Your explanations are so clear - I feel pleased yet highly concentrated while listening to your lectures. I think I'm getting more and more interested in biochemistry - even though I've been studying it for 5 weeks only...🤣 I was afraid I would lose the chance to understand TCA and pyruvate decarboxylation thing and so on - but you gave me hope (and knowledge). Thank you so much, you're unbelievably cool!
Thanks for the video. There's something I don't get about step 4 though. In the step that converts alpha-ketoglutarate into succinyl-coA an NAD+ gets reduced into NADH. In about 50% of the sources I have found, there is also an H+ represented along with the newly produced NADH. Where does it come from and why is it only represented half of the time? I can understand where the new H on NADH comes because there is an H in HS-coA, but I don't know about the second H.
The alpha-ketoglutarate dehydrogenase complex is not similar to the enzyme in step one of the citric acid cycle, instead it should bepyruvate dehydrogenase complex used in the preparation of acetyl-CoA, I think.
For those reading in the future, he isn't saying the two enzymes are similar but the way the two substrates in interact with the enzyme (with CoA involved in both steps) is similar. A CoA-S is attached in both cases
7:10 Step 2 of the reaction catalyzed by Isocitrate Dehydrogenase (step 3 of the TCA cycle) is not the rate determining step according to Biochemistry by Stryer, its the next step, catalyzed by alpha-ketoglutarate dehydrogenase.
I'm no expert, but according to the NCBI and four other places I checked, the formation of the alpha-ketoglutarate from isocitrate using isocitrate dehydrogenase (step 3) is the rate determining step. He is saying that 1 and 2 of step 3 are the rate determining step
He meant the video about the Oxidative Dercarboxilation of the Piruvate, not the Step 1. It's the Piruvate Dehydrogenase Complex that is similar to the Alfa-Ketoglutarate Dehydrogenase Complex.
You're the best and I am so happy I found your channel. I wouldn't have been able to get through Biochem this summer without you!
Your explanations are so clear - I feel pleased yet highly concentrated while listening to your lectures. I think I'm getting more and more interested in biochemistry - even though I've been studying it for 5 weeks only...🤣 I was afraid I would lose the chance to understand TCA and pyruvate decarboxylation thing and so on - but you gave me hope (and knowledge). Thank you so much, you're unbelievably cool!
is there a way of getting the notes on the whiteboard? would love to use them for revision!!
Thanks for the video. There's something I don't get about step 4 though. In the step that converts alpha-ketoglutarate into succinyl-coA an NAD+ gets reduced into NADH. In about 50% of the sources I have found, there is also an H+ represented along with the newly produced NADH. Where does it come from and why is it only represented half of the time? I can understand where the new H on NADH comes because there is an H in HS-coA, but I don't know about the second H.
The alpha-ketoglutarate dehydrogenase complex is not similar to the enzyme in step one of the citric acid cycle, instead it should bepyruvate dehydrogenase complex used in the preparation of acetyl-CoA, I think.
what i was about to say... there are more small mistakes ( ex. organization of molecule )
For those reading in the future, he isn't saying the two enzymes are similar but the way the two substrates in interact with the enzyme (with CoA involved in both steps) is similar. A CoA-S is attached in both cases
that is what he meant by step one, he's talking about the pyruvate dehydrogenase complex
7:10
Step 2 of the reaction catalyzed by Isocitrate Dehydrogenase (step 3 of the TCA cycle) is not the rate determining step according to Biochemistry by Stryer, its the next step, catalyzed by alpha-ketoglutarate dehydrogenase.
I'm no expert, but according to the NCBI and four other places I checked, the formation of the alpha-ketoglutarate from isocitrate using isocitrate dehydrogenase (step 3) is the rate determining step. He is saying that 1 and 2 of step 3 are the rate determining step
He meant the video about the Oxidative Dercarboxilation of the Piruvate, not the Step 1. It's the Piruvate Dehydrogenase Complex that is similar to the Alfa-Ketoglutarate Dehydrogenase Complex.
thank you very much!#!!!your explanation is absolutely clear and i wish you are my lecturer:(