Looking forward to reading through your book! This is the most informative and direct mycological channel on the tube, people. Tuned-in with the bell here!
once inoculating the petri dish how long will it take a lion's mane to show up. And how do I set the humidity on the petri dish for the mycelium to grow?
There's away to, when you're recording your videos to make them turn out really loud after recording. You either turn your volume down low or turn it up high to make your videos have much greater sound playback! A viewer can always turn the volume down if it's to loud, but can only turn it up so high when they simply cannot hear it, like this video here! You put in all that time and work making a video that the viewers can't even hear, that's frustrating mostly for the viewers! I hope you understand why I wrote this. It's just to bring to your attention that I do like your work, but I like so many millions of others can't hear that well and are trying to figure out how we can in some small way help. Hopefully you're next video will be so loud, I will actually have to turn it down, lol. Thanks! th
Thank you for your feedback! These were pretty bad.... recorded portrait on an iphone. Ha! We've upgraded our equipment significantly and this won't be a problem anymore. Thanks for bearing with us 🙏
Thanks for the great video. I followed the link to the website but don;t see anywhere to download the ebook. There are other ebooks but I can't see this one.
When testing liquid culture on agar, how many days should you give the agar plate to colonize before assuming your liquid culture clean and ready to inoculate jars??
Good work! Some buildings are more problematic than others so it all depends on your unique conditions. We had a lot of $ on the line and contamination was not an option so we take precautions! Mush love!
I suppose so, if you can make it work, but agar will give you a more accurate panel of the organisms. For example, trich would be easy to identify on grain, but bacterial contamination would not be. Agar gives you a much clearer idea. Hope that helps! May the spores be with you!
How do you destroy the trichoderma petri dishes, isn't that special waste that has to be disposed of in a special way so that the environment is not polluted? Or is it simply burned in the waste facilities ? Thank you :)
After you squirt 1 cc of your liquid culture on to your agar plate, do you put it in an incubator? If so, what temp and for how long before you get some reliable growth that you can differentiate?
We just store it with the other liquid culture jars, so in a cool dark room, about 67-69 degrees. You can see growth as soon as the next day, but 3-5 days will express a true panel of the lc.
I wish I could see what good mycelium looks like. All videos that I’ve seen so far either don’t show a close up or a close up that is so out of focus it does no good
They are definitely still viable and up to you if you want to use them. The slower growing ones can also be because you deposited less mycelium on the agar, just try and be consistent and observant to make that call! if they are growing a lot less slow than the rest, we tended to just dump them. Usually however, as long as they were not contaminated, we used them and they worked great.
So, I assume the liquid culture contains some kind of grain-based sugar extract along with spores that have germinated that have colonized into mycelium and the agar in the dish also has some kind of malt extract nutrient? Just want to be sure because I plan on moving towards LC and I don’t want too much sugar with toxicity being an issue of course.
The LC recipe here is just RO water and honey! We did start adding a teaspoon of malt to quicken growth, however. We also did not work with spore, but rather a single colony transfer from agar to liquid. If you are using cordyceps, a table spoon of honey per quart is a great recipe. Cordyceps are sort of sugar addicts and seemed to thrive the more nutrients we added. That said, increase little by little and monitor haha
@@MushroomRevival Hello Sir , do you have a video link/source how to make LC recipe for a beginner like me? Is it just a raw honey and sterilized water only is enough? What is the composition? Is MEA can be add to LC Recipe for boosting the progress? Thank you very much. From Indonesia 🙏🏻
Hi, do you need the flow hood and have to wear all of the sterile gear when doing the testing? Or was that for when cultivating the liquid culture? Also, is there a need to test liquid culture you bought online from a reputable source? Thank you, love what you do!!
a flow hood is very helpful but you can get away without one if your space is clean and you are still. The gear is not necessary for this test. If bought from a reputable source I would not worry too much, but still might be smart to squirt some on agar as back up and just to be safe!
Thank you for the great video. I was wondering if the agar test plates are also viable to innoculate as well? I cant see why not but i dont see anyone doing liquid to agar... also i am having a hard time locating the e book... could you set me up with a link?
Yes you can totally use the lc tests as a normal petri plate and expand as usual. Just a warning with cordyceps, they will senesce quicker with transfers, but most other basidiomycetes should be just fine. As for the e-book we mentioned, you can find it here: www.mushroom-revival.com/mycoremediation-handbook-e-book/ Bless!
We bought a premix, mostly to save time. Fungi Perfecti has good medium, as does seaweed solutions! If you want to make your own from scratch I suggest skimming TH-cam a bit more, theres lots on here.
good informational video but i spotted something you advise to use just plain malt extract agar to test your liquid cultures yet on the petri's you used to show in the video it says MEA (the antibiotic agar) so thats a funny little mistake there
Nourysh nope, a sister organic farm does that for us. It’s pretty easy to do. They grow in supplemented sawdust bags and if you cut slits in the sides they will grow out.
When Matz said the growth was slower/light on a couple plates....wouldn't that just mean not enough mycelium growth yet?? Wait another week and test again.
To sterilise you can preheat the oven on 300°F and put your jars in there once its sone pre-heating, and once it starts bubbling its done, it takes about 50 minutes
The vectors are many. Dirty cultures, extremophile surviving the cook, imperfect technique.... its rare for us, but happens occasionally with wild clones.
This woman did seem soo sympathyc, kind, shy and inteligent :) its un-common to have mycologist woman's ! Keep on propagating ! You huys have an enormour amount of L.C. hoho
My mate had a setup like this. I put a sandwich inside his laminar flow box and he never found it but quit 6 months later because everything he had got horribly contaminated and he could no longer fulfill orders.
what a beauty!
2:13 is where the actual video starts. Thanks Mat!
Lol, mushroom growers are the worst at staying on topic, idk why.
I love that you plant a tree for every sold product. Great job, wish you all the best!
We sure do! We've planted over 40,000 so far! Let's save the planet! Mush love. 🍄❤️
Looking forward to reading through your book! This is the most informative and direct mycological channel on the tube, people. Tuned-in with the bell here!
The work you guys do sharing this with us is invaluable.... Thank you so much!!
So glad we could help!
You guys are great.Keep those videos coming MushLove.
Beautifully explained, thank you
I'm making my first jars and this is exactly what I was needing. Thak you, very clear.
Thanks for tuning in, glad we could help!
You guys are amazing!!! Mush love
once inoculating the petri dish how long will it take a lion's mane to show up. And how do I set the humidity on the petri dish for the mycelium to grow?
Goals, great info from the team! Thank you so much!
So glad it was helpful!
wheres this e-book? I follow the link, and it takes me to your home page, where I cannot find this e-book..
4:26 which antibiotics do you use and can I get them from the medical store?
There's away to, when you're recording your videos to make them turn out really loud after recording. You either turn your volume down low or turn it up high to make your videos have much greater sound playback! A viewer can always turn the volume down if it's to loud, but can only turn it up so high when they simply cannot hear it, like this video here! You put in all that time and work making a video that the viewers can't even hear, that's frustrating mostly for the viewers! I hope you understand why I wrote this.
It's just to bring to your attention that I do like your work, but I like so many millions of others can't hear that well and are trying to figure out how we can in some small way help. Hopefully you're next video will be so loud, I will actually have to turn it down, lol. Thanks!
th
Thank you for your feedback! These were pretty bad.... recorded portrait on an iphone. Ha! We've upgraded our equipment significantly and this won't be a problem anymore. Thanks for bearing with us 🙏
Thanks for the great video. I followed the link to the website but don;t see anywhere to download the ebook. There are other ebooks but I can't see this one.
when you squirt the LC on the agar do you roll it around or just let it sit in a droplet?
Great question! Rolling it around a bit is a good idea, but either way it'll do the trick!
Where do you store the agar in light or dark space and is it at room temperature?
When testing liquid culture on agar, how many days should you give the agar plate to colonize before assuming your liquid culture clean and ready to inoculate jars??
where do you get those metal trays to work on in front of the flow hood?
What size of needle do you use in the transfer. I worry that too small a bore might not take up the mycelium.
18g is good!! 🙌
You guys are awesome! Thank you so much for sharing your knowledge with us! Peace and love ✌🏻❤️
peace and love to you too, happy to help 🙏
Do you heat the needle to red hot before you insert into you LC Jar?
Not if its a new needle, but if its used, yes : )
Mushroom Revival thanks
Hello guys do you still have the book available? THANKS!
Absolutely! Here you go: drive.google.com/file/d/1BTu5__UXdBqti4RspvHy8nd6gJaEY2hH/view?usp=sharing 🤗🍄
I make agar in ketchup cups in a kitchen no flow hood open air works perfectly
Hell yeah, that's awesome! Never been that lucky ourselves 😔
@@MushroomRevival small room no air flow and some Lysol and f2f🍄💗
Good work! Some buildings are more problematic than others so it all depends on your unique conditions. We had a lot of $ on the line and contamination was not an option so we take precautions! Mush love!
@@MushroomRevival I hear you if I were doing it on a commercial grade I would definitely invest in one myself
hey question do you sterilize the ketchup cups? i have a pack and dont know what to do. dunk them in alcohol?
What was the intro song? It was a banger!!!
So how long does the test take?
Thanks for sharing what you have learned! This instruction was very easy to follow.
So glad you liked it!
How many drops and how long, on average before you have visible growth?
definitely a great tip ill keep it in mind thanks
happy to help 🙏
Is your book in PDF I don't have an Amazon Kindle account
Yes it is! Please email support@mushroomrevival.com and they can hook you up with the link! 🍄👍🙃
where do you guys buy the injection ports ? thank you :)
you can make it out of RTV silicone from any autoparts or hardware store
Amazon 10$ for 100
Literaly a great way to make an injection port ! And u can transform any glass container or botle into something usefull , when u have r.t.v.
how to video on sterilizing and storing used needles is it wise? I refer to the book all the time
A flame, baci-cinerator, or alcohol will do the trick for sterilizing the tips :)
Is the Lumen on a i sulin syringe big enough to allow mycelium to pass through it?
You bet!
Thank you for the information your sharing.
Happy to help!
Can i use a very small grain spawn instead of the agar, like 2 grain thick??
I suppose so, if you can make it work, but agar will give you a more accurate panel of the organisms. For example, trich would be easy to identify on grain, but bacterial contamination would not be. Agar gives you a much clearer idea.
Hope that helps! May the spores be with you!
How do you destroy the trichoderma petri dishes, isn't that special waste that has to be disposed of in a special way so that the environment is not polluted? Or is it simply burned in the waste facilities ? Thank you :)
Nope - trich is everywhere and actually a wonderfully beneficial soil fungi! Throwing in the compost is good :
@@MushroomRevival Ah, thank you :)
I need book please
Great video BTW
After you squirt 1 cc of your liquid culture on to your agar plate, do you put it in an incubator? If so, what temp and for how long before you get some reliable growth that you can differentiate?
We just store it with the other liquid culture jars, so in a cool dark room, about 67-69 degrees. You can see growth as soon as the next day, but 3-5 days will express a true panel of the lc.
I wish I could see what good mycelium looks like. All videos that I’ve seen so far either don’t show a close up or a close up that is so out of focus it does no good
So are the ones that are not infected but are "not as happy" just no good and need pitched or just need more time? What's going on with those?
They are definitely still viable and up to you if you want to use them. The slower growing ones can also be because you deposited less mycelium on the agar, just try and be consistent and observant to make that call! if they are growing a lot less slow than the rest, we tended to just dump them. Usually however, as long as they were not contaminated, we used them and they worked great.
Could you further isolate the contaminated plates? Like save face?
Yes, you could if you have to! One tip is to keep the contam BEHIND the clean portion in relation to the flowhood
@@MushroomRevival thanks a billion yo
Of course! MUSH LOVE! 🍄
@@MushroomRevival blessed are the mycelious. Fung love fam
🙌 🍄 💛
hey what size petri dishes are those ? Also love everything yall do great pod casts !!!
sleepyalligator86 100 60mm
So, I assume the liquid culture contains some kind of grain-based sugar extract along with spores that have germinated that have colonized into mycelium and the agar in the dish also has some kind of malt extract nutrient?
Just want to be sure because I plan on moving towards LC and I don’t want too much sugar with toxicity being an issue of course.
The LC recipe here is just RO water and honey! We did start adding a teaspoon of malt to quicken growth, however.
We also did not work with spore, but rather a single colony transfer from agar to liquid. If you are using cordyceps, a table spoon of honey per quart is a great recipe. Cordyceps are sort of sugar addicts and seemed to thrive the more nutrients we added. That said, increase little by little and monitor haha
@@MushroomRevival Hello Sir , do you have a video link/source how to make LC recipe for a beginner like me? Is it just a raw honey and sterilized water only is enough? What is the composition? Is MEA can be add to LC Recipe for boosting the progress? Thank you very much.
From Indonesia 🙏🏻
wow nice flow hood(s)...
Thanks!! May the spores be with you! 🍄
Hi, do you need the flow hood and have to wear all of the sterile gear when doing the testing? Or was that for when cultivating the liquid culture? Also, is there a need to test liquid culture you bought online from a reputable source? Thank you, love what you do!!
a flow hood is very helpful but you can get away without one if your space is clean and you are still. The gear is not necessary for this test. If bought from a reputable source I would not worry too much, but still might be smart to squirt some on agar as back up and just to be safe!
Thank you for the great video. I was wondering if the agar test plates are also viable to innoculate as well? I cant see why not but i dont see anyone doing liquid to agar... also i am having a hard time locating the e book... could you set me up with a link?
Yes you can totally use the lc tests as a normal petri plate and expand as usual. Just a warning with cordyceps, they will senesce quicker with transfers, but most other basidiomycetes should be just fine. As for the e-book we mentioned, you can find it here: www.mushroom-revival.com/mycoremediation-handbook-e-book/
Bless!
@@MushroomRevival thank you!
Wow that ebook is awesome, straight forward instructions.
Great video. Thanks. I assume agar with 5% Karo syrup will work fine as well for petri dishes?
We have no used Karo, but I see many recipes with Karo so I believe it will work well!
What is a good recipe for making agar?
We bought a premix, mostly to save time. Fungi Perfecti has good medium, as does seaweed solutions! If you want to make your own from scratch I suggest skimming TH-cam a bit more, theres lots on here.
good informational video but i spotted something
you advise to use just plain malt extract agar to test your liquid cultures yet on the petri's you used to show in the video it says MEA (the antibiotic agar)
so thats a funny little mistake there
Nope, MEA = malt extract agar
we mark antibiotic as MEA+
great video!!
your so beautiful and extremely knowledgeable wow 👏
Do you guys cultivate turkey tail? Would love to see a guide on that if you do.
Nourysh nope, a sister organic farm does that for us. It’s pretty easy to do. They grow in supplemented sawdust bags and if you cut slits in the sides they will grow out.
@@MushroomRevival what kind of supplement do they add to saw dust. Thanks
When Matz said the growth was slower/light on a couple plates....wouldn't that just mean not enough mycelium growth yet??
Wait another week and test again.
Yes, very possibly there was not enough mycelium. We have just noticed that this is also an indicator of less robost cultures. :)
How do you if ur liquid culture is contaminated I made a honey one pc for 30 minutes did I do too much
To sterilise you can preheat the oven on 300°F and put your jars in there once its sone pre-heating, and once it starts bubbling its done, it takes about 50 minutes
i do 90 minuts for liquid culture
Thx guys (´n gurls), helped me a lot !!
You are so welcome!! Thanks for shrooming in! 🍄💯
whats the purpose of the red light
Probably growing cannabis in the background.
For goof vibes and less harsh lighting on cultures :)
These people are so full on hippies, you know good and well they know what they're doing when it comes to growing good mushrooms.
Agar with antibiotics?
We have a video on that! Check out our liquid culture video.
I am from India
I wonder how it got infected in the first place.
The vectors are many. Dirty cultures, extremophile surviving the cook, imperfect technique.... its rare for us, but happens occasionally with wild clones.
Jimi Hendrix!!!!
does anyone els clean cultures with H2O2
Yes, we've used h2o2 in our lab, but we prefer isopropyl 🙏
This woman did seem soo sympathyc, kind, shy and inteligent :) its un-common to have mycologist woman's ! Keep on propagating ! You huys have an enormour amount of L.C. hoho
She is all of those things and more!
My mate had a setup like this. I put a sandwich inside his laminar flow box and he never found it but quit 6 months later because everything he had got horribly contaminated and he could no longer fulfill orders.
What a *DICK MOVE*
Next level pranking.... don't come near our farm with a sandwich!
you are kidding right? damn you're playing with his livelihood
Is she single?
taken ;)
What about now? 😉
She's very beautiful she makes the videos.ok what was she talking about sorry
2 mins of BS. Test the culture on agar. Video done. Simple and no wonder the girl looks bored.
We just like to be detailed since many people are super new to the tech! :)