Please don't squeeze the finger while drawing capillary blood as the tissue fluid oozes out and mixes with the blood thus diluting the blood sample giving false results.
First i want to thank you a lot for this video, it helped me learn a lot! And secondly I want to ask, Why don't we put a cover slide on the specimen before putting it under the microscope?! Hope you answer me soon!
On doing DLC there is no need of using cover slide. Hence its oil immersion 100x lens is used. The lens should touch the oil After counting we have ti rub the tip of lens using spirited cotton
Lesmain dalne k 2 Min bad buffer solution 2-3 drop dalte h . Fir 5-10 min k bad use tap water se dhona h Uske bad tissue se sukha k use microscope k liye ready krte h
We dont have to rinse it completely with distilled water na otherwise the slide will be washed up completely and we will get negative results so its better to put the thumb on the slide so that it shouldn't rinse completely otherwise it will show wrong results
Distilled water is the pure form of water which is made heating , boiling, cooling vapours, that is a hypotonic solution but saline made my adding sodium chloride in water which is isotonic solution use for IV fluids.
Sir ek request hai . Meri slide may black deposit bahut Jyada aate hai.. .agar dobara stain dal kr wash krti hu to platelets nahi dikhte... Plzzz help me Mai kya kru.... Hmari doctor roz bahut Jyada dantti hai Muje... Plz help me 😥😥
Black deposit aane ka matlab stain puri tarah spred nhi ho rha ..dilute proper kro ...ek baar se doctor se hi puch lo ki sir kaise Krna h ek baat bta do
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Good Job sir 👏 ( Morning me mera practical exam Hai 😅)
Please don't squeeze the finger while drawing capillary blood as the tissue fluid oozes out and mixes with the blood thus diluting the blood sample giving false results.
Yaar lekin squeeze nahi karte to blood nikal ta hi nahi
@@_ABHISHEKSAHA_MBBSO- Uske liye needle 45 degrees angle par pakadkar 2 to 3 mm deep prick karo
@@tanmayeenalavade394 thanks, I will try it in my next hemat practical
Bilkul Correct bola apne
We have to do DLC not TLC or TEC
Boht acche se bataya shukriya 🖤😃👏🏽
First i want to thank you a lot for this video, it helped me learn a lot!
And secondly I want to ask,
Why don't we put a cover slide on the specimen before putting it under the microscope?!
Hope you answer me soon!
On doing DLC there is no need of using cover slide. Hence its oil immersion 100x lens is used. The lens should touch the oil
After counting we have ti rub the tip of lens using spirited cotton
Thanks sir... This help me alot🙏
First drop of blood is discarded right ✅
Thank you sir
Lesmain dalne k 2 Min bad buffer solution 2-3 drop dalte h . Fir 5-10 min k bad use tap water se dhona h Uske bad tissue se sukha k use microscope k liye ready krte h
Kaha se ho
Good 👍😊
Very Helpful video 🙋🙋
Great explain//.👍🏻
Thank you
Thank you for explaining
Thanks sir from Bihar🙏
My pleasure
@@MLTLabManual a
This method is very egy 😊
Thank you so much for your effort
You can mix it by blowing also
Why you are not adding cedarwood oil
No need
Sir we never take first drop of blood ....we have to wipe it
Yes true we have wipe the first drop
Why should we wipe it?
@@criminalgang6534 Because it contains tissue fluid
Thanks a lot 💓
No need of methanol fixation for Leshmin stain, its alcoholic stain
Yes
Right
Right
Just found your video... The best video.. Sir can you please explain how to use oil immersion.... Please😫🙏🙏
high power me dekhne ke liye...
normal lens se 100 times extra dikhta h oil me
Just take a drop of imersion oil on smear and watch it under a microscope 🔬 simple
M. P. Stain reagent A and b hota hai sir red or blue konsa use pehle hota hai btaye
Thank you bro 👍
Nyc explanation 😊
Sir hm smear 100x pe dekhte hai na 🙄
How to prevent rouleaux formation ??
Make thin smear to avoid Rouleuxe formation
Distilled water se ku tap water use kr sakte hai
Yes
Yes it can be with tap water
Sir.. can I take a short part from this video to make a lecture for kerala higher secondary examination?
Nice ayit 🤣
Nice presentation 🤩
We dont have to rinse it completely with distilled water na otherwise the slide will be washed up completely and we will get negative results so its better to put the thumb on the slide so that it shouldn't rinse completely otherwise it will show wrong results
Thanks for your feedback
Sir aap finger name galat bol rahe ho ek middle finger hai ek ring finger
Kya koi mujhy bata sakta hai ki kyu hum prick ring finger ya fir middle finger me karte hai baki fingers me kyu nhi. ???reason?
Bcz dusre me finger me blood ka flow thoda km hota hai
Metanol sei fix bhi karni hoti hai slide
Leishman me nhi krte hai, wo giemsa stain me krte hai
Thank you sir 😇🙏🏻
Patna में कोई Lab या teacher बताइये जो manual काम सिखाते हैं
No Idea
What is the color of Leucocyte and malarea and trypanosmoma
When we use the lieshmin stain?
Methanol kitna percent use karte hai sir
Tomorrow my exam thank god 😅
Best Wishes
Distilled water kitne drop daalna hai wo bhi bataaea
Leishman stain ke double volume
Good explanation bro
Hai yarrrr krta to haii😂😂
Maanta h ki nhi
Thanks form Pakistan ❤
Nicely explain
Thank you very much sir ji
My pleasure dear
Index finger he ku use krte h prick ke liye hmesha ...!!!
Thank you so much sir
Thanku so much sir🤗
Ek bar ye bataiye ki slide ko kaise dekhenge... Kitne me observe karna hai..... Please tell..
video me dikhaya hu...pehle 40X phir 100X
@@MLTLabManual alright...
Aapke pas itna knowledge hai , thats great..
Mujhe bhi sikhado😌
Me interviews de rahi hu aaj kal..
Where r u working?
100x pe
Super explain sir
You forget to give Cedarwood oil on glass slide.. After that you can view under microscope..
Very well explained
Thank You & Please share it in your WhatsApp contact
Aap kahan se ho
Hamko rbc or wbc Dikhta nahi hai mere pass Lishman Aaur Distil water hai Kya kami hai Bahut try kiye hai
Sir washing method galat hai Direct flow of water pe nhi finger pe water girne dete hai phir slide pe
Thank you sir ❤
Sir methenol se fix kiu karte hai smear ko
Acetone free methenol
Good experience
Sir आप पटना से है क्या
Madhubani
Nice sir
Very nice
वीडियो countinue डालिये
Sure! Dear
Sir what is difference between distill water and normal saline
Distilled water is the pure form of water which is made heating , boiling, cooling vapours, that is a hypotonic solution but saline made my adding sodium chloride in water which is isotonic solution use for IV fluids.
@@MLTLabManual thnx sir
Great explained thanks sirji🙏
Mithenal me Dena jaruri hai sir
Mandatary nhi h
Thank u sir ☺️
Sir , ya video SRM hospital ka h Na
Nhi ..mere lab ka h
@@MLTLabManual hlo sir dlc ki + kasie krna hai
Very helpful
Nice one***
Very very Thanks Sir
But you did not add the buffer!
Hme edta blood lena hota aa na ki finger prick
Sir ek request hai . Meri slide may black deposit bahut Jyada aate hai.. .agar dobara stain dal kr wash krti hu to platelets nahi dikhte... Plzzz help me Mai kya kru.... Hmari doctor roz bahut Jyada dantti hai Muje... Plz help me 😥😥
Black deposit aane ka matlab stain puri tarah spred nhi ho rha ..dilute proper kro ...ek baar se doctor se hi puch lo ki sir kaise Krna h ek baat bta do
Put the tissue paper on slide then pour the Leishman stain
Bhai methanol me kyun fix karte hai ? Bina uske direct stain kar sakte Hain?
Han kr skte hai ..lekin smear ko fix krne ke liye methanol me deep krte hai
Tlc count batyega bistsr se
Direct water nahi dalna hota hai stain slide par
Right
Nice video
Smear pe direct aani kon dalta
Thank you sir
Regular video daliye sir ji
Ok dear
TLC count kese kare
Thanks 🤗
Smear dry ayyinaka direct ga stain chesestharu kadha mari fixation enti process lo adhi ledhu kadha
Thanks sir
How to count the dlc smear
STAINING ROD TO KHARID LE ESR ROD PE LAGA RHA HE STAIN
Thank you
Eosin me to dip kiya hi nhi .... TLC ke liye Leishman & Eosin dono me dip karna hota hai.
not necessary
@@MLTLabManual where it is mentioned that it is not necessary to dip in eosin.
Oo bhai leishman stain contains eosin dye and methylene blue
GRA kay hai Sir
Nice sir g
Mstt
Tq sir🙏
Sir apni first drop discharge kyo nhi kyei
Not Mandatary to discard first drop
@@MLTLabManual yes its mandatory i think so...practical exams me external keen observation krte hey
Plz determine platelets DLC 🙏🙏🙏🙏🙏 Sir
Nice
Wrong air bubbles nehi hona chahiye
Don't squeeze the finger tip
Ek se 2 mint tak daal ke rakhte hai hum stain
But i can not do perfect smear. I also trying 😓😥. I am bmlt 3rd year student
Keep trying
Kitane film dekhegi..?
Blood squeeze kar ke nahi nikala jaata h
Blood smear are correct.
👍👍👍
Are mic 🎤to lelo aawaz hi nahin aa rahi
Ok