I have made a home scale very small Bioreactor for growing up yeast cultures of Kveik Yeast for drying and freezing yeast slurry , Its a 1 quart to 1/2 gallon reactor depending on which jar i use. Basically its a ball airlock lid( the kind for fermenting vegetables with a built in airlock) that i drilled a second hole in and put a stainless steel air stone that is hooked to an air pump and it sits on a Stir plate, so, if my understanding of the process is correct this would be a Very Basic Bioreactor. My issue is the feeding schedule for optimum yeast growth and how much sugar and nutrient i should be feeding it daily. I have Fermax yeast Nutrient and I am doing my Grow up with white Table sugar because its a cheap easy sugar source. I am building up a personal yeast bank of Kveik yeast strains for use in Home wine,beer and mead making. So My goal is the highest biomass I can achieve with the limits of my home made set up. My reactor it self is working so far I am just not sure on a rough estimate of how much sugar and nutrient to feed it daily. I dont have a super accurate Gram scale(yet) so I am using measuring spoons and cups at this point At the present time, I am doing roughly 1/2 teaspoon of yeast nutrient and about 1/4 cup of sugar daily in a 1 quart volume of water I was wondering how these amounts look and if i should change them, if so how much of each would you suggest.
If i use plastic petri dishes will oven baking as per your video damage them? Also how long can i keep a petri dish culture for (assuming i tape it up around the sides) ?
They will melt. Plastic dished are single-use and sterile in the pack. Once cast, if you seal the edges with vinyl (electrical) tape they'll last a few months if stored in the refrigerator
I just purchased some agar, and I see now that it contains yeast extract and other nutrients. Will it be fine to use this one? Or do I have to buy clean agar with no additives? Thanks!
Sui Generis Brewing I just hope it won't interfere with what I have in mind. I will give it a shot. It's sucrose, yeast extract, and some other ingredients. Yeast will still survive, right?
Depends on what those other ingredients are. Some media contain things intended to suppress yeast growth - I don't suppose you have a link to the source, or could post the ingredients list. Without more detail I cannot say how it will work with yeast.
The ingredients are as following, in grams per liter (17,5g of powder per L of water): 5g Tryptone 2,5g yeast extract 1g dextrose 9g agar It is called 70152 plate count agar (casein-peptone dextrose yeast agar) The text in the instruction says "a non selective medium for the plate count of microorganisms in milk, other dairy products, food water and waste water. Thanks for your help!
That will work very well for yeast, it is very close to YPD media, just a little more nutrient-rich. YPD is used in scientific labs to grow yeast. Just add water and cast your plates/tubes!
question about streaking- are you lightly touching the transfer loop on the agar or are you scraping in to it? And are you use the face of the loop down or the edge of the loop down? I've been trying this and it looks if i put too much pressure, I gouge the agar and scrapes little channels. Or I may just not be using enough agar in my plates?
You want to be touching it lightly and not damaging the gel. You try to hold the loop at and angle of ~45 degrees relative to the gel, such that the part in contact with the gel is oppsite the direction you streak (e.g. you want to be dragging the part in contact with the gel; not pushing it). It sounds to me like you're either pusing too hard, or you're holding the loop flat/angeling it into your streak - both of those will tend to cut into the gel, which is not desired. If your gel is tearing, that's a sign you don't have enough agar; if you're simply cutting gouges into it, you're pushing too hard &/or angeling the loop incorrectly. It could also be an issue of the gel not having enough agar - generally you want 1.5% weight/volume agar - i.e. if making 100ml of gel, you'd add 1.5 grams of agar and 1-2g of DME (i.e. S.G of 1.004-1.006).
Hi, I purchased active dry yeast and the yeast/bacteria you are using is liquid so would I just put the dry yeast in regular water or does it have the a certain temperature or type?...sorry if this is a dumb question this is new to me.
If you've bought yeast you don't need to worry about plates - these are for storing yeast. Dry yeast should bee rehydrated in warm water for ~15 min and then added to your beer/wine/etc
Thank you for sharing your knowledge. Can you do a video on reproducing liquid yeast strains that is ready for brewing. In my country good liquid strains is hard to source, and ensure that they are in a healthy condition after being shipped.
What do you mean by "reproducing liquid yeast strains"? Do you mean running them through a starter in order to ensure they are healthy and increase their numbers?
Very good, thanks!
thanks lots for sharing your knowledge with us, home brewers, atleast it gives us a way to propogate our own yeast.....I hate buying yeast......
hehe
many thanks for sharing
I have made a home scale very small Bioreactor for growing up yeast cultures of Kveik Yeast for drying and freezing yeast slurry , Its a 1 quart to 1/2 gallon reactor depending on which jar i use.
Basically its a ball airlock lid( the kind for fermenting vegetables with a built in airlock) that i drilled a second hole in and put a stainless steel air stone that is hooked to an air pump and it sits on a Stir plate, so, if my understanding of the process is correct this would be a Very Basic Bioreactor.
My issue is the feeding schedule for optimum yeast growth and how much sugar and nutrient i should be feeding it daily. I have Fermax yeast Nutrient and I am doing my Grow up with white Table sugar because its a cheap easy sugar source.
I am building up a personal yeast bank of Kveik yeast strains for use in Home wine,beer and mead making. So My goal is the highest biomass I can achieve with the limits of my home made set up.
My reactor it self is working so far I am just not sure on a rough estimate of how much sugar and nutrient to feed it daily. I dont have a super accurate Gram scale(yet) so I am using measuring spoons and cups at this point
At the present time, I am doing roughly 1/2 teaspoon of yeast nutrient and about 1/4 cup of sugar daily in a 1 quart volume of water I was wondering how these amounts look and if i should change them, if so how much of each would you suggest.
Amazing stuff what a great resource! Thanks for subscribing as well!
Thanks for uploading these! I look forward to more!
Had all the gear for over 12 months , just gotta pull my finger out and give it a go.... Looking forward to it! Cheers!
Gash Slugg Good luck!
Great video do you have any where you view them under a microscope?
hail satan I have a video on choosing a microscope...probably pictures of yeast in it
Guess someone changed theyr username 🙈😄
When should u do the streaks? Should you do all of them at once or do a streak once a colony grows?
All at once. The idea is that each streak dilutes out the previous streak.
Thanks for sharing! 👍
More then useful! Also entertaining. Thank you.
Thanks!
If i use plastic petri dishes will oven baking as per your video damage them? Also how long can i keep a petri dish culture for (assuming i tape it up around the sides) ?
They will melt. Plastic dished are single-use and sterile in the pack. Once cast, if you seal the edges with vinyl (electrical) tape they'll last a few months if stored in the refrigerator
Thanks, awesome videos. Have been watching them almost all day. :-) Where can I source these Petry dishes with agar?
You have to make them. Plates & agar are available on ebay/amazon or via any scientific supply store.
I just purchased some agar, and I see now that it contains yeast extract and other nutrients. Will it be fine to use this one? Or do I have to buy clean agar with no additives? Thanks!
What kind of agar is it? Sounds like it is a prepared media; depending on what it is, it may or may not work
Sui Generis Brewing I just hope it won't interfere with what I have in mind. I will give it a shot. It's sucrose, yeast extract, and some other ingredients. Yeast will still survive, right?
Depends on what those other ingredients are. Some media contain things intended to suppress yeast growth - I don't suppose you have a link to the source, or could post the ingredients list. Without more detail I cannot say how it will work with yeast.
The ingredients are as following, in grams per liter (17,5g of powder per L of water):
5g Tryptone
2,5g yeast extract
1g dextrose
9g agar
It is called 70152 plate count agar (casein-peptone dextrose yeast agar)
The text in the instruction says "a non selective medium for the plate count of microorganisms in milk, other dairy products, food water and waste water.
Thanks for your help!
That will work very well for yeast, it is very close to YPD media, just a little more nutrient-rich. YPD is used in scientific labs to grow yeast. Just add water and cast your plates/tubes!
question about streaking- are you lightly touching the transfer loop on the agar or are you scraping in to it? And are you use the face of the loop down or the edge of the loop down? I've been trying this and it looks if i put too much pressure, I gouge the agar and scrapes little channels. Or I may just not be using enough agar in my plates?
You want to be touching it lightly and not damaging the gel. You try to hold the loop at and angle of ~45 degrees relative to the gel, such that the part in contact with the gel is oppsite the direction you streak (e.g. you want to be dragging the part in contact with the gel; not pushing it). It sounds to me like you're either pusing too hard, or you're holding the loop flat/angeling it into your streak - both of those will tend to cut into the gel, which is not desired.
If your gel is tearing, that's a sign you don't have enough agar; if you're simply cutting gouges into it, you're pushing too hard &/or angeling the loop incorrectly. It could also be an issue of the gel not having enough agar - generally you want 1.5% weight/volume agar - i.e. if making 100ml of gel, you'd add 1.5 grams of agar and 1-2g of DME (i.e. S.G of 1.004-1.006).
Sui Generis Brewing thanks!
Hi, I purchased active dry yeast and the yeast/bacteria you are using is liquid so would I just put the dry yeast in regular water or does it have the a certain temperature or type?...sorry if this is a dumb question this is new to me.
If you've bought yeast you don't need to worry about plates - these are for storing yeast. Dry yeast should bee rehydrated in warm water for ~15 min and then added to your beer/wine/etc
Oh ok, thank you
Thank you for sharing your knowledge. Can you do a video on reproducing liquid yeast strains that is ready for brewing. In my country good liquid strains is hard to source, and ensure that they are in a healthy condition after being shipped.
What do you mean by "reproducing liquid yeast strains"? Do you mean running them through a starter in order to ensure they are healthy and increase their numbers?
You did not perform it well the inoculating loop was out of flamed area many times and it causes contamination as well
And yet, there is no contamination on the plate. It's almost as though I've been doing this for decades and know how to work aseptically.
way cool
Recover yeast from an infected batch of beer? More please.