Nick translation

[@akulajahnavi1043]

After every microbiology and biotechnology classes of mine, I will be searching for your videos. They are really worth of help.

[@shomusbiologyofficial]

+akula jahnavi glad to hear that

[@raisagrech3819]

your explanation is as good as gold and I might actually binge-watch all your videos before my exam xD

[@shomusbiologyofficial]

Thabk you so much for appreciating my efforts

[@silviamuccioli1907]

Your explanation was extremely useful! Thank you so much from Italy, and thanks to youtube for helping in spreading science across the world :D

[@sjonkers96]

I saw your face so many times today,

[@shomusbiologyofficial]

+Sven Jonkers thank you. Glad you liked my lectures

[@karass5616]

You "saved" my life ... THANK YOU VERY MUCH..i am from Algeria and all of my studies are in Frensh but u made it so simple ... once again thank you so much for making this video 😊🤗

[@shomusbiologyofficial]

+kara ss glad to hear that. Please subscribe and share

[@reshmirajeev5770]

Thankyou..sir..you will reach more heights..hands off to your efforts..🙇🙇

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@aboutstudies123]

Thank you very much Sir. Your lecture is one of the Nature's gifts.

[@shomusbiologyofficial]

+Bharath Raj that's a huge complement. Thank you

[@hodannuuh1254]

Your explanation makes molecular biology easy ❤️❤️❤️❤️❤️ thanks sir

[@joeljames9331]

You are awsum as usual ...i am an asst professor in an institute ...ur lectures are really helpful ...thanks :)

[@shomusbiologyofficial]

+Joel James thank you. Glad you liked my lectures

[@loelanto7841]

Thank you sir 🙏
Most helpful in this pandemic situation... 🙏🙏

[@shomusbiologyofficial]

You're welcome

[@morganeb4100]

Thanks to you for this video. Il have a question : How many nucleotides have been removed by DNAase?

[@kedargokhale7976]

Thanks! It was extremely helpful.

[@shomusbiologyofficial]

+Kedar Gokhale thank you.

[@pratitidas]

Very streight forward explanation.. thank you

[@fRdeady]

clearly explained, thank you

[@shomusbiologyofficial]

You're welcome

[@achorjoyce8031]

Thank you so much for sharing! This is amazing!!!

[@shomusbiologyofficial]

You're welcome

[@kitsukara6911]

Explanations are very clear! Thanks a million!

[@meghadhiman7944]

So helpful, Sir! Thanks much! Pls Sir tell me something about primer extension and end labelling.. I want these two topics..

[@pradeepsai7256]

Ok

[@shummu1458]

great explanation by great teacher👍👍

[@shomusbiologyofficial]

+Sumakshu Ghodeswar thank you.

[@raztarak]

Very good explanation, helped a lot

[@tammi55]

How does the polymerase know where to stop? Or does is only insert the radiolabled nucleotides "forever"?

[@parthaprotimchakraborty3225]

many many thanks bro ...nice explantn of those videos are properly clear my views ,, & really help me in my study .

[@heerachoudhary2885]

Aap bhut achha pdhate hh
So thank u

[@shomusbiologyofficial]

You're welcome

[@FlorMattheeuws]

Thank you, this was very helpfull.

[@vedikasarda4602]

But why ìs this technique called 'translation'? .. It could be named insertion or replacement. The definition of translation is different right? .. Could you please clear my doubt.

[@vedikasarda4602]

Or incorporation.

[@purvishrivastava54]

you are amazing!! Its very easy to learn now, Thankyou.

[@shomusbiologyofficial]

+Purvi Shrivastava you're welcome

[@assiadj7450]

tell me if its right or no plzz
the pol 1 will fix on the 3' side of that nick and after with his exonucleasic activity 5'-3' that enzyme will delete nucleatides and in the same time with his polymerase activity 5'-3' will add labeled nucleotids ???!! but the nick stay or not??

[@mahimasharma8647]

Could you please make a tutorial on Primer Extension Method -a radiolabelling method

[@asrujitdebnath2434]

plz post a video on RNA interferance

[@ghozlanebouzidi138]

Thanks, helpful explication !

[@bipashamondal8279]

Why the ad?

[@hajeranishath7729]

Very nice 👍

[@shomusbiologyofficial]

Thank you

[@priyankasaha407]

very much helpful... Thank U Sir...

[@Pooja_Rrrrr]

Thank you ... this is very helpful....

[@anadiroy6791]

Nice explanation

[@tsmeee]

you are a life saver bro

[@shomusbiologyofficial]

+Aimen Bouch thank you.

[@dextrannanoparticle8754]

It would be even more perfect if the video was subtitled

[@andiswantombela4358]

literally my Molecular medicine lecturer

[@shomusbiologyofficial]

You're welcome

[@zizihassan3437]

great extremely helpfulll thanks

[@akashsrivastava2842]

what is the purpose for adding DNA pol I again?

[@intensivegamer879]

Thank u so much sir👌👌.... sir can u cover end labelling of dna and RNA labelling in upcoming videos.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@faceyuyu12]

Thank you so much! So clear and useful!

[@ddplevel3866]

I'm Really thankful bro

[@shomusbiologyofficial]

+DDP Level3 thank you. Glad it helped

[@katielui131]

Hi, can you teach me how radioisotope S-35 is incorporated in the DNA molecule also acting as a radioactive marker? (coz I only understand how P-32 can be incorporated as it can be included in the phosphote group of the nucleotide) Thank you!

[@Overlyblankhead]

shomu's biology serves as internet tutorial for biology students.It helps graduates.

[@steveosazuwa2710]

Simple and Clean

[@vivimekairi]

is the way that you're making me feel tonight, it's hard to let it go.

[@raunakbanerjee3345]

ekta video korbe on aqueous two phase partition,please.

[@Kristina-iw4mf]

I had a little difficulty understanding because of your accent but the explanation is really very good. thank you!

[@sun65951]

when it comes to Dna replication ,it should be RNase H in the first step?

[@farhanaahmed7163]

Really helpful, thanks!

[@Jyotishmandevnath]

very helpful..thanks

[@ayamostafa5857]

very helpful thank you

[@theawestwater-smith7712]

thank you! all your videos are great! really clear and helpful :)

[@shomusbiologyofficial]

+Thea Westwater-Smith glad I can help

[@normanayal7459]

Thea Westwater-Smith

[@Prasino-Mauro]

Great video thanks a lot !!
I just have a simple question : if all the added dNTPs are radiolabeled wont that be problematic for the geometry of the molecule?

[@TheStarsky2011]

Crimson Jagger Geometry of a molecule is determined by its electron cloud distribution. Isotopes, including radioisotopes have the same electronic configurations. Decaying of the radioisotope may cause the release of alpha and beta particles which might cause damage to some of the DNA molecules.

[@sandradelfin397]

Thank u forever :) !

[@shomusbiologyofficial]

You're welcome 😊

[@Swarup_32133]

Why this is called translation?

[@priyankasaha407]

Thank you sir

[@JMSouchak]

Awesome video you're great!

[@shomusbiologyofficial]

+Joseph Souchak thank you. Glad you liked my lectures

[@stsfyt]

what happened in 5:48

[@mitylene_bailey]

Burp!

[@kawterfarid8465]

very very helpful thanks

[@shomusbiologyofficial]

+Kawter Kouki glad to hear that

[@monikan3347]

really helpful

[@sarrarhouma9336]

very helpful

[@mushroomfeast]

thanks
nice explanation

[@shomusbiologyofficial]

Thank you

[@MsJAM123456789]

why cannot restriction enzymes be used to cut the DNA?

[@gurmindersingh3684]

Janice Azzopardi because restrictions enzymes remove specific sequences whereas dnase1 creates a nick

[@elalamifatimazahra5716]

Thank you !

[@fairouz.7832]

Thank you

[@manelnoula6449]

From algeria ✌ thank u

[@AnkushSharma-zv5hv]

why is the strand not completely degraded ???

[@TheStarsky2011]

Ankush Sharma Because DNase 1 is an endonuclease. It cuts within the DNA molecule. Nick thus created forms the site for nick translation initiation.

[@engyelekhnawy9170]

Thanks alot

[@heerachoudhary2885]

Plzz make this video in hindi
Plzzzz
🙏🙏🙏

[@shomusbiologyofficial]

Sure

[@medinisamant2962]

Don't the normal dNTPs get bound again due to polymerase activity?

[@TheStarsky2011]

Medini Samant Degradation of DNA strand by DNA pol 1 creates dNMPs. Unless added externally, there are no normal dNTPs to polymerise

[@nillay]

Awesome thank you!!

[@alenaderiabina293]

Thank you!!!

[@kanikakaur3888]

very helpful, but very lengthy

[@shomusbiologyofficial]

+Kanika Kaur it's lengthy because it is detailed

[@theshubham310]

Thank You So much foe the explanation! :)
I can see that you know your biology.I would just like to advice you to improve your body language while explaining,I think it will help you in the future! :D

[@faryalbabar9020]

amazing....

[@arjunbharati7675]

thanks

[@Ssc_26x]

You're genius :)

[@shomusbiologyofficial]

Thank you

[@rimnasamanta7772]

thnx boss ....

[@divyaswaroop9203]

Ty sir...

[@unboxing0021]

Pakistan loves you.

[@shomusbiologyofficial]

Thank you

[@RitiSharma-v7d]

O bhaiii Hindi v use kr liya kr

[@shomusbiologyofficial]

Hmm

[@bhaskarthakur1068]

Thank you

[@shomusbiologyofficial]

You're welcome

[@andiswantombela4358]

thank you so much

[@shomusbiologyofficial]

You're welcome

[@mehulpatel1572]

Thank you sir

[@shomusbiologyofficial]

You're welcome

[@kokokisser]

Thank you!!!

[@serafinamarzocchella3043]

Thank you

[@shomusbiologyofficial]

You're welcome

[@farahnakachi4759]

thank you

[@shomusbiologyofficial]

You're welcome