Autoclave = Sterilization Process of Glassware & Nutrient Media by Moist Heat Sterilization (HINDI)

This video is also available in English Autoclave- General Construction and Working Principle- Moist Heat Sterilization (English). • Autoclave | General Co...
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Autoclave- General Construction and Working Principle- Moist Heat Sterilization (English)
The autoclave is a container similar to a pressure cooker. It is filled with the objects to be sterilised and then sealed. Next, high-temperature steam is forced in under high pressure, thereby displacing air. Moist heat destroys microorganisms by the irreversible coagulation and denaturation of enzymes and structural proteins. The time and temperature to accomplish this depending on pressure and the type of microorganisms to be inactivated. After the necessary time has passed, the steam is released and the sterilised objects are removed. The entire cycle can take from 15 to 60 minutes (batch processing).
A more commonly used method when extended heat is not a concern is to use an autoclave or pressure cooker. When sterilizing in this way, samples are placed into a steam chamber on a shelf or raised floor, and the chamber is closed and heated so that steam forces air out of the vents or exhausts. The pressure is then applied so that the interior temperature reaches 121 °C (250 °F), and this temperature is maintained for between 15 and 30 minutes. This elevated temperature and pressure are sufficient to sterilise samples of any commonly encountered microbes or spores.
This video will help you to understand- autoclave working, the principle of an autoclave, sterilization process, the importance of sterilization, sterilization process, moist heat sterilization, working of an autoclave, construction of autoclave, how autoclave works,
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[@Indrajit-DN94]

Visual perception is so much more effective
Thanks a lot

[@shraddhadubey4276]

Ur voice directly penetrates to the brain clearing the whole topic.!!! Thank you mam.

[@premwele9440]

Yes mam I also like your teaching explanation thanks mam

[@hazarikahemanta3760]

Bohot atxa laga vedio ko dekh kr or atxyei se samas me a gaya..tqq

[@shoyabmasuldar2856]

Very nice, understanding me sooo much tq😊👍👍

[@bilalahmad6102]

nice good job help full video ma'am thnks.. 👍

[@Automationstudy]

Very nicely explained

[@user-bo7bh5lt1m]

Thank you for uploading this video

[@solutionpharmacy]

Most welcome

[@poojakamble7292]

Thank you so much mam 🙏😊

[@ruchika_899]

Tysm ma'am 💓

[@_Aasiya_]

thank you so much mam 💖

[@gururajagvt1582]

Thank you somuch

[@dhanvindpk8276]

Very neatly explained..thank you

[@bipss3365]

I'm obliged🙏🏼🙏🏼

[@artistrajesha.r.k.v6442]

Thanks ma'am

[@KuldeepKumar-tn2bo]

Very nice

[@sudhanshuranjan1881]

Mam and sir you are the best.. you are doing invaluable job for students... thanks

[@solutionpharmacy]

SUDHANSHU RANJAN Thank you so much for your valuable feedback. Its motivate us to give our best

[@girdharilalpareek2098]

5d5 5

[@kulaman1564]

Thx

[@futuremotivation9266]

Thank you ma'am

[@aarzoofiza700]

Helpfull❤

[@mamta5026]

Nyc mam guiding

[@shaiklathifmunnisha1426]

Tq so much mam

[@jinalvanjara5240]

Thankyou tommorow is my exam n l missed this practical during classes
Big help😊❤️

[@mayankrai772]

Isse achha me bata sakta hu

[@crazyqueen.7212]

Thank you so much mam..
U are a saviour❤

[@solutionpharmacy]

All the best

[@rsingh1508]

Thanks broh

[@KuldeepKumar-tn2bo]

❤️❤️❤️❤️Good

[@rahulporadhiya3032]

Thanks madam

[@Satendra.maurya]

thanks

[@mohanrock5810]

Nice voice....

[@rahulporadhiya3032]

Mam thanks

[@simon1793]

Mam, mera whoicel kam nehi korta.but sefty valve sey pressure bahar hota hey 15 psi may. Same autoclave. Hoi problem hogi?

[@vaishnavigopaltupkar2306]

Very helpful 😍 tysm mam

[@rajnipuri912]

Where to get gasket for this autoclave

[@aashishraj3620]

Thankyou for tjis video book is boring..
Video is very interesting and informative thank you

[@solutionpharmacy]

Thank you

[@BITTUKUMAR-eb9oy]

Very nice mam

[@solutionpharmacy]

Thank you

[@PreetiSharma-bi4me]

What about vacuum releasing vent

[@aventadorgamer1575]

Helpful

[@solutionpharmacy]

Thank you

[@forknowledge9162]

Nice video

[@solutionpharmacy]

Thank you

[@rishabhchourasia5697]

which autoclave is this and where can I buy it? Can you please help me ma'am.

[@Rajeevkumar-dx2up]

Nice vedio

[@solutionpharmacy]

Thank you

[@surajchougule4351]

What is autoclave size ltrs ?

[@Dearlazytraveller]

Steam release tight karna ki loose?

[@gowthamib1766]

Thank you but I have a small doubt how many whistle s came out

[@tusharrajput7850]

Mam gauze piece ko kitane temperature pe sterilize khota hai?

[@julisharkar3315]

Anyone from Shree Janagapati ❤

[@riddlegenius47]

Should we use distilled water in autoclave? or the tap water?

[@solutionpharmacy]

You can use normal water

[@bikashsahoo6539]

More videos about sterilization

[@nicoledelas-alas319]

Can you autoclave lab materials and cultures at the same time?

[@organicnaturallife4479]

Yes but cover the lab material's glass ware with aluminium foil

[@Daytodaytimepass]

how many rubees mam

[@namitadewan3001]

How to know when the article is sterlised. For how much time do we have to to keep the article in this autoclave..

[@solutionpharmacy]

This details are given in standard pharmacopoeia and we have to follow as per object.

[@amitingle6025]

Can we add any disinfectant solution in autoclave

[@solutionpharmacy]

Not required. It may cause staining

[@amitingle6025]

Solution- Pharmacy ok.. thankyou

[@Nikii-qz9xc]

Kon kon mlt se h 😗
Vo like karo

[@shyamagrawal36]

Sir water bath kise kahte hai and isse vaccine ka sterlization hota hai kya

[@solutionpharmacy]

Shyam Agrawal Water bath ek aisa chamber hai jisme pani ko garam karke ek temp par heat kia jata hai. Vaccine ka nahi par iska upyog dusre purpose ke liye kia jata hai

[@sadhnakalra9957]

Kha milega ye mai Faridabad se hu iski jrurt hai mujhe

[@agmpublicschool]

Price kya h

[@techritvik6064]

Kon kon se material ko sterilization ke liye autoclave me

[@solutionpharmacy]

Jo heat aur pressure withstand kar paye

[@techritvik6064]

Ok 🤍

[@sumairaraza8811]

Madam is ko ab start krna bhh bta den

[@SunitaSharma-ew3fl]

Aoutocleve machine Jaipur me khan milti h or Kitne rupees Ki sabse sasti machine Kitne ki h

[@dhananjay5352]

Order karana padta hai..

[@jagannathhalder9822]

Autoclave price kya hai ?

[@solutionpharmacy]

No idea. We are actually not seller

[@user-jk8op4cj8o]

Dmlt walo kya haal hai

[@mahadabdi4158]

English subtitles plz

[@solutionpharmacy]

Video is also in english. Please find

[@solutionpharmacy]

Link given in description box

[@mehtajaydip6884]

Why emulsion are not sterilized in autoclave?

[@solutionpharmacy]

Heat sensitive and phase separation.

[@HindustaniDil-tu8eq]

J

[@himanshu9414]

पका दिया
इसे ऑपरेट कर के बताते.तो अच्छा होगा..
वीडियो थोड़ा छोटा होना चाहिए.
और

[@solutionpharmacy]

Dear sir. This was an demo of parts as mentioned on title. Sorry for the length for you but for a new and avg level student is was required.

[@KiranYadav-qc3cl]

explain in English

[@solutionpharmacy]

Also available in English. Please check in channel

[@DhirajKumar-nj2xi]

Hindi

[@solutionpharmacy]

Pahle se Hindu me bhi hai, please search in channel

[@dora293]

Why you put English 😕

[@solutionpharmacy]

Sorry sir by mistake that was written as we have uploaded same video in English. Now corrected. Thank you for your information. Sorry again

[@JatinderSingh-uk6og]

Bekar and risky

[@solutionpharmacy]

Sorry. It not for playing its peactical. Is druving bike or using mobile is 100% safe ?

[@kiranpal4348]

Thank u mam