PRODUCING DNA FRAGMENTS- Methods to produce DNA fragments for A-level recombinant DNA technology

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  • เผยแพร่เมื่อ 18 ธ.ค. 2024

ความคิดเห็น • 48

  • @keke3897
    @keke3897 4 ปีที่แล้ว +22

    when do you plan to have the other topic 8 videos up? you're the only teacher that makes it so simple

    • @MissEstruchBiology
      @MissEstruchBiology  4 ปีที่แล้ว +5

      I should have the topic 8 epigenetics video uploaded by tomorrow.
      I'm hoping to have the in vivo cloning videos up from Monday.
      Sorry for the delay, I have been ill with no voice since Tuesday, so have been unable to record any audio for the videos!
      Really please that you find the videos easy to follow :)

    • @MissEstruchBiology
      @MissEstruchBiology  4 ปีที่แล้ว +9

      Hello, just to let you know the in vivo cloning videos are up now :)

  • @leahlester2620
    @leahlester2620 4 ปีที่แล้ว +10

    Wow that was quick! Thank you

    • @MissEstruchBiology
      @MissEstruchBiology  4 ปีที่แล้ว +1

      It's not the epigenetics video yet (acetylation and methylation), but is still prat of topic 8 AQA biology

  • @mariyamjoona679
    @mariyamjoona679 3 ปีที่แล้ว +43

    Who is the one disliking these videos man?🥺these are so helpful🥰

    • @MissEstruchBiology
      @MissEstruchBiology  3 ปีที่แล้ว +7

      haha awww thanks.
      So glad you find them so helpful :D

  • @unicorn1025
    @unicorn1025 3 ปีที่แล้ว +5

    great content!

  • @kamiliab3938
    @kamiliab3938 10 หลายเดือนก่อน

    Thank you so much 🙏🙏

  • @riihanna510
    @riihanna510 6 หลายเดือนก่อน +12

    this is genuinely the worst topic in this subject, but you've made it easy! so nervous for paper 2 tho

    • @MissEstruchBiology
      @MissEstruchBiology  6 หลายเดือนก่อน +2

      good luck!

    • @raegardens8339
      @raegardens8339 6 หลายเดือนก่อน +3

      Good luck! I think most of us are in the same place

    • @riihanna510
      @riihanna510 6 หลายเดือนก่อน +2

      @@raegardens8339 yeah i’m trying to finish genome projects and technology but it’s so boring that i can’t😭😭😭 wishing you the best of luck too❤️❤️

  • @uwotm8770
    @uwotm8770 2 ปีที่แล้ว +1

    hello miss, what does it mean by 'overlapping nucleotides' ?

    • @adailyfact
      @adailyfact 10 หลายเดือนก่อน

      What grade did you get?

    • @uwotm8770
      @uwotm8770 10 หลายเดือนก่อน +1

      @@adailyfact grade A and 8 marks from A*

    • @adailyfact
      @adailyfact 10 หลายเดือนก่อน +1

      @@uwotm8770 well done! What are you studying now? Any tips?

  • @sanjanamehrotra743
    @sanjanamehrotra743 3 ปีที่แล้ว +2

    What are introns in simple words?

    • @MissEstruchBiology
      @MissEstruchBiology  3 ปีที่แล้ว +6

      hello,
      They are sections of DNA that don't code for amino acids. So it is particular sequences of DNA that don't code for amino acids

    • @Jefrryy
      @Jefrryy 9 หลายเดือนก่อน

      Non coding sections of mRNA

  • @Mozzie7920
    @Mozzie7920 3 ปีที่แล้ว

    3:10 I don’t understand the first part of the process, literally just the first point

    • @rowndhhhhhh
      @rowndhhhhhh 8 หลายเดือนก่อน +1

      what grade did you end up getting?

  • @stephanie4548
    @stephanie4548 ปีที่แล้ว +1

    would we need to know the stages of how DNA fragments are produced in detail?

    • @student8122
      @student8122 9 หลายเดือนก่อน +1

      yes

  • @ostric8768
    @ostric8768 3 ปีที่แล้ว +8

    queen

  • @weevr4259
    @weevr4259 6 หลายเดือนก่อน +37

    I’m so cooked

    • @mjhxrdy
      @mjhxrdy 6 หลายเดือนก่อน

      Real

    • @octobusse3180
      @octobusse3180 6 หลายเดือนก่อน

      Real 💀💀💀

    • @a3lexa
      @a3lexa 6 หลายเดือนก่อน

      fr

    • @Nabuwetunabuwetuissisikuyai
      @Nabuwetunabuwetuissisikuyai 6 หลายเดือนก่อน +3

      Dont worry ill lower d grade boundaries

    • @Mmusa409
      @Mmusa409 6 หลายเดือนก่อน

      Same

  • @catherinesimm3804
    @catherinesimm3804 3 ปีที่แล้ว +6

    I still don’t understand I think I’m just thick tho lol

    • @MissEstruchBiology
      @MissEstruchBiology  3 ปีที่แล้ว +9

      Hi Catherine,
      Gene technology is a challenging topic and many students have to spend time to fully understand it, so you are definitely not thick!
      Is there a particular part that didn't make sense, or any questions you have to see if I can clarify?

    • @qasimkhan34
      @qasimkhan34 9 หลายเดือนก่อน +5

      i understand and im thicc

    • @UbaydullahJarir
      @UbaydullahJarir 9 หลายเดือนก่อน

      @@qasimkhan34 chupsha

    • @Huaa123
      @Huaa123 8 หลายเดือนก่อน +2

      @@qasimkhan34not thicker then me

  • @abz7800
    @abz7800 6 หลายเดือนก่อน

    Anyone who says this makes sense clearly didn't understand what was being taught or the lack of it . We are trying to isolate a particular gene and excluding the missing fact that the mRna needs to be removed from the cdna before dna polyermerase can be used the reform the required gene. There seems to be absolutely zero explination on how he endonucleause is isolating the required gene . We're in a first world country and the teaching seems soo poor came here to since the Oxford textbook is horrid but my understanding hasn't changed after watching this

    • @MissEstruchBiology
      @MissEstruchBiology  6 หลายเดือนก่อน +3

      This is the level of detail required for A-level. Anything beyond that would be university standard

    • @abz7800
      @abz7800 6 หลายเดือนก่อน

      @@MissEstruchBiology I figured it out by looking at some diagrams now . The endonuclease can be used twice at both ends of the required gene to seperate it from the longer dna strand . Also we do actually need to know that the mRNA and cDNA need to be seperated otherwise it doesn’t make sense how is DNA polyermerase used to reform the gene when it only works by recombining adjacent dna nucleotide strands , it’s also mentioned in the textbook that’s where I learnt it .

    • @porcupineou
      @porcupineou 6 หลายเดือนก่อน

      @@abz7800maybe youre using a different example board because the detail included in the video is quite literally the level needed for AQA a level, anything else is extra learning and not strictly required, check the exam board of your book, because the AQA biology books and revision guides include this amount of detail, and other things youve mentioned like it working on both ends to separate it from the dna strand can honestly be assumed using common sense, her video is alright and if its worked for everyone else its because its good

    • @Tommytwometres
      @Tommytwometres 6 หลายเดือนก่อน +1

      @@abz7800Don’t always go off them textbooks they aren’t always accurate. Look at the spec that’s the important stuff. Other places say the mRNA is destroyed “by an enzyme” such as uplearn, but don’t name it as we do not need to know this enzyme at a level. They only do this to make sense. We don’t need to know that. We need to know what miss said here though. She’s said all relevant information you need, it might not make sense but sometimes although stuff doesn’t make sense to you, just have to memorise it!

    • @riihanna510
      @riihanna510 6 หลายเดือนก่อน +1

      uplearn explains the mRNA thing, basically an additional enzyme destroys the original mRNA, but it’s not on spec