TAP media and agar preparation for culturing microalgae

Project: Production of Lutein from Microalge

[@p16able]

Very informative video! Do you get any bacterial contamination in your culture? If yes how do you take care of it ? Thanks.

[@setyawidhi1607]

hi, I am interested to learn about making a medium for culture algae. I have a question are we can sterilize with more than one-half hours in autoclave? thank you

[@chinmayeechhotaray7116]

Can you please describe the work of each chemicals used in the media prep?It would be great help for me.Thanks a lot

[@harshvshalihotri4787]

Will this media and agar be able to work to grow any strain of microalgae?

[@user-wx2ow8rm7x]

I can stir elements or give air only ??

[@truongvanhuyhoang6481]

Excuse me, can I ask you what the ingredients of the nutrient medium are in?

[@leoandkenofficial5423]

when doing agar medium, you added agar into TAP medium that is autoclaved before or not yet? Thank you so much.

[@syazein]

hello, may I know how to prepare the 85 mL of 20% potassium hydroxide? thank you!

[@bioproject2705]

sorry for the late reply.. you have to weigh 200 g KOH pellets and dissolve in 1000ml of distilled water ( or 20g KOH pellets dissolve in 100ml of distilled water)

[@syazein]

another question, why do we need the Hutner's trace elements?

[@bigblackmachine9533]

Is this the zarrouk medium for spirulina?

[@TheShreyansh999]

No. This is TAP medium for chlamydomonas

[@Akhirra]

hello sir, as a commoner i would like to ask for help what is the simple recipe for agar and broth to inoculate/culturing Purple Non Sulphur Bacteria(PNSB)? 🙏🏻

[@user-vv2to3hz7k]

ث بقص

[@aichazine9602]

I can contact you because I have more question

[@bioproject2705]

hi.. please email your question to us at [e-mail] maybe I can help you with the question.. sorry for the late reply..