The blood smears shouldn't extend to the edges of the slide as the white blood cells concentrate there. Also, make sure there is sufficient space to the narrow edge of the slide! Here, the blood volume was way too high.
Its my lessons at professional school,so incredible!!Hi from Greece,and thank u so much for the uploading!!I have to ask u ,about the last part of the video what amount of water we throw into acetonic ?
What is the purpose of Azure B in Giemsa stain? The methylene blue stains the nucleus blue/purple colour. Eosin Y stains the cytoplasm orange/pink, so what does Azure B stain?
As a med student in the biochem lab, I could never get the spread on the smear right. Always uneven.
Thank you this video help me to understand blood smear :)
Is there s any reference for using Wright First and Giemsa second!!..!?
why the sample is not completely washed away as we put the slide onto the water? is it the methanol holding the bloodcells?
it is fixed with dye, won't wash away with water.
Is the Wright and Giemsa stain undiluted one? And the distilled water pH?
is one staining method better than the other???
What did you fuse the slides together with?
I haven't got any methanol to fix with, will isopropanol work?
No. Methanol has to be of good quality and dry.
What percentage of methanol
Are you using?
100% and dried. Water and residues cause artifacts.
The blood smears shouldn't extend to the edges of the slide as the white blood cells concentrate there. Also, make sure there is sufficient space to the narrow edge of the slide! Here, the blood volume was way too high.
Amazing
Its my lessons at professional school,so incredible!!Hi from Greece,and thank u so much for the uploading!!I have to ask u ,about the last part of the video what amount of water we throw into acetonic ?
best method
How to do tzanc smear
What is the purpose of Azure B in Giemsa stain? The methylene blue stains the nucleus blue/purple colour. Eosin Y stains the cytoplasm orange/pink, so what does Azure B stain?
+vOzyv it stains acidic elements of the cell blue (nucleic acids, protein of the cell) since Azure B is a basic dye
Dyana D ..شكراااا على المعلومه
I wanted to dawlod this video
Great!
PS u never make a blood smear from the cap contents
Is that the DBZ game background? 😂😂
Is it completely necessary to put a coverslide on Giemsa stain? I know It is not for Wright, but I do not know for Giemsa.
buena
Thanke for you
اكو احد جاي يشوف الفيديو لو لبستوني كالعادة
nice
jcgax70 what is the meaning of giemsa stain.
I like
How to Sudan black b stain perform and how to vapour formalline fixation the leukaemia slide in this procedure