Thank you very much, I'm french I revise this chapter intituled : "Beer Lambert Law" or in French : "La loi de Beer Lambert" and in order to develop in English level and enhance it, I would to look this video. It is very good explicated ! Thank you !
Thankyou teacher thanks alot for this great explanation I was fed up of my teacher's teaching But I love it and for the first time I'm feeling confident that now I can explain and wite it Please make more and more videos UV spectrophotometer IR spectrophotometer Fluorimetry and colorimetry and many more topics please I have likes and shared to all my classmates and also subscribed Thankyou sooo much teacher Thanks alot
Hello , thank you so much for your informative video , i am interested in measure absorbance for polluted water or water from river and lakes , can you pls tell me 1- how to convert reflectance to absorbance 2- how to correct absorbance and remove bad value, thanks again so much great teacher.
A spectrophotometer can work under both Beer's law and Lambert's law, as these laws describe different aspects of light absorption in a solution. Beer's law states that the absorbance of a solution is directly proportional to the concentration of the absorbing substance in the solution, as long as the path length of the light through the solution is constant. This law is often used to measure the concentration of a solute in a solution by measuring the absorbance of light at a specific wavelength. Lambert's law, on the other hand, states that the absorbance of a solution is proportional to the path length of the light through the solution, as well as the concentration of the absorbing substance. This law is used to calculate the amount of light absorbed by a solution with a known concentration and path length. In practice, spectrophotometers often use both laws in combination to accurately measure the concentration of a solute in a solution. The spectrophotometer measures the intensity of light passing through the solution at a particular wavelength, and then uses Beer's law to calculate the concentration of the solute in the solution. The path length of the light through the solution is also taken into account using Lambert's law to ensure accurate measurements.
Blank means solution without protein, baki sab me proteins ki different concentrations honge, lekin blank me nhi, then we drop the reagent which gives colour on reacting with proteins in the samples.
The excitation coefficient is another name for the molar extinction coefficient, which is a measure of how strongly a chemical species absorbs light at a given wavelength. It depends on the molecular structure and environment of the species, as well as the frequency of the light. The excitation coefficient can be experimentally determined by measuring the absorbance of a series of solutions with known concentrations and path lengths, and then plotting absorbance versus concentration. The slope of the linear regression line is equal to the excitation coefficient times the path length. Alternatively, if the excitation coefficient is known, the concentration of an unknown solution can be calculated from its absorbance and path length using the Beer-Lambert law. I hope this helps you understand the concept of excitation coefficient in Beer-Lambert law. 😊
Thanks. I wish our PhD teachers in the university had only one tenth of your teaching talent
Thank you very much, please share among friends if you found it helpful.
Wonderfully explained...Quiet thankful for your help and answering the questions arising in my mind..
Glad it was helpful!
Wow ,your tutorials is very interesting me so much and your sound also make me understand what you speak morre
great video! subscribed :D this helped me learn stuff for science olympiad. very thankful for this vid
Thank you Mam. Khub sundor explaination. Many many thanks.
Thanks a lot dear, please do share if you liked our video
Oh my God this video is so informative thanks I wish you could do more on spectroscopic methods please
More to come!
Many many thanks for your nice and effective presentation!!!!!
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Thank you very much, I'm french I revise this chapter intituled : "Beer Lambert Law" or in French : "La loi de Beer Lambert" and in order to develop in English level and enhance it, I would to look this video. It is very good explicated ! Thank you !
merci beaucoup pour votre geste d'amour. Je souhaite tout le meilleur, continuez à nous soutenir.
@@RethinkBiology merci j'ai pu enrichir en plus de ça plus de vocabulaire en anglais
Very good video...... thank you...
Thank you too
Really outstanding explanation
Thank you very much
you just grabbed my strong attention...awesome
Thank you ma'am for an best explanation video 😊🙏🏻.
You are most welcome 😊
Most underrated biology channel
You are doing a great job mam😇😍
Thank you very much. Be with us. 😊
Thankyou teacher thanks alot for this great explanation I was fed up of my teacher's teaching
But I love it and for the first time I'm feeling confident that now I can explain and wite it
Please make more and more videos
UV spectrophotometer
IR spectrophotometer
Fluorimetry and colorimetry and many more topics please
I have likes and shared to all my classmates and also subscribed
Thankyou sooo much teacher
Thanks alot
Thank you very much.. keep loving and supporting us... Will definitely try to bring videos on those topics... ❤️
Hello , thank you so much for your informative video , i am interested in measure absorbance for polluted water or water from river and lakes , can you pls tell me 1- how to convert reflectance to absorbance 2- how to correct absorbance and remove bad value, thanks again so much great teacher.
So helpful!!
Thanks a lot 🙏🏻🙏🏻
Thank you ☺️
Thank you so much 🙏🏼🙏🏼
Best video
Thanks a lot dear
Hello , can you pls preapare video how to correct Specta using filters
Very informative video ❤ it helps me a lot. Thank you ma'am😊.
Does value of absorption coefficient for a drug remain same in water ,as well as in n octanol at same wavelength?
Doubt to clear madam,
Spectrophotometer Works under Lambert's Law or Beer's Law - what is difference between them..
A spectrophotometer can work under both Beer's law and Lambert's law, as these laws describe different aspects of light absorption in a solution.
Beer's law states that the absorbance of a solution is directly proportional to the concentration of the absorbing substance in the solution, as long as the path length of the light through the solution is constant. This law is often used to measure the concentration of a solute in a solution by measuring the absorbance of light at a specific wavelength.
Lambert's law, on the other hand, states that the absorbance of a solution is proportional to the path length of the light through the solution, as well as the concentration of the absorbing substance. This law is used to calculate the amount of light absorbed by a solution with a known concentration and path length.
In practice, spectrophotometers often use both laws in combination to accurately measure the concentration of a solute in a solution. The spectrophotometer measures the intensity of light passing through the solution at a particular wavelength, and then uses Beer's law to calculate the concentration of the solute in the solution. The path length of the light through the solution is also taken into account using Lambert's law to ensure accurate measurements.
Hope this cleared your doubt
❤❤❤ thx you so much
Epsilon is the extinction coefficient..
(Not excitation ) 😁🙏🏻
Mam its so nice explanation 😊 it help me a lot❤
Blanks main pehly sy kya hota hain then we drop protein
Blank means solution without protein, baki sab me proteins ki different concentrations honge, lekin blank me nhi, then we drop the reagent which gives colour on reacting with proteins in the samples.
ma'am, I like ur content very much, but the background music is very annoying, we only want to hear ur voice.. if it possible thnq ma'am
Thank you so much for your feedback,will keep that in mind..😊
Glad it helped, please support us by inviting your biology loving friends to subscribe our channel. Thanks 😊
Your voice is very nice mam
Very well explained mam ,
Thank you mam its helps!
Thank you!
Nice explanation
Thanks
Plsss upload many videos in gpat and government exam point of view plssss
Of course, please suggest us some topics which you found difficult to understand.
Nice
thank u so much ........
Most welcome
Thank you
Thanks ❤
Thnxx mam
Welcome.. keep supporting us..😊
Thank you 😊
what is that about excitation coefficient? can u explain that part pls?
The excitation coefficient is another name for the molar extinction coefficient, which is a measure of how strongly a chemical species absorbs light at a given wavelength. It depends on the molecular structure and environment of the species, as well as the frequency of the light.
The excitation coefficient can be experimentally determined by measuring the absorbance of a series of solutions with known concentrations and path lengths, and then plotting absorbance versus concentration. The slope of the linear regression line is equal to the excitation coefficient times the path length. Alternatively, if the excitation coefficient is known, the concentration of an unknown solution can be calculated from its absorbance and path length using the Beer-Lambert law.
I hope this helps you understand the concept of excitation coefficient in Beer-Lambert law. 😊
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mam , can i get the video ??
❤❤❤❤❤❤
Background noise should be removed.
Take a breath and study
Ok 🤝
Let do both together?
@@ChandanKumar-cn9hs sure
@@kashmirapradhan6542 bsc ? Which year ?
@@ChandanKumar-cn9hs Ph.D acholar what about you ?
شرح حلو جدا
لكن بالعربي بيكون افضل علشان نفهم اكثر
ترجمة النص باللغة العربية إذا استطعت. يمكنني تزويدك بنص الفيديو. سيكون مفيدًا للعديد من الأشخاص مثلك.
Umesh chaudhari CBZ
those signs that says "subscribe" are so annoying, distracting. It kept showing up
👍 👍👍👍👍👍
Anyone for Rgpv Engineering chemistry 😂
Super explanation, but i felt like your voice is very over acting
Not much depth..
Will try to put a much depth version very soon
Tnq ma'am
Thank u mam