Electrophoretic Mobility Shift Assay | EMSA | Gel Retardation Assay | Gel Shift Assay | PENS#91

Electrophoretic Mobility Shift Assay | EMSA | Gel Retardation Assay | Gel Shift Assay | PENS#91
#EMSA #gelretardation #proteinDNAinteractions
An electrophoretic mobility shift assay (EMSA) or mobility shift electrophoresis is a typical affinity electrophoresis technique used to analyse protein-DNA or protein-RNA interactions. It is also known as a gel shift assay, gel mobility shift assay, band shift assay, or gel retardation test. This process can tell you if a protein or a mixture of proteins can bind to a specific DNA or RNA sequence, and it can also tell you if the binding complex contains more than one protein molecule. When researching transcription initiation, DNA gang replication, DNA repair, or RNA processing and maturation, as well as pre-mRNA splicing, gel shift assays are frequently used in vitro in conjunction with DNase footprinting, primer extension, and promoter-probe investigations.
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The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) New Delhi. Dr. Singh has a vast experience of research and Teaching in the fields of Structure & Molecular Biology and Biochemistry, which also include Biomolecules, Proteomics, Protein Engineering and Drug Design.
Dr. Nagendra Singh currently works at the School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA (www.gbu.ac.in/FacultyProfiles/.... He does research in Molecular Biology, Structural Biology and Biotechnology. His current research interest include 'Structural and functional characterization of proteins involved in RNA metabolism from human and microbial origin. Dr Singh has published over 70 research articles in intentional Journals of good repute. He has over 20 awards and honors on his name. Few of the awards include "National Academy of Sciences of India (NASI, Allahabad) young scientist award", UGC-NET, CSIR-JRF, GATE, Best Research Awards etc. Dr. Singh has participated and presented research work at over 50 national and International conferences world wide.

[@simransharma4246]

Great Explanation :)

[@proteinengineering007]

I m glad it helped u @Simran Sharma