Hello, thank you for this video. I had one question: in the case of doing a semi log XY graph of log10 ligand concentration on the X axis and specific binding on the Y axis, how can I interpret the data on the graph by finding the Bmax value by seeing where the max X value intercepts on the Y axis, and from this calculating the Kd constant through looking at the 50% occupancy value?? Thank you!
Hello, thank you for this video. I had one question: in the case of doing a semi log XY graph of log10 ligand concentration on the X axis and specific binding on the Y axis, how can I interpret the data on the graph by finding the Bmax value by seeing where the max X value intercepts on the Y axis, and from this calculating the Kd constant through looking at the 50% occupancy value?? Thank you!
I am not sure whether semi log is the right way to plot the data. It is unlike the Hill equation to calculate EC50.