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Protein Engineering
India
เข้าร่วมเมื่อ 30 พ.ย. 2015
The Lectures are given by Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions along with science opinions on contemporary issues. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) New Delhi. Dr. Singh has a vast experience of research and Teaching in the fields of Structure & Molecular Biology and Biochemistry, which also include Biomolecules, Proteomics, Protein Engineering and Drug Design. Dr. Singh has published over 70 research articles in international journals and carry over 20 awards and honors on his name.
Dr. Nagendra Singh currently works at the School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA (www.gbu.ac.in). These lectures will also aid preparation of competitive examinations such as UGC NET | CSIR JRF | DBT JRF | ICMR JRF etc. for aspirants.
Dr. Nagendra Singh currently works at the School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA (www.gbu.ac.in). These lectures will also aid preparation of competitive examinations such as UGC NET | CSIR JRF | DBT JRF | ICMR JRF etc. for aspirants.
Unlocking the Secrets of ICSBDD-2023 (3) | Classical Dance Performance by Sirisha | PENS#100
#ICSBDD-2023 # danceperformance #GBU
International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA. My students performed in the cultural program to showcase their extracurricular talent. These are all UG/PG students who worked tirelessly to make ICSBDD a grand success. as a token of appreciation, this video is uploaded on PENS.
International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA. My students performed in the cultural program to showcase their extracurricular talent. These are all UG/PG students who worked tirelessly to make ICSBDD a grand success. as a token of appreciation, this video is uploaded on PENS.
มุมมอง: 211
วีดีโอ
Unlocking the Secrets of ICSBDD-2023 (1) | Dance Performance | Retro to Metro| PENS#98
มุมมอง 5118 หลายเดือนก่อน
#ICSBDD-2023 # danceperformance #GBU International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA. My students performed in the cultural program to showcase their extracurricular talent. These are all UG/PG students who worked tirelessly to make ICSBDD a grand...
International Conference on Structural Biology & Drug Discovery-2023| ICSBDD-2023 | PENS#97
มุมมอง 868 หลายเดือนก่อน
#ICSBDD-2023 #GBU International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA.
NMR Spectroscopy (Part 4) | Proton Exchange | Deuterium in H1 NMR | Dr. Nagendra Singh | PENS#96
มุมมอง 915ปีที่แล้ว
NMR Spectroscopy (Part4) | Proton Exchange | Deuterium in H1 NMR | Dr. Nagendra Singh | PENS#96 #nmrspectroscopy #protonexchange #nmrspectra The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) Ne...
NMR Spectroscopy (Part 3) | Mechanism of Signal Splitting in NMR | Dr. Nagendra Singh | PENS#95
มุมมอง 562ปีที่แล้ว
NMR Spectroscopy (Part 3) | Mechanism of Signal Splitting in NMR | Dr. Nagendra Singh | PENS#950 #nmrspectra #peaksplitting #nmrspectroscopy The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) Ne...
NMR Spectroscopy (Part 2) | NMR Spectra | Chemical Shift || Dr. Nagendra Singh | PENS#94
มุมมอง 743ปีที่แล้ว
NMR Spectroscopy (Part 2) | NMR Spectra | Chemical Shift || Dr. Nagendra Singh | PENS#94 #nmrspectroscopy #nmrspectra #chemicalshift NMR spectroscopy is the use of NMR phenomena to study the physical, chemical, and biological properties of matter. Chemists use it to determine molecular identity and structure. Medical practitioners employ magnetic resonance imaging (MRI), a multidimensional NMR ...
NMR Spectroscopy (Part 1) | Basic Principle | H1 NMR | Dr. Nagendra Singh | PENS#93
มุมมอง 1.2Kปีที่แล้ว
NMR Spectroscopy (Part 1) | Basic Principle | H1 NMR | Dr. Nagendra Singh | PENS#93 #nmrspectroscopy #nmr #nmrspectra NMR spectroscopy is the use of NMR phenomena to study the physical, chemical, and biological properties of matter. Chemists use it to determine molecular identity and structure. Medical practitioners employ magnetic resonance imaging (MRI), a multidimensional NMR imaging techniq...
GST Pull Down Assay & Co Immunoprecipitation | Protein-Protein Interactions | PENS#92
มุมมอง 4.3K2 ปีที่แล้ว
GST Pull Down Assay & Co Immunoprecipitation | Protein-Protein Interactions | PENS#92 The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) New Delhi. Dr. Singh has a vast experience of research an...
Electrophoretic Mobility Shift Assay | EMSA | Gel Retardation Assay | Gel Shift Assay | PENS#91
มุมมอง 1.3K2 ปีที่แล้ว
Electrophoretic Mobility Shift Assay | EMSA | Gel Retardation Assay | Gel Shift Assay | PENS#91 #EMSA #gelretardation #proteinDNAinteractions An electrophoretic mobility shift assay (EMSA) or mobility shift electrophoresis is a typical affinity electrophoresis technique used to analyse protein-DNA or protein-RNA interactions. It is also known as a gel shift assay, gel mobility shift assay, band...
Peptide Mass Fingerprinting | Protein Fingerprinting | PMF | Protein Identification | PENS#90
มุมมอง 3.2K2 ปีที่แล้ว
#peptidemassfingerprinting #PMF #proteinidentification Peptide Mass Fingerprinting | Protein Fingerprinting | PMF | Protein Identification | PENS#90 Peptide mass fingerprinting (PMF) (also known as protein fingerprinting) is an analytical technique for identifying proteins by first cleaving an unknown protein of interest into smaller peptides and then mass spectrometrically analyzing them, such...
2D Gel Electrophoresis| Principle & Limitations | 2 Dimensional Gel Electrophoresis | 2DGE | PENS#89
มุมมอง 2.4K2 ปีที่แล้ว
2D Gel Electrophoresis| Principle & Limitations | 2 Dimensional Gel Electrophoresis | 2DGE | PENS#89 #2dgel #2DGE #proteomics The primary technique for proteomics research is two-dimensional gel electrophoresis, or 2D-PAGE. It uses two different features of proteins to distinguish the complicated mixture of samples. Proteins are distinguished in the first dimension by their pI value, and in the...
Introduction to Proteomics | Proteomics and Genomics | Aims and Objectives of Proteomics | PENS#88
มุมมอง 1.8K2 ปีที่แล้ว
Introduction to Proteomics | Proteomics and Genomics | Aims and Objectives of Proteomics | PENS#88 #proteomics #genomics #proteome Proteomics is the study of a cell's, tissue's, or organism's total protein complement under a set of defined conditions. It is currently reliant on decades of technological and instrumental advancements. Advances in mass spectrometry (MS) technology, protein fractio...
Tandem Affinity Purification (TAP) | Protein-Protein Interactions | Techniques | PENS#87
มุมมอง 5K2 ปีที่แล้ว
#tandemaffinitypurification #tap #proteomics Tandem Affinity Purification (TAP) | Protein-Protein Interactions | Techniques | PENS#87 The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) New Delhi...
Protein - Protein Interactions | PPI | Techniques | Dr. Nagendra Singh | PENS#86
มุมมอง 2.7K2 ปีที่แล้ว
#proteinproteininteractions #proteomics #oligomericproteins Protein - Protein Interactions | PPI | Techniques | Dr. Nagendra Singh | PENS#86 protein protein interaction techniques protein protein interaction examples protein protein interaction analysis protein protein interaction assay of protein-protein interaction importance of protein protein interaction application of protein-protein inter...
Sequential and Ping Pong Reactions Mechanism | Bi-Substrate Enzyme Reactions | PENS#85
มุมมอง 4.8K2 ปีที่แล้ว
Sequential and Ping Pong Reactions Mechanism | Bi-Substrate Enzyme Reactions | PENS#85
Edman Degradation (Part 2) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#84
มุมมอง 7292 ปีที่แล้ว
Edman Degradation (Part 2) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#84
Edman Degradation (Part 1) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#83
มุมมอง 1.4K2 ปีที่แล้ว
Edman Degradation (Part 1) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#83
Alpha Helix | Protein Structure | Numerical Questions | PENS#82
มุมมอง 9032 ปีที่แล้ว
Alpha Helix | Protein Structure | Numerical Questions | PENS#82
UV Visible Spectroscopy (Hindi) | Spectrophotometer | Empirical Rules| Dr. Nagendra Singh | PENS#81
มุมมอง 8822 ปีที่แล้ว
UV Visible Spectroscopy (Hindi) | Spectrophotometer | Empirical Rules| Dr. Nagendra Singh | PENS#81
Mole Concept (PART 2) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#80
มุมมอง 6342 ปีที่แล้ว
Mole Concept (PART 2) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#80
Mole Concept (PART 1) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#79
มุมมอง 9672 ปีที่แล้ว
Mole Concept (PART 1) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#79
Easy ways of Online Teaching | Teaching with PPT and PDF | Dr. Nagendra Singh | PENS#78
มุมมอง 2083 ปีที่แล้ว
Easy ways of Online Teaching | Teaching with PPT and PDF | Dr. Nagendra Singh | PENS#78
How to Make an Effective Power Point Presentation (Hindi) | PPT Rules | Dr. Nagendra Singh | PENS#77
มุมมอง 6983 ปีที่แล้ว
How to Make an Effective Power Point Presentation (Hindi) | PPT Rules | Dr. Nagendra Singh | PENS#77
Part Per Million (PPM) | PPB | PPT| Numerical Questions | Dr. Nagendra Singh | PENS#76
มุมมอง 8223 ปีที่แล้ว
Part Per Million (PPM) | PPB | PPT| Numerical Questions | Dr. Nagendra Singh | PENS#76
ESI-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry | Dr. Nagendra Singh | PENS#75
มุมมอง 3.8K3 ปีที่แล้ว
ESI-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry | Dr. Nagendra Singh | PENS#75
MALDI TOF Mass Spectrometry | Deconvolution of Mass Spectra | Dr. Nagendra Singh | PENS#74
มุมมอง 11K3 ปีที่แล้ว
MALDI TOF Mass Spectrometry | Deconvolution of Mass Spectra | Dr. Nagendra Singh | PENS#74
Ion Detectors in Mass Spectrometry | Faraday Cup| Electron Multipliers| Dr. Nagendra Singh | PENS#73
มุมมอง 14K3 ปีที่แล้ว
Ion Detectors in Mass Spectrometry | Faraday Cup| Electron Multipliers| Dr. Nagendra Singh | PENS#73
UV Vis Spectroscopy | Principle and Applications | Empirical Rules | Structural studies | PENS#81
มุมมอง 9723 ปีที่แล้ว
UV Vis Spectroscopy | Principle and Applications | Empirical Rules | Structural studies | PENS#81
Best COVID-19 Vaccine | Mechanism of Action | COVID 19 Vaccine Design | Dr. Nagendra Singh | PENS#72
มุมมอง 4823 ปีที่แล้ว
Best COVID-19 Vaccine | Mechanism of Action | COVID 19 Vaccine Design | Dr. Nagendra Singh | PENS#72
For 5 sample test tube, instead of water for volume make upto 1000micorliter which buffer should we add
one can use same buffer in which protein sample is dissolved.
Which diluent add in bradford reagent to dilute upto 1L
water is added to make up the volume.
Thank you so much
Are sirji lekin job hi nahi milti to kya fayda biotechnology kar ke
Very informatic vidio we got all required knowledge on bio technology thanks a lot.
Thanks sir bohat achy se samjha ya aap ny
How BSA concentration(mg/ml) of test tube 1-5 is calculated?
2024 𝚖𝚎 𝚔𝚎𝚢𝚊 𝚑𝚎
Big thanks for explaining the topic in such easy manner 🫡
I am glad @harshitapandey8874 ..thank you
Very well explained 🎉
Sir msc biotech kr lene ke turant baad achi job milti h ya nhi.
In india scope h ya nhi
In india scope h ya nhi
Thank you sir it was very insightful.
Thank you so much sir 🙂
Nice information.
Very nice sir . I satisfied of your video thx 🙏🙏🙏🙏🙏🙏🙏 sir
thanks for very useful content👌👍🙏
Sir, at 12:29, the first amino acid will superimpose exactly on the 19th amino acid and not 18th, according to the calculation.
Vry.nicely explained with detail Guidence related to Biotechnology.Thanks for elaborate Awerness.
Gracefully performed ❤
👌👌❤
Super chala chala bagundi
చాలా బాగా చేసింది
Very beautiful
Much helpful lecture sir, thanks a ton
Thank you so much Sir, very well explained in easy words .
i m glad..thank u
U all made the conference a huge success...thanks to all of you for such a wonderful and entertaining contribution...❤
🎉🎉🎉🎉🎉 too good
Indeed, a treat to eyes. 😍
Unforgettable moment ✨💕 Everything is just so perfect.
Thank you much informative
thank you
Excellent explanation - not wordy, to the point with excellent relevant examples throughout; clear and concise! Thank you Dr Singh! 👍
Thank you
C g goverment school me lekcharara ban sakte hai ya nahi
yes
Thank you for your informative video. Just I think the calculation needs some correction. If u take 0.2 ml from 1mg/ml stock solution and make 1 ml standard solution, the concentration will be 200 microliter/ml, not 20 microliter/ml. The same for the following conc
On what basis we use different detectors
depending on nature of analyte and cost available.
File ko mobile me kese send kre
connect laptop to PC and transfer ppt file as such
Sir file save kese kre please btaiye
file apne app save ho jayegi..presentation end hone ke bad
Sir i have done bsc biotechnology honours can i be pgt biology in madhya pradesh?
Biotechnology fields are the varry interesting fild
Kindly reply me sir..is sodium potassium tartrate and potassium sodium tartrate same?? Because I have potassium sodium tartrate.
yes is is obvious....
😊
V informative
Sir MSc biotechnology m krne k bad private ya govt hospital m work kr skate h kyaa lab m
yes
Thank thank u so much sir very useful video
Well presented and explained , thank you!
I am glad it helped.. @Anun Pandit
Khane k bad jana hota ye prb bola doctor weight badhana h
How to overcome
Pls can any one summarise in English...
Thank you sir for this video 🙏🙏🙏
I have been always looking for this kind of information, finally got it. Thank you very much. Kindly share more and detail videos regarding this.
This is the best description of mass spec detectors I've been able to fine. Thank you for taking the time to put it together.
@Emily Nischwitz ... I am glad it helped ...Thank you
Thank you so much for this insightful and detailed video on 2DGE. May I ask you two questions? 1. What is the chemistry behind the formation of the pH gradient in the IPG strips? 2. As SDS completely disrupt the polymeric configuration of a protein, doesn't it affect further downstream analysis like MS methods? It will be helpful if you please answer this.
@Aditi Sarkar ... I will be glad to answer the queries. 1. Buffers are nothing more than a mixture of acid-basic groups. on IPG strips, amphoteric groups are covalently immobilized on a matrix such as polyacylamide to create their concentration gradient. 2. SDS disrupt all structure of proteins except the primary structure (sequence). so after SDS PAGE only sequence of a protein remains, that is what required to identify and further characterization by MS. MS identifies protein by either sequencing in MS/MS or by peptide mass finger printing (PMF). MS does not provide any details about function of 3d structure. so it doesnt matter to MS if protein has lost tertiary or quaternary structure. I hope it will help u in further understanding. Feel free if any doubt persists.
@@proteinengineering007 Okay! Thank You, Sir. Take homage🙏