MALDI TOF Mass Spectrometry | Deconvolution of Mass Spectra | Dr. Nagendra Singh | PENS#74

#MALDITOF #massspectroscopy #MALDI
MALDI TOF Mass Spectrometry | Deconvolution of Mass Spectra | Dr. Nagendra Singh | PENS#74
In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation. It has been applied to the analysis of biomolecules (biopolymers such as DNA, proteins, peptides and carbohydrates) and various organic molecules (such as polymers, dendrimers and other macromolecules), which tend to be fragile and fragment when ionized by more conventional ionization methods. It is similar in character to electrospray ionization (ESI) in that both techniques are relatively soft (low fragmentation) ways of obtaining ions of large molecules in the gas phase, though MALDI typically produces far fewer multi-charged ions.
MALDI methodology is a three-step process. First, the sample is mixed with a suitable matrix material and applied to a metal plate. Second, a pulsed laser irradiates the sample, triggering ablation and desorption of the sample and matrix material. Finally, the analyte molecules are ionized by being protonated or deprotonated in the hot plume of ablated gases, and then they can be accelerated into whichever mass spectrometer is used to analyse them
The Lectures are given by Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) New Delhi. Dr. Singh has a vast experience of research and Teaching in the fields of Structure & Molecular Biology and Biochemistry, which also include Biomolecules, Proteomics, Protein Engineering and Drug Design.
Dr. Nagendra Singh currently works at the School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA (www.gbu.ac.in/FacultyProfiles/.... He does research in Molecular Biology, Structural Biology and Biotechnology. His current research interest include 'Structural and functional characterization of proteins involved in RNA metabolism from human and microbial origin. Dr Singh has published over 70 research articles in intentional Journals of good repute. He has over 20 awards and honors on his name. Few of the awards include "National Academy of Sciences of India (NASI, Allahabad) young scientist award", UGC-NET, CSIR-JRF, GATE, Best Research Awards etc. Dr. Singh has participated and presented research work at over 50 national and International conferences world wide.
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[@shree_12]

Thank you so much sir 🙂

[@chrisjzahner]

This lecture was wonderful. Thanks so much!

[@proteinengineering007]

I am glad you find it useful @Chris Zahner

[@mohanchaitanyareddy7947]

Thank you. the lecture was great.

[@proteinengineering007]

i m glad you like it @MOHANCHAITANYA REDDY