Here in this step we perform colony Hybridizarion technique used for screening of genomic library. In this step the bacterial colony is transfered to nylon membrane and denatured to expose the naked DNA . Then this membrane is soaked in a solution containing the labelled probe. Then washing is performed to remove nonspecific binding. The nylon sheet is sandwitched on Xray film. The place where Probe is bound is analyzed through autoradiograph and compared with master colony to find the exact segment of genome cloned in that colony.
Ma'am ... There's a question in my exam about two evidences which proves that dna replication is semi conservative. First exp was meseleson and Stahl exp but which one is the other ??? Pls reply .
Other one is experiments of Jhon Carins that proposed theta model (formation of theta shape in other bacterium) of DNA replication. One more is During the 1960s, Reiji and Tsuneko Okazaki conducted experiments in E. Coli to prove DNA replication is semi discontinous
@deepanshi please visit website contact us section. I suggest you contact through email or phone on your earlier querry regarding biotechnology carrier opp.
Hybridization the probes with genomic library....that step can you please elaborate...
Here in this step we perform colony Hybridizarion technique used for screening of genomic library.
In this step the bacterial colony is transfered to nylon membrane and denatured to expose the naked DNA . Then this membrane is soaked in a solution containing the labelled probe. Then washing is performed to remove nonspecific binding. The nylon sheet is sandwitched on Xray film. The place where Probe is bound is analyzed through autoradiograph and compared with master colony to find the exact segment of genome cloned in that colony.
@@BiotechnologyLessonsbyDrShaifa thank you that helps...👍
@@suhashg8664 very good question
@@BiotechnologyLessonsbyDrShaifa Ma'am contig assembly pr video pls
I tried to understand from many other videos but I got to understood correctly from your video. Thank you so much ma'am♥️🙏🏻
Thanku so much mam
Your teaching techniques are awesome
This was my demand ✋
Thank you so much Mam 😊
Also Share with your friends.
@@BiotechnologyLessonsbyDrShaifa sure Mam 😊
Brilliant way
Thank you. It was so helpful.
Kindly make the note available as pdf by scanning it on Adobe Scan (Light Text). Very good explanation. Helpful for exam purpose.
how we do sequence unknown dna and know about bases by this walking technique???
Chromosome walking is not a sequencing technique.Only restriction mapping is performed to identify subsequent marker gene
Thanks you mam , mam if you make a video growth curve and fungi details .. very helpful for us .
Thank you so much mam 👍👍
Thanks you ma'am
Nice explanation
Requesting u a video on chromosome jumping in near future.
Thanks
Thank you.
Mam plz upload reverse transcription and it's application
Very clear explanation
If you put subtitles means you will gain more subscribers and viewers because you notes are clear so please put subtitles
Dear jeeva video noes are already in english and am explaining them in Hindi. You mean subtirles in other language
Super mam, really good explanation, no one has explained it so well
Please also check in description box a small point is explained there regarding hybridization of probe
Thank you.. ❤
Maam please.. make new videos on bsc biotechnology topics please maaam
Mam can u please upload video Bacteriophage, structure and reproduction of a typical bacterium
Mam before that i purchase the notes of some other topic and i also pay the amount but u r not send me the notes why mam?
please contact me through email.I will surely send if missed.
Mam can you please add a video on metagenomics or microbial genomics??
Mam I request you to Plz make a video on chromosome jumping 🙏
OK will make it soon
Mam can you explain population genetic topic &hardy-weinberg law
Sure
Ma'am ... There's a question in my exam about two evidences which proves that dna replication is semi conservative.
First exp was meseleson and Stahl exp but which one is the other ??? Pls reply .
Other one is experiments of Jhon Carins that proposed theta model (formation of theta shape in other bacterium) of DNA replication.
One more is During the 1960s, Reiji and Tsuneko Okazaki conducted experiments in E. Coli to prove DNA replication is semi discontinous
@@BiotechnologyLessonsbyDrShaifa and ma'am ...exp by Taylor on vicia faba(broad bean) root cells...... This one is also fine na ?
Oh yes! Taylor experiment(1958) is more appropriate answer. as it proves that DNA replication is semiconservative.
@@BiotechnologyLessonsbyDrShaifa thanqq ma'am ☺️❤️
@deepanshi please visit website contact us section. I suggest you contact through email or phone on your earlier querry regarding biotechnology carrier opp.
Super mam
Mam...can u make a videos on "prokaryotic gene expression"...very important topic...
Mam after the partial digesion , are the fragments cloned and transformed to get the plaques or direct sequencing will be done?
Sequencing is done after cloning
Ma'am , does rna damage and rna repair mechanisms are different than dna ?
Yes it is different
I'm unable to download ur chromosome walking notes through ur website.. can u please help me
Please contact through email given in website
Mam can u please make a video on host parasite interaction for msc (notes)
You can visit biotechnology notes channel for notes related to biotechnology M.Sc , B.Sc
Good
Ma'am dna fingerprintung ka lecture provide kr dijeai
Maam can you make videos on molecular markers, pease
Already made a video on this topic
th-cam.com/video/O6flqEf6IE8/w-d-xo.html
Mam sabhi topic ki notes mail ya whts app pe milegi kya
Aap notes videos se dekh ke bana sakte h
Or
Aap notes purchase kar sakte h notes purchase karne ka website link description box mai mention h
Hello ma'am. Do you teach at a University or college?
Please see the aboutus section in website.
Mam I demand for genetic population &hardy weinberg equation plzz mam make a vedio on it soon plz
Will upload in 3 days.
@@BiotechnologyLessonsbyDrShaifa ok mam😊 thankyou soo muchh
Hello mam. Please make a video on chromosomal jumping.
Mam coromosome jumping & leaping par vhi video bna dijiye please please mammmmmmm 🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏
mam please make a video on chromosome jumping.....
Ma'am please do provide deletion mapping part with notes
Ky chromosome walking and chromosome jumping same h ??? please reply soon ma'm
No it is related but not exactly same
@@BiotechnologyLessonsbyDrShaifa kindly upload the seperate video on chromosome jumping 🥰🙏
Mam I have exam so I needs all biotechnology notes can you please share notes pdf I will pay the suitable amount.
please contact through email id given in website
Mam I have dropped msg please reply.
Thanks
Maam please aap aur video bnaao...
I am sorry but I don't understand a single word of your english
Thank you so much Mam 💓 💗
Thank you ma'am.
Thank you