali 2 ปีที่แล้ว +1

      What is your education.and what perpose of making vidio

    • @Suraj_gawali
      @Suraj_gawali 2 ปีที่แล้ว +1

      @@animatedbiologywitharpan bro where are you form ? Give me your Mobile no.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 ปีที่แล้ว

      Go to the about section in the channel you would get everything.

  • @patriciajimenez492
    @patriciajimenez492 3 ปีที่แล้ว +2

    can the elution volume be greater than the total volume of the column?

  • @sammrayounas2681
    @sammrayounas2681 4 ปีที่แล้ว +1

    Suppose if exclusion range is 5 to 600 then the molecules whose size is below 5 they also trapped between the voids then these molecules also eluted with small size molecules then our reqired size protein will not be obtained is this happen or not sir?????
    Kindly tell me...

  • @laiqaaftab8555
    @laiqaaftab8555 ปีที่แล้ว +1

    what is wash in workflow

  • @khadojaraad
    @khadojaraad 2 ปีที่แล้ว +2

    Thank you ,can you put translating in to Arabic?

  • @ericajanebone9609
    @ericajanebone9609 หลายเดือนก่อน +1

    Whats elute

  • @vietthangduong7629
    @vietthangduong7629 3 ปีที่แล้ว +1

    god blesses me pass my text. make my teacher see this cmt plz T.T

  • @ethanferrer1577
    @ethanferrer1577 3 ปีที่แล้ว +2

    Redemption

    • @nerocean09
      @nerocean09 3 ปีที่แล้ว

      yoooo my slime, i know you dont know mandem like that

  • @awantikatiwari8080
    @awantikatiwari8080 4 ปีที่แล้ว +1

    What does that G represents in that beads chart

  • @PrinessSonali
    @PrinessSonali 4 ปีที่แล้ว

    Sir how to score well in sec C?

  • @prasadpitagi8787
    @prasadpitagi8787 3 ปีที่แล้ว +1

    The explanation and animation are great, but your accent in irritative. At some point of time in this short video it drew my attention away. Speak either in Indian accent or western.

    • @babangapu8189
      @babangapu8189 3 ปีที่แล้ว

      So is your accent british? Or like your idol sachin tendulkar

  • @harmohitkaur9867
    @harmohitkaur9867 4 ปีที่แล้ว +2

    yo can you not use the r slur thank you. if you don’t know why it’s offensive even in a scientific context, look it up.