So a laser beam illuminates specimens pinpoint target on or below surface of the specimen. The target area is scanned rapidly across a few cell thick radius at a time. Different focal planes and depths allow for a 3D to be made on real-time from the signals back from the light collected. The confocal microscope has a high resolution, high magnification, it can also be used for whole specimens which are living without being invasive - it must however be fluorescent which can cause artefacts
I did a google search for "confocal microscope image," and saw the image of an embryo stained to show DNA and microtubules that is shown ~50 seconds into this video. I immediately HAD to follow the link, because I made that image when I was in grad school! Funny coincidence.
So a laser beam illuminates specimens pinpoint target on or below surface of the specimen. The target area is scanned rapidly across a few cell thick radius at a time. Different focal planes and depths allow for a 3D to be made on real-time from the signals back from the light collected. The confocal microscope has a high resolution, high magnification, it can also be used for whole specimens which are living without being invasive - it must however be fluorescent which can cause artefacts
thank you so much. Perfectly described in just about two minutes!
Very instructive, thank you
I did a google search for "confocal microscope image," and saw the image of an embryo stained to show DNA and microtubules that is shown ~50 seconds into this video. I immediately HAD to follow the link, because I made that image when I was in grad school! Funny coincidence.
Gracias por la explicación