Just a minute or two in and I am already a bit excited to see that burner. I have been doing some mycology lately and all of the videos in that space show use of flow hoods and still air boxes; but I picked up sterile technique using a bunsen burner and found it so much easier. Great to see you preparing to demonstrate it. So much easier than trying to setup a chamber just to have a sterile field. Edit: Now that I have watched a good chunk, I would love to see you do some examples of fungal microscopy, particularly slide cultures. I really want to try the technique where you grow a culture under a cover slip then mount that slip upside down under a larger one.
Hello MicrobeHunter, the livestream was amazing !! But I have a few questions: 1. What if I don’t have an autoclave? 2. What if I don’t have an inoculation tube??
Thank you for the response!! But I also want to know how to make those agar agar plates but instead of E. coli pills, I use yogurt instead? And also, what kind of yogurt? My family makes yogurt at home. Anyway, thank you for responding!!!
Watch this video here, it explains how to observe yogurt bacteria: th-cam.com/video/PdDAgwTB4-0/w-d-xo.html This is much better than making agar plates (it is safter).
Thank you for the response. Can I ask specifically how to actually make the agar agar plates??? Step by step, I mean. And also, can I actually put yogurt directly on the agar agar plates? What mounting medium can I use to make a permanent slide of these stained bacteria? And finally, how to make the inoculation loop? Sorry for asking so many questions.
its really appericiable and kindly handle microbes with care and wear gloves while handling ( better to perform in Laminar air flow) and one more doubt agar media doesnt pluged with cotton plug only covered with aluminium foil and why?
I actually disagree that flow hoods are better. They are better if you are doing a lot of work, but for one-offs and home hobbyists, burners are very simple and very effective. I regularly pour and inoculate small stacks plates under a flame and have no complaints at all about contamination rates
Very great instructional video.
You point out the common error and reason you do thing the way you do it.
Thank you for sharing your knowledge.
Love it as always !
Good explanation.
Just a minute or two in and I am already a bit excited to see that burner. I have been doing some mycology lately and all of the videos in that space show use of flow hoods and still air boxes; but I picked up sterile technique using a bunsen burner and found it so much easier. Great to see you preparing to demonstrate it. So much easier than trying to setup a chamber just to have a sterile field. Edit: Now that I have watched a good chunk, I would love to see you do some examples of fungal microscopy, particularly slide cultures. I really want to try the technique where you grow a culture under a cover slip then mount that slip upside down under a larger one.
Hello MicrobeHunter, the livestream was amazing !! But I have a few questions: 1. What if I don’t have an autoclave? 2. What if I don’t have an inoculation tube??
Autoclave = pressure cooker (for food). Inoculation loop: can be bought over amazon or DIY.
Thank you for the response!! But I also want to know how to make those agar agar plates but instead of E. coli pills, I use yogurt instead? And also, what kind of yogurt? My family makes yogurt at home. Anyway, thank you for responding!!!
Watch this video here, it explains how to observe yogurt bacteria: th-cam.com/video/PdDAgwTB4-0/w-d-xo.html This is much better than making agar plates (it is safter).
Thank you for the response. Can I ask specifically how to actually make the agar agar plates??? Step by step, I mean. And also, can I actually put yogurt directly on the agar agar plates?
What mounting medium can I use to make a permanent slide of these stained bacteria?
And finally, how to make the inoculation loop? Sorry for asking so many questions.
Where can I find a burner like that one? 🤔
Are there any petri dishes that have tight fitting lid? I would like to have pertri dishes with tight fitting lid but not able to find any...
its really appericiable and kindly handle microbes with care and wear gloves while handling ( better to perform in Laminar air flow) and one more doubt agar media doesnt pluged with cotton plug only covered with aluminium foil and why?
I actually disagree that flow hoods are better. They are better if you are doing a lot of work, but for one-offs and home hobbyists, burners are very simple and very effective. I regularly pour and inoculate small stacks plates under a flame and have no complaints at all about contamination rates
A cheaper option to grow known safe bacteria:
just take some from your plain yogourt.
Between 25:00 and 35:00 the video stops and is unwatchable. The stops and starts