Autoclave | Sterilization Process of Glassware and Nutrient Media by Moist Heat Sterilization

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AUTOCLAVE
The autoclave is a container similar to a pressure cooker. It is filled with the objects to be sterilised and then sealed. Next, high-temperature steam is forced in under high pressure, thereby displacing air. Moist heat destroys microorganisms by the irreversible coagulation and denaturation of enzymes and structural proteins. The time and temperature to accomplish this depending on pressure and the type of microorganisms to be inactivated. After the necessary time has passed, the steam is released and the sterilised objects are removed. The entire cycle can take from 15 to 60 minutes (batch processing). pushpendra patel solution pharmacy. vandana janghel solution pharmacy. A more commonly used method when extended heat is not a concern is to use an autoclave or pressure cooker.
PRINCIPLE
The basic principle of steam sterilization, as accomplished in an autoclave, is to expose each item to direct steam contact at the required temperature and pressure for the specified time. Thus, there are four parameters of steam sterilization: steam, pressure, temperature, and time. The two common steam-sterilizing temperatures are 121°C (250°F) and 132°C (270°F).
Moist heat destroys microorganisms by the irreversible coagulation and denaturation of enzymes and structural proteins. In support of this fact, it has been found that the presence of moisture significantly affects the coagulation temperature of proteins and the temperature at which microorganisms are destroyed.
Uses
Steam sterilizers also are used in healthcare facilities to decontaminate microbiological waste and sharps containers. Autoclave is particularly useful for media containing water that cannot be sterilized by dry heat. It is the method of choice for sterilizing the following:
1. Surgical instruments
2. Culture media
3. Autoclavable plastic containers
4. Plastic tubes and pipette tips
5. Solutions and water
6. Biohazardous waste
7. Glassware (autoclave resistible)
PROCEDURE
1. Place the material to be sterilized inside the pressure chamber and fill the cylinder with sufficient water
2. Close the lid and put on the electrical heater.
3. Adjust the safety valve to the required pressure.
4. After the water boils, allow the steam and air mixture to escape through the discharge tap till all the air has been displaced
5. This can be tested by passing the steam-air mixture liberated from the discharge tap into a pail of water through a connecting rubber tube.
6. When the air bubbles stop coming in the pail, it indicates that all the air has been displaced by steam.
7. Close the discharge tap. The steam pressure rises inside and when it reaches the desired set level (e.g. 15 pounds (lbs) per square inch in most cases), the safety valve opens and excess steam escapes out.
8. Count the holding period from this point of time, which is about 15 minutes in most cases.
9. After the holding period, stop the electrical heater and allow the autoclave to cool until the pressure gauge indicates that the pressure inside is equal to the atmospheric pressure. Open the discharge tap slowly and allow the air to enter the autoclave.
10. Open the lid of the autoclave and remove the sterilized materials.
This video will help you to understand- autoclave working, the principle of an autoclave, sterilization process, the importance of sterilization, sterilization process, moist heat sterilization, working of an autoclave, construction of autoclave, how autoclave works,
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[@harshitachourey3373]

Very very useful for me...I saw many videos on TH-cam to understand the autoclave...but no one has made this topic easiy as u did...🥰🥰🥰🥰

[@solutionpharmacy]

Thank you so much for your kind feedback

[@madhusudhanaraja]

Very simple and clearly explained. Thankful for the detail and ease of use

[@Shayarsaqib]

Thank you very much
For Your most Valuable video

[@kumaranand3892]

Very good exlpanation 👌👌
Thanks so much ..

[@nitendra5766]

Thankyou ma'am very informative video
For what I was looking for 😀😀

[@_m.st_09]

Amazing explaination mam 👏👏👏

[@ashupawar1125]

I like ur video mam.... Aapne bde ache trike se btaya sb

[@Mayur_Chandegara]

Thank you for this information 😊

[@Adi-zf5ph]

Good explanation with practicals

[@yaminisahumb1911]

Thank you mam for explanation....🙏

[@saveearth5850]

Awesome explanation ma'am thanks

[@sanidulislam2081]

Thanks a lot for making this video ma'am

[@luckyali4044]

Very good this is video I understand methods of autoclave👍👍

[@surendradwivedi7475]

Bahut sahi tarike se bataya maim ne 💯☝️😊👌👌👌👌👌

[@pharmacy.984]

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[@emory222]

Easiest video ever to understand working of autoclave tnxxx ma'am:)🥰🥰🥰

[@akhilkumardas7904]

Thank you 😊😊 Superb 👍👍👍
This video clear my concept 😊

[@abdulriyaz1336]

Good presentation 👍

[@manand287]

Thanks for your information 🙏

[@shoyabmasuldar2856]

Very Good information about Autoclave, I can clearly understanding Thank You So Much😊

[@shweta.e]

Thank you mam very nice explain 👍👍

[@shabeemtp315]

Gr8, THANKS FOR VIDEO

[@rulakakatipandey9554]

Thank you for useful information

[@kalyani1724]

Best. Video mam..
Thank you❤😍

[@GSMedicalAcademy]

Bahut bahut thanku mam

[@centralsterilesupplydepart9580]

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[@solutionpharmacy]

Thank you

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[@solutionpharmacy]

Pleasure

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Very helpfull video mam🙏

[@txrmafiagaming5310]

Very helpful procedure tq u....

[@ravidhyani1011]

U explained everything be smoothly

[@solutionpharmacy]

Thank you

[@dikshatejan5813]

Bahut badhiya explanation thnk you 👍👍

[@solutionpharmacy]

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[@mohammedsuhaib8514]

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[@9219736563]

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[@pdpharmae-tutor2453]

Excellent Mam

[@khursheednagori8659]

Thank you. 🙂

[@Prajwal.Patil.01]

Great information

[@md.sharifulislamswapon2685]

Best video on autoclaving

[@solutionpharmacy]

Thank you

[@hamjaansari6645]

Good presentation mam

[@LofispotA]

Thanku

[@muskansingh689]

Well explained thanku mam

[@abhishekmogal1933]

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[@deepak03kir]

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[@archanaarchu2399]

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[@solutionpharmacy]

Thank you

[@stockwalasw]

Thanks ma'am

[@rameshthuniki7469]

Mam your explanation is very clear..thank you mam

[@dheerajtiwari920]

Very clearly explanation...Thank you mam🙏🙏

[@haseebjutt9917]

Smjh aye ha

[@rubikushwah8931]

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Thanku so much

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[@solutionpharmacy]

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[@learnchemistry8817]

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[@mr.pushapdeep988]

Thank god for making this video

[@solutionpharmacy]

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[@alkaahirwar4103]

Very nice video and very helpful thx

[@solutionpharmacy]

My pleasure

[@indian__nature97]

nyc information..thnkiu

[@Ramakantmumbai]

Nicely explained. Thanks. I need to sterilize a metallic object duly sealed in a pouch. Which pouch I can use for this purpose

[@hannahbaker6105]

😂

[@iffatafreen2206]

Thank you mam❤️

[@hosenurrahmanmollick5252]

Thanks a lot ma'am

[@suranderthakur9012]

Thanku you

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Helpful👆👍

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thanx..........nice explanation

[@solutionpharmacy]

gopal player Thank you

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Thanku very much mam🙏

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Thank You

[@mesramshivaprasad6802]

Thanks mom

[@TheDreamBird11]

Thanks for the video 😊

[@solutionpharmacy]

My pleasure

[@dattaprabhuandhale7608]

Thanks mam ❤️👍🙏

[@Amitkumar-eu3hl]

Bahut khub maim

[@jaanbhaipankaj]

Thank you

[@amittripathi5068]

Thanks mam.

[@akankshasingh4706]

Thnku mam

[@chhandadas3251]

Thank you mam for nicely explain 😊

[@alipatulavi1359]

Thnx mam

[@ErJagroopSingh]

We also need to cover the cotton plug (using foil paper, etc) as well, unless it well moist.

[@latadonode1051]

Se

[@mdmaaz4987]

Tujhe bohat mlm hai

[@supreethayadav5585]

Sprrr tqqqq very much

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Thank you

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Good

[@sugyanibehera9812]

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[@solutionpharmacy]

Thanks 🤗

[@lifeisjournysoulispeaceful8826]

tq so much

[@simon1793]

Mem hitting joint may lik hoya kiya souliotion hai? Same autoclave.

[@user-hd5rm4hc9b]

Thanks for explaining ❤️❤️ can you answer me why we cover baeker and petridish??

[@solutionpharmacy]

To prevent from salt depisition

[@minkikumari2917]

Incubator chalane,knowledge

[@ravikanaujiya6372]

Thank u mem🙏

[@ajmersaroha6103]

Awesome

[@madhvisharma3149]

Good translation mam.

[@solutionpharmacy]

Thank you

[@monikasharma7959]

Thankyou mam

[@Suzaaneducation]

mam 15 lbs k upar pressure jane k baad whistle apne aap bajne lagta hai ya use manual karna padta hai please reply

[@r.naganathdavey6837]

Thanks for the video in my auto clave i have one more option "vaccum release valve" can u pls tell me what is tge procedure for that

[@solutionpharmacy]

R.Naganath Davey That is used for releasing a small amount of vapour so that vaccume may not created.

[@ravindarverma855]

Which make you have autoclave

[@itishreepriyadarsaninayak3341]

Thank u mam

[@pujathakurathi9415]

Thanks

[@solutionpharmacy]

My pleasure

[@varshasadhwani1452]

Please help me incubator par vedio bna dijiye

[@freehajavaid636]

Pls tell me how to maintain 15psi temp?

[@DeepakThakur-cf9vu]

Mam thanks

[@jeevanpandey4057]

MAM
STERILISED SURGICAL DRUMS KE INSTRUMENTS (AUTOCLAVED) KO HUMLOG KITNE DINO BAD TAK PRAYOG ME LA SAKTE HAIN.

[@rojangaikwad3879]

Mam conical flask ko wrap Nahi kiya paper se

[@faizkhokhar2302]

Thankyou sooo much mam bookage language mai maine kafi baar pdhaaa magar smjh nhi aa raha tha but after watching your video ek hi baar mai procedure smjh mai aa gya 😅

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Aap kyankrte ho

[@mahendradewasi6795]

Tq