Gene Cloning technique Part -1 | Steps involved in gene cloning Explained | In Hindi
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- เผยแพร่เมื่อ 1 ต.ค. 2024
- In this video I have discussed about what is gene cloning in Hindi, what are the steps involved in gene cloning.
In molecular biology, cloning a gene means inserting the sequence of the gene into a plasmid, which is a small piece of circular DNA that can be "transfected" into a bacterial strain, where the plasmid will replicate to many, many copies. The plasmid carries an antibiotic resistance gene, so when the bacteria are grown in the presence of the antibiotic, only those bacterial cells with the plasmid in them, can multiply.
This allows molecular biologists to easily propagate and grow vast amounts if the gene, and also to manipulate it in many ways, for example to introduce mutations to study its function, etc.
Please watch this video for more detail. If you like this video please like and share this video, and don’t forget to subscribe and click on bell icon for new videos.
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Mam you've a sophisticated personality and quite easy method of teaching ,love it from Pakistan ❤️
No doubt💫
Love 4 u from 🇮🇳
Mam ne to acha samjhaya but I love India
And I love Pakistan girls
@@vikasvishwakarma5641😂😂😂 desperate
ohh maam thank you so much the people like you save our education system
Allah apko seht de apiiii...
Bht bht shukriya....
The way of ur teaching is just incredible ,ur video is always helpful for us
Mam I have a doubt!!!!
How do we know whether the vector is cloning vector or expression vector??(
Thank you mam but 12 bord ke liya kya study kar sakte hai
Mam the gene responsible for the production of insulin woh gene hamare genome mey ek hi bar present hota ya multiple times hota hey?
I end up being confused reading this topic bt when i gotta knw abt uh ,U make me understand so well thanks alot
Plz mam Gene cloning ke process points bataye
Ek baat smjh ni aayi ma'am resistant gene agar vector ke paas hai to wo non transformant ko v survive kara le jayega?
Fir hme transformant kon h kon ni iska pta kse lagega?
Maam please explain cDNA synthesis..
Ma'am, how can we separate the fragment of DNA which has Gene of interest, from the other fragments? Is it done by gel electrophoresis?(I am referring to the topic taught at 4:40 in this video
Yeah by gel electrophoresis...
As we know that DNA having -ve charge so we put in as electrical field within a medium of agrose and strain with dye i.e. ethidium bromide and it visible only by passing it by UV rays so the DNA fragments (small) were goes towards the Anode and (Large ) fragments were at the cathode..
Very helpful mam i cleared my 7 doubts here...😀
Mam, jis plasmid me Gene of interest insert hua hoga wo Antibiotic resistance guma Chuka hoga(e.g. ampicillin) Aur jab Ham Sab bacteria Ko Ampicillin me rakhe ge to Recombinant wala to mar jayega to Uska Fayda kya jabki Non Recombinant plasmid , Jisme Gene of interest Insert nahi hua Wo to Dono Antibiotic me grow karega (As in NCERT Book) to hum kese Recombinant plasmid wale bacteria Ko Agal kar sakte he?
i liked your teaching .really helpful
Thanks Nikhil 😊
Mam your all video lecture very helpful for us ....Thank you so much mam ...
Mam Aap DNA damage and repair par bhi video lecture upload krdo mam please...
Thank you so much ma'am
Thanks 😊 maam teacher is best friend life ❤️❤️❤️♥️♥️ .my best friend teacher Khan sir and Ma'am you.❤️♥️♥️👏👍
U r great person 🙏🏼🙏🏼🙏🏼🙏🏼🙏🏼
Nice lesson
Very nice lecture mam please upload new video of genetics
Method of conveying is so inspiring so pretty. 👌👌
Mam...ye gene cloning and patent of life from k...video share kariye gaa na...MSc ke liye
Thank you mam ❤️
Mam please make video on charge relay system
good morning mam mam Mujhe titration ke bare mein Jankari chahiye and aapki mail ID ya contact number mile to achi baat hai
i've downloaded your videos... i recommend to others also...
your explanation is too good.....
Is possibile cloning human skin bodyparts for cure people?tx peace
Maaam you got me you have expained it in a very bes way
Love you maam u r a best teacher thq
the beauty of teaching , very intresting presentation
Amazing explanation👌👌very helpful🙂
Madam some bacteria can't live in high temperature....
Ms u r great
Mam
Why using antibiotic in agar plate
Antibiotics killed bacteria or not
Kidney liver ka cloning kyun nhi ho sakta for transplant
Agar is only used to solidify. It is not a nutrient enrich. Please clarify this.
Sir meri tablet pe sab videos chalte hai par apke is video ne mujhe bohat tang keya.
In case of self replicating vector...why there is no antibiotic resistance gene.
THNX MAM🙏🙏🙏🙏🙏
You use English words a lot that's why we didn't understand.
Maam upload more video genetic engineering pr please....tq so much
M'm please make a video about interfering gene interaction.
Mam my college professor use your video lecture series.
Jin sa vo hma pda rha ha
really very good expansion mam
aap bhut acche tarike se smjate h topic completely clear ho jata h👍😊😊
Thank You Devkaran 😊
Is video ko dekhne ke baad 90% samajh me a gya ki DNA cloning kya hai
Nice vdo mam tysm so much to Make in hindi version
Your video is very helpful for biotechnologies students
Maam, hume kaise pata chalega ki DNA me kaha pe hamara gene of intrest hai???
Mam but why it's important to add antibiotics in agar plate..
Important topic for upsc CSE
Thank you mam 🙏🙏
your way of teaching is good i m a universty student and follows you not my professors
Thank you ma'am it was really helpful 😊.
Mam aap genetic engineering topic se or video upload kijiye n
Some people come here just to see you😁😁😁 you are beautiful ❤️
Very helpful mam ...thanku♥️so much
Thanks to provide the quality of knowledge
मैम आप बहुत आकर्षक हो.. ❤️
Any 2020- 2021 batch here ...?😊
Don't know bt yh topic uss trah se ni smjaa paaye jause ki baaki k 😐
Very good explanation and you looking beautiful 😘
Do you believe in god
Mam, please explain about pUC19 plasmid
Blotting technique like
Southern
Norther
Western plz
Already there..please search in video list
Madam what is clave vector that was put in tube....
Clear & Crisp understanding..
Some new understanding I have learnt..Thank you so much.😊
Please Mam Make a video On NGS thank You 😊
Thank you so sooo muchh ma'am ❤❤❤
Most welcome 😊
Kangana ranavat jesi awaj Hai yaar but bhaut Acha smj Aya
Very nice thank you mam 😊. I also work as a lecturer it helped me a lot
Thanks Madhura
Awesome explanation
May u live long
Thx mam this video was very helpful for me
Ampicillin k Gene PR ampicillin antibody ka koi effect nhi padega?
Pr ampicillin dalne se Gene of interest ko Kya benefit milega
Ampicillin ka role mam clr nhi hua pls clr out this M confused...
All is good & clr
Hi Arshi.. mam ka mtlb he agr hm antibiotics use krte Hain to koi aur contaminated organism ko grow nhi krne dega hame Jo Gene chahiye wahi sirf grow hoga bina kisi rukawat ke is tarah antibiotics dusre unwanted organism ko grow hone se prevent karega
@@smriti220 ok miss. Smriti
& Thanks for this 😊
Very well explained mam..... Thank you mam
Cacl2 heat shock treatment
Mam biotechnology ki bhi video bana dejiye
Mam i understood but i want definition of gene cloning
Waaahh mam kyaa explaination tha😭😭🤍🤍🤍thankssss
Very nice lecture.I am understand very easily through this lecture on gene cloning.i appreciate you that make this type of Lectures much more
Clonning vector and expression vectors
Maam genetic ka video upload kar dijiye
Oh madam we need to do pcr to select our gene of interest
Mam,why we have to cLone? Directly can we introduce the insulin gene in the body?
Itna easy way me padhte hai maam koi nhi padhaya hoga mughe
Mam apne toh itna smjha kya bolu apko thanks❤
Thanku somuchhhh mam...cleared every point...very helpful vodeo👍💯🙂🙂
Mam is that RDNA technology
Love you from Afghanistan
Thanku so much ma'am....very helpful and easy to understand.
Really yrr such a helpful
Hi ! Kindly make brief vedio tutorial on mutations from genetics ..... Infact Ur conveying style is super .💞
Plz within 2days ☺️☺️
So informative & easy to understand in our own soft language.
May GOD bless you always.
May you please share your email or Whatsapp to ask some question if any, definitely I can never reach your office.
Only start to end lecture on gene cloning...
👌
Very nice presentation of information 😄
Where We introduce the gene To be clone in a vector?
U made that topic understandable at a drop of a hat much obliged ❤
10:40 Agar itself dont have any nutrient , you have to prepare LB(other medium)+agar+ antibiotics for this
Mam protein structure k uper videos banaye naa plzzz 🙏🙏i really need this video
Sure riki
Pls make video on protein microarray