I have IDEXX ELISA kit for paratuberculosis. Formula is given there on brochure. OD values have to be incorporated in formula. But end results are confusing. I need help please.
In a perfect world, the blank well would read 0.0000, however this never happens, depending on the material of the plate, the temperature of the reader, the type of reader, each well will give off an absorbance value. This suggests that the blank well has some of your target analyte in it. However if you have completed the experiment correctly, your blank well does not have any analyte in it. So we must remove the value of the blank from each of the wells, to correct for atmospherics...
+abdulwahab KHASHAB Yes, if you would like to use the blank measurements on the standard curve that you use to interpret your data, then you should most certainly subtract the blank average from the blank values themselves.
I think you made a mistake calculating the background. You need to type in =average (b9:m9) but yours is =average (b9,m9) so you actually only selected two cells which made the background average very low.
You are amazing teacher. Thanks
Hi, thank you for the video It was very useful. One question, why are you dividing 500/3??? Thank you for the information
Very comprehensive. Thanks to your video. I was quite stuck with my assignment!
I have IDEXX ELISA kit for paratuberculosis. Formula is given there on brochure. OD values have to be incorporated in formula. But end results are confusing. I need help please.
very wonderful and simplified tutorial. I loved it
What is gray zone calculations that plus - minus 10% of CO value which is mentioned in literature of Elisa
Thanks a lot for your help, is so importan to my thesis
Hi is this applicable to direct ELISA too?
Thank you so much Very helpful. What was the BLANK? was it your negative?
thank you for very helpful tutorial , i want to ask how can calculate Absorbance data from Concentration value ?? Back up Equation
Great job
ery wonder and simplified tutorial. I loved it
nanananah esto es un plagio barato que gracias ni que leches
it is very usefull snd simplfied. thanks a lot. but then i have a query. why do you have to subtract the blank value from all the sample ODs?
In a perfect world, the blank well would read 0.0000, however this never happens, depending on the material of the plate, the temperature of the reader, the type of reader, each well will give off an absorbance value. This suggests that the blank well has some of your target analyte in it. However if you have completed the experiment correctly, your blank well does not have any analyte in it. So we must remove the value of the blank from each of the wells, to correct for atmospherics...
Thanks for your video✌✔🌹
vielen Dank. It´s realy very helpfull and easy to understand
Thanks McLovin
thank u so much , i m sorry i dont understood the part of concentration about 500 what is this number and how u r get it
+esmat shaker Nanodrop is one method to obtain concentrations. There are others
Very informative indeed, but i wonder why did you subtract your blank using your average blank?????
Blank measurements have some variation to them, so subtracting an average is a more accurate way to account for the assay's background signal.
Even with the blank itself!!!!! this is new to me.
+abdulwahab KHASHAB Yes, if you would like to use the blank measurements on the standard curve that you use to interpret your data, then you should most certainly subtract the blank average from the blank values themselves.
I'll try it out and compare both curves. Thanks allot
Why do you not make the -ve values into 0 since it can't have less than 0 antibody?
Thank you!
thanks a lot! this saved me
.TNF-alpha= 450 value - 0.132
Concentration - 11.792
Inme se result konsa hai.. please tell me
thx
I think you made a mistake calculating the background. You need to type in =average (b9:m9) but yours is =average (b9,m9) so you actually only selected two cells which made the background average very low.
Why are there -ve values?
nice tutorial but your voice really hard to put up with...