Virology Lectures 2023 #2: The Infectious Cycle
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- เผยแพร่เมื่อ 23 ม.ค. 2023
- The complete course of events in a virus infected cell is called the infectious cycle. In this lecture we discuss the different phases of the infectious cycle and methods for studying it, including plaque assay, multiplicity of infection, and the particle-to-pfu ratio. Physical methods for detecting viruses include hemagglutination, serological techniques, polymerase chain reaction, and high-throughput sequencing. These methods were used to identify in early 2020 the pandemic coronavirus SARS-CoV-2.
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I [Nelson] express my gratitude for the opportunity of updating in virology, despite at a distance (Brazil). I started attending your courses in 2020 during the COVID-19 pandemic. Thank you professor Racaniello.
I’m so excited that Dr Racaniello is starting the 2023 update of his excellent lectures on virology. And this time, I will be following these as soon as they appear on TH-cam.
I'm with you! Good stuff!
Thank you so much for sharing this wonderful information. The illustrations make it easier to understand.
Thanks so much for these courses! Great review from my studies of some virology years ago (and some new info, too!)
I've been watching these lectures since 2020 when the class went remote halfway through. Thank you for making this available. There is so much mis-information around the infectious cycle it is great to have one place to go.
This is absolutely brilliant. Thank you for the opportunity to study with you.
This is great! One thing, I single-framed through part of the plaque growth video, and it seems to be hours, not days, based on the second set of numbers advancing 10 through 50 and then the first number advancing by 1 when the second set hits 00.
I was gonna take this course but seems I can learn it all without spending $6k and stressing over exams. Nice.
I have watched this course for several years. I recommend that you purchase the book "Principles of Virology" unfortunately I think it is out of print and a new edition is planned. Used copies are probably available online, eg; Ebay.
@@christopherrobinson7541 Thank you for the pointer!
awesome
@58:27 Coronavirus phylogenetic tree what is the number 63? All of the others have high numbers above 80, but for 2019 wiv02-wiv07 we see 63. Is that the correlation between the viruses?
Thinking about the mRNA vaccine, are there cells that are resistant? Are there cells that are susceptible? Are there cells that are permissive? Are there cells that are susceptible and permissive? OR are all nucleated cells susceptible and permissive and hence the mRNA can enter all cells equally and replicate equally?
@ 39:10, How do you do a plaque assay on celless viruses? What’s supposed to be visible?
So the infected cells secrete virus into the growth media, so you take some of the media minus cells and do a plaque assay. This tells you how much virus has been secreted by the infected cells.
thank you. i saw a movie about henrietta.
What if a cell type has two! receptors that the virus needs, like CD4 & CCR5 / CXCR4 with HIV? At 2:32 you speak of " a functional receptor", but sometimes as with HIV a functional receptor is not enough, it needs two. plural!
If you take 0.1ml out of a10-6 dilution tube, how does it become a 10-7 dilution without placing it more solution? How does the concentration change by one log? Why am I confused?
So the dilution doesn't change right? However, we solve for the amount of PFU's per *milliliter*. So, if we find that we have 15 viruses in 0.1mL of the 10^-6 dilution, then we can find the original concentration in PFU's per mL by 1. dividing the number of plaques by 0.1mL (same as multiplying by 10) and then 2. dividing by the dilution factor (10^-6).
@@anythreeletters Thank you!
Hemmagglutination possible for Type O ?
Hemagglutination is not blood type dependent.
Love the intro music
Shazam said it is "Moment" by Etwas Anders, I didn't listen to the whole thing, though, assume it's just a snippet.
I am anxious about displaying my ignorance but here goes: I don't get 45 from e^-10. what am I doing wrong? I get 4.539E-5
anybody please feel free to suggest direction for me. thank you
You have the right number don't worry, I think you've just missed that P(k) is a fraction. You need to multiply the total number of cells (10^6) by P(0) to find the number of cells receiving 0 particles : 10^6 x e^-10 is equal to ~45.
@@gingerbiscuits Thank you SO much!
I ve never saw a cell culture in a Petri dish
13:34
This intro music is so groovy 😎
"Probable bat origin" they wrote, probably knowing already what the exact origin was.
GFYS Vincent!
This antivaxxer didn't watch the lecture because he already knows he would not understand almost all of it and the few parts he did manage to understand would give the poor thing cognitive dissonance.
First