Pasteurization vs Sterilization Experiment (Part 1)
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In the previous video I talked about why my manure substrate seems to keep contaminating with trichoderma. Well, someone had posted a comment on that video stating that sterilization works much better than pasteurization for nutritious substrates, which is the complete opposite of what the mainstream understanding is. But who knows? I've never tried so I thought this would make an interesting experiment for you all to see what truly is. I doubt this will work but I hope I'm wrong!
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Taco Bell will do that too
👌My dude. Appreciate the time you take to convey thousands of hours of experience / knowledge in a cogent video format.
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Always enjoying your content!
Sage!!! Thanks for loving mycology bro. I love your no fluff style . And bro you have a voice for radio! It’s so smooth! Lol
Thanks Brandon!
Can’t wait to see the results
Hey what's up bro! I've been so busy with everything here, my apologies for not filling you in, however...I just noticed lol so that Colloidal Silver Tek I was experimenting with..... amazing!!! I covered my plates in CS and actually forgot about them during my move into the new place, I pulled them out 2dys ago expecting catastrophe, it was quite the opposite actually... The 2 plates I treated and didn't touch again were completely clean! I actually lost a whole spore syringe of my top producer, but when I pulled out the CS test plates and saw how thick the ryzomorphic growth had become and how healthy it looked I about dropped the plate lol. I cut 8 specimens from each plate transferring to agar. They exploded almost overnight. So I can say with confidence that my CS Tek will work. CS will destroy any bacteria with single celled walls, the mycelium isn't bothered because it has thick double celled walls. I'm going to up the ante and put CS into the water I use for grain spawn and everything else and see how that works. I'm willing to bet that if one were to add CS to their Agar recipe it would manage contamination phenomenally. Ok I'm getting back to work it's been rough lol.
Hey THC! I’ve just been thinking about messaging you! Great to hear that the CS is working great. I’m curious to see how it would fair against dirty swabs and outdoor spores. Great to see you back here!
@@Mycophilia same I think it'd be a good idea to isolate individual contaminates onto agar and test each one with the CS. It's relatively inexpensive and I'm using a 10ppm solution, they make it up to 500ppm. Imagine soaking your grane spawn in a CS solution and eliminating contam on almost every front. If this continues to prove fruitful the implications would be incredible, open air work stations, no LFHs or SABs. It's good to be back too! Glad to see things are still rolling for you. I hope TH-cam fixes your channel. With several states and municipalities legalization it shouldn't be a problem anymore. I'll keep my fingers crossed. NAMASTE 🙏 I'll hit you up again shortly. Reach out anytime you'd like.
@@treehouseconstituents6402 thanks THC! 🍄 ❤️
@@treehouseconstituents6402I would love to hear an update on this 😮
I don't understand why people believe the 'bad' organisms are killed but 'good' are preserved in pasteurization. Is there science to support that?
Yes, of course.
nothing that would be known on Wikipedia. As far as it is explained there pasteurization simply does reduce the bacterial load where sterilization brings it to (near) zero. As far as I can tell heat and pressure don't distinguish between "good" and "bad" bacteria. They kill microorganisms according to their relative resistance to heat / pressure.
And what's beneficial anyway? perhaps a "low" bacterial or yeats load is. or perhaps some specific strands are beneficial. I haven't seen any hard science on it so far.
@@5.dot.connectorso-called beneficial bacterial in this case is termophilic bacterial (im not sure of this is correct english termin, but they are love heat and wher it Is too hot for others they are thrive). And this becterias can live in substrat and give it some contaminat resistant. But what is more important - they will not mess with our mushrooms. Somehow they are not competing for the substrat
@@Mycophiliacan we see it?
I’m sorry but no the science does not support this. It kills all migroorganisms which are not adapted to high heat. High heat resistant traits include endospore formation, heat resistant protein structures, specialized phospholipid bilayer which has fatty acid tails with higher boiling points etc. now the important part. Both “good” and. “Bad” bacteria are known to have any of these traits. Most good bacteria will die, and a Few will be resistant. Most bad bacteria will die, and a Few will be resistant. Now sterilization all of these traits don’t matter, it’s so harsh it destroys even the most resilient organisms good and bad. Hope this helped😀😀
Interesting video. I stumbled on your channel while searching, I enjoy watching your videos where you breake everything down in simple easy to understand terms. I also like how you dont use reddit for go to answers.... I will definetly be following this series.
I am new to this and was thinking about composting my chicken, goat, and duck poo for using in some trials. I believe it takes a while to compost it down to reduce the nitrogen, and also balance the PH which is some what critical on many levels for using to add to substrates.
I can not wait to see how your journey goes. Thanks for your great videos....
Thank you for your comment, glad to have you here!
As always great content my friend . Tried to email the link in the description and it said invalid ??you will laugh my tubs are listening to your channel .
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@@Mycophilia nope tried and it said it either does not exist or cannot except
@@jennifererin6808 That's very strange, here's my backup email: mycophilesage@gmail.com
i had questions about pasteurization v sterilization but for straw, and it is great seeing your thoughts on a similar matter- & like urself i may have to just fuck around and find out through observation and experiments to get the answer i want
Thank you for confirming my bias. I don't own a pressure cooker and i really don't wanna buy one.
@@Torturantula you’ve got it all wrong. You need a PC if you want to create spawn. You can get away with steaming if using brown rice, but that is still sterilization, not pasteurization. You cannot pasteurize grains.
I found a solution to your problem. Hardwood sawdust make a tea use it for soaking grains and for hydration of substrate. You will never see green again. I got that tip from home mycology. It’s the tannins.
Got the video? I’d like to see it
@@Mycophilia I went looking for the video on the home mycology channel couldn’t find it. Perhaps the forums have some reference to it somewhere. I would say it doesn’t hurt.Im not scientific enough to do a extensive experiment but perhaps some one somewhere has . I think he just credited the hippie for the tec .
@@Mycophilia will you feature this in an experiment please?
Great video
Thanks!
Can you grow the good bacteria and then sterilize and then add the growing good bacteria?
Probably but that’s kinda beyond me to isolate the good bacteria.
@@Mycophilia with cannabis they make rooting hormones and good micrbos and have plenty from my growing days. have you tried any?
@@gangsterbuilder no but mushrooms are completely different organisms than plants
@@Mycophilia humans were a fungus a few 100 million years ago.
Few people know that we are more closely related to fungi than to any other kingdom 650 million years ago
@@gangsterbuilder yes I’m aware
What is the purpose of the casting layer?
Check out my casing layer video (type mycophilia casing layer in the YT search bar)
Love it
Thanks, glad to hear that!
If you can pasteurize nutritious substrate, could you theoretically pasteurize grain for spawn?
No as grain is far too nutritious. There’s a reason they’re sterilized.
Thanks for sharing... Keep up the great work!
Thank you! Will do!
Lebanon 🌲🌲🌲
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Lebanon
You talk about pasteurization time and temperatures, but you're not talking about how you're pasteurizing. Are you using an over? Are you boiling? or???
In jars inside a slow cooker
@@Mycophilia Correct me if I'm wrong, but I haven't seen you make a video about that. That would definitely be interesting as I haven't seen that tek b4
@@DangerRussDayZ6533 I use to have one but I’ll repost/remake it!
@@Mycophilia That would be great. I'm also interested in your coir only tek, because I believe I heard you mention that you do not need to pasteurize coir if you're only using coir. I'll definitely be staying tuned for this video, if you decide to make it. Thanks
@@DangerRussDayZ6533 👍
I'm sorry but I've done both and have zero contam with pasteurization. I won't sterilze anymore.
Why are you sorry lol
How do you pasteurize your substrate?
12 hours on the keep warm setting in an instantpot
Substrate brought to field capacity, and contained in jars
Saga: I never had good luck with the Moo Cow Poo that has wood in the mix, this is like opening the door for the Mean Green.
That is interesting that you mention the wood, as I had my suspicions about that as well. Thanks for the comment Dave!
What if you did small isolated sterilizations instead of pasteurizations, with an overnight presoak in a Colloidal Silver solution? Are you scavenging for feces locally? If you're scavenging for local crap, and said local crap has wood, the wood has been through the digestive tract and there's minimal you can do to get the contaminants out of that wood especially if it's bovine. The digestive system basically softens the wood and it will absorb everything inside the cows body it comes into contact with during the digestive process. In that case I would still recommend small isolated batches however during the pasteurization/sterilization (whatever you choose) increase the length of your process by atleast 3x if you can get the wood poop to maintain a temp around 170°F for long enough it should have an effect but still add Colloidal Silver with your field capacity process as that will either eliminate or retard the process so much you'll get a fruiting and a flush or 2 I would hope. I've been experimenting with Colloidal Silver a bit with great success. And the mycelium doesn't mind it much at all. The tomentose growth may retreat a bit, however it will come right back with a fervor. It may turn your mycelium blueish, I'm not sure if my blues were from the silver or the "Silo Sin" oxidizing within the mycelium, I know for a fact the blue was NOT contam. I've discovered that the mycelium will bruise blue when you touch it or cut specimens just like it's fruit, especially the higher percentage genetics. Apparently the mycelium has quite a bit of "blue love" lol. I plan to experiment With this by growing and harvesting a large sample of healthy mycelium and doing an extraction and distillation of the "contents" to see. I got the idea from some lab analysies of percentages of Cordycepin contained within the Cordyceps fruiting bodies as opposed to the mycelium. Cordycep Mycelium contains a noticably higher percentage of cordycepin than it's fruits.
@@treehouseconstituents6402 I’am really coming to suspect the poo now. Next time I’ll just buy a bag of pure cow poop and see how that fares, nonetheless I’m happy to see both of you talk about the wood as I had my suspicions. What tripped me up though was how some people don’t have a problem even with the wood. But all I know is that the casing layer is pasteurized fine with the same method every single time, whereas this compost contaminated 100% of the time. I’m going to try pure cow manure
@@Mycophilia lmk how that works bro, I'm still doing synthetic dung with pharmaceutical grade chemical nutrients, I make a nutrient broth out of Pure nitrogen crystals, humic acid extract from coal and compost, phosphorus, kelp extract, sodium, Calcium, magnesium, Sulphur, cellulose, flavonoids and polyphenols. It's a little harder to locate pure polyphenols and flavonoids at a reasonable price though.
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remember pasteurization and sterilization is useless if you have contaminated spawn even if you dont see it
Not necessarily, I’ve seen a bit of contam get beaten by mycelium before, both in tubs and on agar plates. That’s what I’m always telling these growers who show a small spot of contam on their second flush, and calling it ruined as you’ve got another ounce of dried weight that you can harvest tomorrow, it’s not a death sentence don’t throw it out.
I always just run with it and give the mycelium the best opportunity to outgrow it. These things grow in the wild.
SUPER PASTEURIZATION
Horse manure works the best i get dried poo balls from a friend with a horse
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