Absolutely love these videos! They are advanced for me but very clear! I did have a couple questions though. Does splicing happen before, during, or after polyadenylation? Also where exactly do the endonucleases cleave? You mentioned that after the cleavage the mRNA was freed from the DNA for the most part so is it almost like a helicase going down the middle to separate them? Or is it cleaving a small part after the AAUAAA sequence that allows polyadenylation to occur? Thanks so much!
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Absolutely love these videos! They are advanced for me but very clear! I did have a couple questions though. Does splicing happen before, during, or after polyadenylation? Also where exactly do the endonucleases cleave? You mentioned that after the cleavage the mRNA was freed from the DNA for the most part so is it almost like a helicase going down the middle to separate them? Or is it cleaving a small part after the AAUAAA sequence that allows polyadenylation to occur? Thanks so much!
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Always Hi not Bye Sir ❤
hey ! you didnt talk about CPSF ( cleavage and polyadenylation specificity factor ) and CSTF ( cleavage stimulation factor ) .
He said that in previous videos
hi sir plz let me know which topics are important from biochemistry and enzyme kinetics
Enzyme kinetics and enzyme inhibition
First view 🎉 first comment 😊
Thank you
Why only adenine residue is added in poly A tail, not guanine cytosine or urecil
because it is Poly A polymerase enzyme, simple polymerase can add all the nucleotides but poly A will only add Adenine
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Thank you