As a layperson with regards to virology I nevertheless find these and associated podcasts like TWIV etc exceptional and reassuring, that there are such knowledgeable and experienced people in such subjects. Fantastic effort everyone, especially Vincent. 👌👍
As a working pharmaceutical microbiologist, I could listen to your podcasts forever and get no work done! Thank you so much for putting these out and letting us into your world and minds. These have become priceless to me and a part of my daily routine. I re-listen just to catch all the things I didn't hear the first time around!
You guys are very cool. Takeaways, 1) "Plaque essays are the engine of virology". 2) Rough Drafts should be strictly off the record for media and 3) tracking clinical signs to specific strains is a brilliant idea.
I'm a clinician & I admit I couldn't care less about origin being pangolin vs civet cat vs lab (I'll leave that to the geneticists & politicians). However, we care very much about what appear to be clinical differences in the strains we're seeing where I practice in NYC (thought to be the Europe/ NY strain) vs California (thought to be the Asian strain). We've had visiting physicians here coming from the West Coast & they all say that what they're seeing in NY doesn't look like what they've seen back home. Granted, we're dealing with much higher volume of cases here & these are anecdotal accounts of the differences, but they're consistent across all the visitors. Cali's data seems questionable to me, too, bc I simply don't believe they'd have over 40k cases of NYC covid without more folks on ventilators. Can you review literature on clinical differences of the strains?
I think that the first steep in pathologic chain isn overstimulation of immune response by viremia The second is increased IgM level and haemagglutination If temperature of plasma are low Haemagglutination can be reversibile by heating plasma or under Dextran40 Haemagglutination in periferial blood can obstruct capillaires and can be reflected making a blood samples in a glass for mycroscope and seen Roll of eritrocyte like in macroglobulinemia Waldenstorm
Saeed AlAmoudi this virus is thought to be traveling by the water droplets in the air. So hot humid air means there is more water in the air but very heavy ! This heavy water droplets is thought to fall to the grown faster which will lead to a lot less transmissions. However, just like the common cold is still being transmitted in the summer so can this but will be highly reduced. Uv rays can absolutely destroy a strand of RNA and protein , just think about what the sun can do to you !!!
In hypothesis, it can reduce the rate of transmission. In reality, without physical distancing, and other protection methods is as deadly and contagious as in cold, less humidified and lower sun light places. Take for example the city I live in. Guayaquil- 88F/31C, 75% humidity, UV index 14 on average. We have triple and quadruple the covid19 related deaths in the last 2 months.
Wow, thank you so much for sharing this conversation. It's really fascinating and even inspiring me to learn. I'm auditing your 2020 virology course and really enjoying learning. I think this subject is itself viral.... It's recruiting the machinery of my brain (whatever remains).
Sir could you please say something about the low death rates in the Indian subcontinent? Testing is not as extensive as in the US. The countries are incredibly crowded, people are doing only a perfunctory social distancing. In Pakistan people are openly defying the lock down. In Bangladesh thousands of people crowded together for a funeral. But for all that death rates are surprisingly low. It would be very informative if you could do a broadcast on this interesting situation.
Hello, I will let the podcaster address this, too but I practice medicine in the US & there is some belief that (1) those countries have a younger population than Western countries, so you simply don't have as many of the vulnerable people who would pass away if they get the illness; & (2) there is strong sunlight in some of those areas that means the virus doesn't last as long on surfaces, & coupled with lack of underground metro subway like NYC, you have less surface transmission. But #1 is considered the main reason for low mortality rate.
Since I have no real scientific background (only a little basic pharmaceutical education) I can't truly talk about anything scientific regarding the origin of SARS-CoV-2, but I think I can tell what I would think about the possibility of the lab escape/release theory. In my humble opinion, it can't be ruled out completely. There might be good clue or even evidence meanwhile that the virus has been circulating in animal hosts, has been naturally recombined and so on. Still it could be possible that not all lab work got a protocol. It could be possible the work on samples from field studies went unchecked. That's something I actually can imagine since I'm convinced that humans will do whatever is physically possible (including e.g. making lab generated virus genome mutants that would not be any kind of intelligent approach to willful biological terrorism or what so ever - just because it's possible to make them). And there is maybe a good amount of distrust out there among the people towards China as a communist country and also as a rival to other global players on the international market, trying to assure its own advantage over other nations. I'd be actually surprised if every participant would be playing by the rules. That includes people breaking the laws, people lying and hiding things, people not caring about safety/security measurements and also people doing stupid lab experiments. (Anyway, I guess of course that isn't limited just to China but is for sure a worldwide phenomenon.)
One way to minimize it is use the Receptor Binding Domain of the Spike protein. That domain exhibits very low similarity to the other coronaviruses (except SARS1).
As a layperson with regards to virology I nevertheless find these and associated podcasts like TWIV etc exceptional and reassuring, that there are such knowledgeable and experienced people in such subjects. Fantastic effort everyone, especially Vincent. 👌👍
Incredible what one can learn from Prof. Racaniello about terminology in this field of science, always impressed.
As a working pharmaceutical microbiologist, I could listen to your podcasts forever and get no work done! Thank you so much for putting these out and letting us into your world and minds. These have become priceless to me and a part of my daily routine. I re-listen just to catch all the things I didn't hear the first time around!
You guys are very cool. Takeaways, 1) "Plaque essays are the engine of virology". 2) Rough Drafts should be strictly off the record for media and 3) tracking clinical signs to specific strains is a brilliant idea.
I'm a clinician & I admit I couldn't care less about origin being pangolin vs civet cat vs lab (I'll leave that to the geneticists & politicians). However, we care very much about what appear to be clinical differences in the strains we're seeing where I practice in NYC (thought to be the Europe/ NY strain) vs California (thought to be the Asian strain). We've had visiting physicians here coming from the West Coast & they all say that what they're seeing in NY doesn't look like what they've seen back home. Granted, we're dealing with much higher volume of cases here & these are anecdotal accounts of the differences, but they're consistent across all the visitors. Cali's data seems questionable to me, too, bc I simply don't believe they'd have over 40k cases of NYC covid without more folks on ventilators. Can you review literature on clinical differences of the strains?
Scarlet totally agree with your comments. As a CA resident quite honestly we are just waiting around and nothing happens.
I think that the first steep in pathologic chain isn overstimulation of immune response by viremia
The second is increased IgM level and haemagglutination If temperature of plasma are low
Haemagglutination can be reversibile by heating plasma or under Dextran40
Haemagglutination in periferial blood can obstruct capillaires and can be reflected making a blood samples in a glass for mycroscope and seen Roll of eritrocyte like in macroglobulinemia Waldenstorm
Very helpful. Many thanks. I'm glad to have this access to thoughtful, educated discussion.
What is the effect of increased temperature, humidity and sunlight on SARS-CoV 2?
Saeed AlAmoudi this virus is thought to be traveling by the water droplets in the air. So hot humid air means there is more water in the air but very heavy ! This heavy water droplets is thought to fall to the grown faster which will lead to a lot less transmissions. However, just like the common cold is still being transmitted in the summer so can this but will be highly reduced. Uv rays can absolutely destroy a strand of RNA and protein , just think about what the sun can do to you !!!
In hypothesis, it can reduce the rate of transmission.
In reality, without physical distancing, and other protection methods is as deadly and contagious as in cold, less humidified and lower sun light places.
Take for example the city I live in.
Guayaquil- 88F/31C, 75% humidity, UV index 14 on average.
We have triple and quadruple the covid19 related deaths in the last 2 months.
“Plaque assays prevent literature contamination.”
what does that mean??
I am glad that Australia gave them what they needed. It makes me proud to be Australian.
Yeah, it's worth bottling the bloke's blood that sent that. (or Sheila) Some of you cobbers are dinky-di!
Thanks a lot. Is that possible to include references of the paper you are talking about please? Best regards
www.medrxiv.org/content/10.1101/2020.04.14.20060160v2.full.pdf this one?
Page 40
Wow, thank you so much for sharing this conversation. It's really fascinating and even inspiring me to learn. I'm auditing your 2020 virology course and really enjoying learning. I think this subject is itself viral.... It's recruiting the machinery of my brain (whatever remains).
Sir could you please say something about the low death rates in the Indian subcontinent? Testing is not as extensive as in the US. The countries are incredibly crowded, people are doing only a perfunctory social distancing. In Pakistan people are openly defying the lock down. In Bangladesh thousands of people crowded together for a funeral. But for all that death rates are surprisingly low. It would be very informative if you could do a broadcast on this interesting situation.
Hello, I will let the podcaster address this, too but I practice medicine in the US & there is some belief that (1) those countries have a younger population than Western countries, so you simply don't have as many of the vulnerable people who would pass away if they get the illness; & (2) there is strong sunlight in some of those areas that means the virus doesn't last as long on surfaces, & coupled with lack of underground metro subway like NYC, you have less surface transmission. But #1 is considered the main reason for low mortality rate.
Have Covid19 some crosslinked linked antigen , epithops who can explain a prezumtive haemagglutination in presence of high level of IgM?
Since I have no real scientific background (only a little basic pharmaceutical education) I can't truly talk about anything scientific regarding the origin of SARS-CoV-2, but I think I can tell what I would think about the possibility of the lab escape/release theory.
In my humble opinion, it can't be ruled out completely. There might be good clue or even evidence meanwhile that the virus has been circulating in animal hosts, has been naturally recombined and so on.
Still it could be possible that not all lab work got a protocol. It could be possible the work on samples from field studies went unchecked. That's something I actually can imagine since I'm convinced that humans will do whatever is physically possible (including e.g. making lab generated virus genome mutants that would not be any kind of intelligent approach to willful biological terrorism or what so ever - just because it's possible to make them).
And there is maybe a good amount of distrust out there among the people towards China as a communist country and also as a rival to other global players on the international market, trying to assure its own advantage over other nations.
I'd be actually surprised if every participant would be playing by the rules.
That includes people breaking the laws, people lying and hiding things, people not caring about safety/security measurements and also people doing stupid lab experiments.
(Anyway, I guess of course that isn't limited just to China but is for sure a worldwide phenomenon.)
Vincent should be leading the task force.
How do they make sure serologic tests do not crossreact with common cold HCov ABs?
One way to minimize it is use the Receptor Binding Domain of the Spike protein. That domain exhibits very low similarity to the other coronaviruses (except SARS1).
We need more bat research, not less.
How many BSL3 labs can you hold in a bat cave? Millions? Billions?
More recombination research, not less
"50:30"
I couldn't imagine a tasty looking virus... LOL
Get your info straight ⚽️⚽️⚽️⚽️⚽️do ur homework for planDemic