Thank you so much for these videos! I have spent so much time trying to understand these concepts and you have explained them so well and so succinctly. I would love a video going through how all the components of the orbitrap MS work together in the diagram that you show at the end. I tried making sense of it myself and it's been a struggle...
Dr. Alan Doucette! It is so good to see you again! I am the student who took one of your class in Dalhousie university in Fall 2018 :) I also really enjoyed this video and it helped me really a lot, too! Thank you so much!
I have another question, I am doing research on the implementation principle of orbitrap mass spectrometer instruments.It is said that the signal generated from the orbital trap is very weak.Does anyone know how weak it is? Whether it's a PA level or a fa level or higher, does anyone know about this?. And I also want to know the frequency range of the signal of the detected ions. For example, if I want to measure an ion with a mass-to-charge ratio of 50-1000, what would be its frequency range? How big is the current? I'm an electronic engineering student and I'm trying to design a conditioning circuit for an orbitrap mass spectrometer
If you look closely at the outer "box" that encloses the central spindle, you will notice that it is divided in two pieces, left vs right. Each side has an applied voltage, that repells the ions. They swing back and forth in that box, while spinning around the axis.
I have a question ,what does the teacher mean when he say"If it was one continuous box, then you couldn't get the difference from one side to the other" in 10:42,Are there two channel current signals in the system or one?I saw it in a paper that We ampifier it with a differential amplifier，so i wonder are there two chanel signals of the same size but opposite phases?I am confused
While ions are orbiting around the central spindle, that motion is not directly connected to the image current we want to measure. To correlate with m/z, we are measuring the left-right motion. As the positive ions move to one side of the box (the orbitrap), they attract electrons onto that side of the box. Then, the ions move to the other side of the box and electrons follow, with a frequency characteristic of the m/z. You are correct that this signal must be amplified to be measured. And it is just that one main signal we are interested in.
@@alandoucette9997 In addition to direct amplification both modern ICR and Orbitrap instruments use differential amplification to minimize the impact of environmental frequency sources that can be removed by low noise and high common mode rejection ratio preamplifiers in well balanced detection circuits.
The easy answer is that the instrument already figures that our for you. The longer answer is that the instrument can be calibrated. So you run a set of compounds with know masses, and their measured frequencies correspond to those masses. The complicated answer relates to a whole lot of math, called a Fourier Transform. It's like how thousands of people can be accessing signals beaming through the air from a cell phone tower, and somehow turn all that "noise" into data. These are all frequencies, and yet "magically" our little pocket computers can make sense of them.
Resolution has no units. It can be defined in different ways, but most often it is the mass (m/z actually) divided by the width of the peak (at half its height). So the skinnier the peak, the higher the resolution.
Your explanation of complex mass spectrometry topics is quite remarkable.
Excellent summary! Thank you!
Thank you so much for these videos! I have spent so much time trying to understand these concepts and you have explained them so well and so succinctly. I would love a video going through how all the components of the orbitrap MS work together in the diagram that you show at the end. I tried making sense of it myself and it's been a struggle...
You made it so simple sir!!! Its just perfect for beginners. Thanks a lottttt.
Not the hero we deserve, but the hero we all need. Thank you very much for your videos, they make my studying much easier. God bless you.
Thank you, sir. The simplest explanation with an ample information
Dr. Alan Doucette! It is so good to see you again! I am the student who took one of your class in Dalhousie university in Fall 2018 :) I also really enjoyed this video and it helped me really a lot, too! Thank you so much!
I applaud you sir! Fantastic lecture!
Well explained and a wonderful instrument. Thank you! 👏
Sir Very beautifully explained.... Presentation matter is very easy to understand.. Thanks..
You have the best channel
I love you, sir.
Impressive thank you
amazing song choice, Kenneth ;)
That was amazing. Thank you.
You are a great teacher!
Wow, such a didactically nice video!
thanks for the info. Really needed that!
I love your channel
underrated channel!
Nice video - just a note however that it is based on a Kingdon trap not a Kingdom trap.
I have another question, I am doing research on the implementation principle of orbitrap mass spectrometer instruments.It is said that the signal generated from the orbital trap is very weak.Does anyone know how weak it is? Whether it's a PA level or a fa level or higher, does anyone know about this?. And I also want to know the frequency range of the signal of the detected ions. For example, if I want to measure an ion with a mass-to-charge ratio of 50-1000, what would be its frequency range? How big is the current? I'm an electronic engineering student and I'm trying to design a conditioning circuit for an orbitrap mass spectrometer
One small mistake: *Kingdon trap. Overall great video for education!
I want to take a class from you.
Nice
Why do the ions move back and forth while orbiting the central electrode in the orbitrap? Please answer. Thanks in advance
If you look closely at the outer "box" that encloses the central spindle, you will notice that it is divided in two pieces, left vs right. Each side has an applied voltage, that repells the ions. They swing back and forth in that box, while spinning around the axis.
@@shortchemistry7927 thanks for the reply
I have a question ,what does the teacher mean when he say"If it was one continuous box, then you couldn't get the difference from one side to the other" in 10:42,Are there two channel current signals in the system or one?I saw it in a paper that We ampifier it with a differential amplifier，so i wonder are there two chanel signals of the same size but opposite phases?I am confused
While ions are orbiting around the central spindle, that motion is not directly connected to the image current we want to measure. To correlate with m/z, we are measuring the left-right motion. As the positive ions move to one side of the box (the orbitrap), they attract electrons onto that side of the box. Then, the ions move to the other side of the box and electrons follow, with a frequency characteristic of the m/z. You are correct that this signal must be amplified to be measured. And it is just that one main signal we are interested in.
@@alandoucette9997 In addition to direct amplification both modern ICR and Orbitrap instruments use differential amplification to minimize the impact of environmental frequency sources that can be removed by low noise and high common mode rejection ratio preamplifiers in well balanced detection circuits.
Love the video but how do i translate my frequency spectrum into m/z spectrum?
The easy answer is that the instrument already figures that our for you. The longer answer is that the instrument can be calibrated. So you run a set of compounds with know masses, and their measured frequencies correspond to those masses. The complicated answer relates to a whole lot of math, called a Fourier Transform. It's like how thousands of people can be accessing signals beaming through the air from a cell phone tower, and somehow turn all that "noise" into data. These are all frequencies, and yet "magically" our little pocket computers can make sense of them.
6:30 when he says resolution above 2 million, what does that mean, what are the units of the resolution here
Resolution has no units. It can be defined in different ways, but most often it is the mass (m/z actually) divided by the width of the peak (at half its height). So the skinnier the peak, the higher the resolution.