It's so cool that I just discovered your channel, while I'm studying for my biochemical analytics exam, and all your new videos are about my exam topics :)
Excellent video! I have a question regarding the techniques prior to SEC. Would it also be compatible to develop an HPLC-TOF-MS method to characterize an antibody and then purify it using SEC?
Yes, at least to me that sounds like a good idea! The mass spectrometry will give you the relative mass values which I believe you can use to determine the "pore size" of the beads of the size exclusion chromatography. However, I might be wrong as I'm not super familiar with the more practical aspects of size exclusion chromatography.
Interesting question. I must admit I don't know for certain but I'm sure as with almost all things in life that it's not a 100% perfect system. So yes, I do believe that happens sometimes.
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If you have any questions, don't hesitate to ask me in the comments!!😇👍
You are the goat! Ur videos helped me a lot thank you!
Thank you so much for the nice comment!
It's so cool that I just discovered your channel, while I'm studying for my biochemical analytics exam, and all your new videos are about my exam topics :)
That's really great to hear! Let me know if there are other topics you want me to cover in the future😇👍
@@LucasLearnz sure, OUR, OTR, DO would be cool. In general fermentation, for extracellular and intracellular products.
I just had a course all about it! I found it quite challenging but I'll do my best to explain it in a video soon! 👍
Excellent video! I have a question regarding the techniques prior to SEC. Would it also be compatible to develop an HPLC-TOF-MS method to characterize an antibody and then purify it using SEC?
Yes, at least to me that sounds like a good idea!
The mass spectrometry will give you the relative mass values which I believe you can use to determine the "pore size" of the beads of the size exclusion chromatography.
However, I might be wrong as I'm not super familiar with the more practical aspects of size exclusion chromatography.
Will some of the small molecules still escape with the bigger one?
Interesting question. I must admit I don't know for certain but I'm sure as with almost all things in life that it's not a 100% perfect system.
So yes, I do believe that happens sometimes.
1:47 hahhahahah