Dude, you lab looks grimy af. Maybe clean up? Also, you're making several mistakes that make your stain pretty worthless. Yes, it will stain the cells, but no, it will not conform to any standard and in result you will not be able to judge a number of characteristics correctly. Please don't use this for Malaria, for example! Here are some suggestions for improvement: - Don't use tap water! Use a buffer solution (Soerensen or Weise) at pH 6.8 for hematology and 7.2 for malaria. The uncontrolled pH and buffer capacity of your water will make the colors totally variable depending on lcation and time point! - Don't filter the stain after dilution. If it needs filtering, filter before. Filtering it as diluted liquid retains dye in the filter and shifts the color balance. -Fix with 100% methanol. Adding 30% water will just make the result worse. Many authors even claim that daily methanol change is required due to moisture uptake.
tap water is not supposed to be used ,you can use rain water to improvise because rain water is compared to pure water since it doesnot contain those minerals
Dude, you lab looks grimy af. Maybe clean up? Also, you're making several mistakes that make your stain pretty worthless. Yes, it will stain the cells, but no, it will not conform to any standard and in result you will not be able to judge a number of characteristics correctly. Please don't use this for Malaria, for example! Here are some suggestions for improvement: - Don't use tap water! Use a buffer solution (Soerensen or Weise) at pH 6.8 for hematology and 7.2 for malaria. The uncontrolled pH and buffer capacity of your water will make the colors totally variable depending on lcation and time point! - Don't filter the stain after dilution. If it needs filtering, filter before. Filtering it as diluted liquid retains dye in the filter and shifts the color balance. -Fix with 100% methanol. Adding 30% water will just make the result worse. Many authors even claim that daily methanol change is required due to moisture uptake.
How long can we reuse the absolute methanol, and how do we store it so we can use it for more longer time? And can we reuse giemsa 10% as well after first staining?
Thank you ❤️ it's helped me a lot during practical 😌
How to prepare gimsa solution ?
This guy is staining half his lab, aside from staining blood smears. That is one dirty lab.
طبعا احنه الصيدله ما نستفاد منه شي بس ميخالف نشوفه علمود الدكتوره فقط😂😍
Thanks
Thanks a lot❤
Dude, you lab looks grimy af. Maybe clean up? Also, you're making several mistakes that make your stain pretty worthless. Yes, it will stain the cells, but no, it will not conform to any standard and in result you will not be able to judge a number of characteristics correctly. Please don't use this for Malaria, for example!
Here are some suggestions for improvement:
- Don't use tap water! Use a buffer solution (Soerensen or Weise) at pH 6.8 for hematology and 7.2 for malaria. The uncontrolled pH and buffer capacity of your water will make the colors totally variable depending on lcation and time point!
- Don't filter the stain after dilution. If it needs filtering, filter before. Filtering it as diluted liquid retains dye in the filter and shifts the color balance.
-Fix with 100% methanol. Adding 30% water will just make the result worse. Many authors even claim that daily methanol change is required due to moisture uptake.
Can you please tell me the simple procedure 🙏
Step by step
Great, informative
keep it up👍
Mashallah sir G ❤
Sir do we need to put coverslip or not before adding the immersion oil
Thanks ❤
Like it continue posting
Thank you🙏
Amazing thanku
Apne blood kitna liya 10 microlitre ya 5 microlitre
Perfect
Very helpful. Thank you, Sir. Can tap water, of well origin, aka hard water, be used or could the minerals affect the results?
tap water is not supposed to be used ,you can use rain water to improvise because rain water is compared to pure water since it doesnot contain those minerals
That is not properly make by using correct procedure so plz give further detail how it is possible to make best slide without using buffer solution
1part kya lena hai stain ka name
Excellent
How long gimsa used
Amazing👍😍
Brother plz give more practical knowledge
Sir can I use 50 ul of giemsa stain and 450 ul distilled water for 1:10 dilution? Pls reply
50/500=1/10 so absolutely
Nice
Padhe ka vdo h ya shadi ka?itna nacha nacha k dikhaya h😅
Dude, you lab looks grimy af. Maybe clean up? Also, you're making several mistakes that make your stain pretty worthless. Yes, it will stain the cells, but no, it will not conform to any standard and in result you will not be able to judge a number of characteristics correctly. Please don't use this for Malaria, for example!
Here are some suggestions for improvement:
- Don't use tap water! Use a buffer solution (Soerensen or Weise) at pH 6.8 for hematology and 7.2 for malaria. The uncontrolled pH and buffer capacity of your water will make the colors totally variable depending on lcation and time point!
- Don't filter the stain after dilution. If it needs filtering, filter before. Filtering it as diluted liquid retains dye in the filter and shifts the color balance.
-Fix with 100% methanol. Adding 30% water will just make the result worse. Many authors even claim that daily methanol change is required due to moisture uptake.
How long can we reuse the absolute methanol, and how do we store it so we can use it for more longer time?
And can we reuse giemsa 10% as well after first staining?
yes, can anybody please give any thoughts on this question?
Thanks