Leishman Stain Procedure and Blood Smear Preparation for DLC Count

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I’m Uday Chaudhary Welcome to MLTLab Manual
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leishman stain,leishman stain principle,leishman stain procedure,leishman staining of blood smear,leishman stain preparation,leishman stain in hindi,leishman stain composition,procedure of leishman stain,leishman staining method,how to prepare leishman stain,leishman stain kaise krte hain,leishman stain practical video,leishman stain kaise banaye,leishman staining,peripheral blood smear,blood film,differential leukocyte count,blood smear technique,dlc count,tlc count,romanowsky stain,blood smear staining procedure

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[@yakshinidevi3139]

Thank you so much for your effort

[@tanmayeenalavade394]

Please don't squeeze the finger while drawing capillary blood as the tissue fluid oozes out and mixes with the blood thus diluting the blood sample giving false results.

[@_ABHISHEKSAHA_MBBSO-]

Yaar lekin squeeze nahi karte to blood nikal ta hi nahi

[@tanmayeenalavade394]

@@_ABHISHEKSAHA_MBBSO- Uske liye needle 45 degrees angle par pakadkar 2 to 3 mm deep prick karo

[@_ABHISHEKSAHA_MBBSO-]

@@tanmayeenalavade394 thanks, I will try it in my next hemat practical

[@welcomeexam888]

Bilkul Correct bola apne

[@allifazhar]

We have to do DLC not TLC or TEC

[@Fardeenkhannnnnn]

Boht acche se bataya shukriya 🖤😃👏🏽

[@knowledgetv6497]

Thanks sir... This help me alot🙏

[@aijajkhan6660]

Very Helpful video 🙋🙋

[@ankitagayen970]

Thanks a lot 💓

[@kalyanikalyani8589]

Thank you so much sir

[@MdTOHIDUL-hl9wu]

thank you so much for this video

[@champadhali6746]

Thank you so much sir🙏

[@jagjitrandhawa800]

Thank you bro 👍

[@creazyboys4177]

This method is very egy 😊

[@Saru_0.0]

First i want to thank you a lot for this video, it helped me learn a lot!
And secondly I want to ask,
Why don't we put a cover slide on the specimen before putting it under the microscope?!
Hope you answer me soon!

[@drroguegaming2636]

On doing DLC there is no need of using cover slide. Hence its oil immersion 100x lens is used. The lens should touch the oil
After counting we have ti rub the tip of lens using spirited cotton

[@ourdream5440]

Nicely explain

[@knandini9403]

Nyc explanation 😊

[@AryaArya-lo7gm]

Nice presentation 🤩

[@999gy6]

Good explanation bro

[@aijajkhan6660]

Thanks so much sir 👍

[@asifboyz3933]

Very very Thanks Sir

[@labtechnicianlifedileepmee4892]

Super explain sir

[@rajendersinghthakur7465]

First drop of blood is discarded right ✅
Thank you sir

[@aartimaurya8495]

Thanku so much sir🤗

[@nirmalkotwal7534]

Good experience

[@mohammedismail2020]

Very helpful

[@muthyalanagesh7868]

Thanku so much sir

[@NEETmedicalGK]

Thanks.

[@lalitakanoje2386]

Thank you sir

[@nishagupta1351]

Thanks sir

[@suhailazmi7996]

Thank you very much sir ji

[@MLTLabManual]

My pleasure dear

[@technical1502]

Very nice

[@cspsharma1845]

Nice sir

[@Unkowmperson-lol]

Thanks 🥰

[@statusvideosa.s.18ok19]

Thanks 🤗

[@mvid1224]

What is the color of Leucocyte and malarea and trypanosmoma
When we use the lieshmin stain?

[@miraasif822]

Nice one***

[@umasamanta4090]

Thank you

[@MedicalBroadcast]

Very well explained

[@MLTLabManual]

Thank You & Please share it in your WhatsApp contact

[@shambhuyadav3456]

Nice video

[@sanasakkeer209]

Tnx dear

[@Swati2319]

Thanks 🙏🏻

[@hemabhardwaj847]

Sir we never take first drop of blood ....we have to wipe it

[@drroguegaming2636]

Yes true we have wipe the first drop

[@farukkhankhan4556]

Thanx sir

[@sangeetawairek6283]

Great explain//.👍🏻

[@MLTLabManual]

Thank you

[@subhashnaik4236]

Thank you for explaining

[@ashareelsrrr8089]

Thanku sir

[@saptarshisabui7223]

Thnx sir

[@rumarounta594]

Nice sir g

[@ss...4704]

Tq sir🙏

[@chainsinghrajput3590]

Thank u sir ☺️

[@humanityk2423]

Thanks sir from Bihar🙏

[@MLTLabManual]

My pleasure

[@yashitarawat5425]

@@MLTLabManual a

[@Mudrascience]

Good

[@kavyalal6735]

Nice

[@simmichaudhary2758]

👍👍👍

[@manmathshete6634]

Great explained thanks sirji🙏

[@pramilagupta433]

You can mix it by blowing also

[@shadablab5226]

No need of methanol fixation for Leshmin stain, its alcoholic stain

[@daliamahata9983]

Yes

[@Mrimposter2]

Right

[@sibaprasadmalla7556]

Right

[@thefootballanalyst4629]

Sir.. can I take a short part from this video to make a lecture for kerala higher secondary examination?

[@drroguegaming2636]

Nice ayit 🤣

[@riyamittal3712]

Just found your video... The best video.. Sir can you please explain how to use oil immersion.... Please😫🙏🙏

[@satishtandan8498]

high power me dekhne ke liye...

[@satishtandan8498]

normal lens se 100 times extra dikhta h oil me

[@aditiyadav5714]

Just take a drop of imersion oil on smear and watch it under a microscope 🔬 simple

[@misspujaoraon3276]

Lesmain dalne k 2 Min bad buffer solution 2-3 drop dalte h . Fir 5-10 min k bad use tap water se dhona h Uske bad tissue se sukha k use microscope k liye ready krte h

[@mangalikhanpathologist]

Kaha se ho

[@goraarora2456]

M. P. Stain reagent A and b hota hai sir red or blue konsa use pehle hota hai btaye

[@lab_druggist]

Plz determine platelets DLC 🙏🙏🙏🙏🙏 Sir

[@Vishal_Gupta_96]

Mstt

[@arunzaildaar9832]

We don't need to fix the slide in ethanol when we done Leishman stain

[@rawia8325]

I think that also

[@jitendramandal9916]

Hamko rbc or wbc Dikhta nahi hai mere pass Lishman Aaur Distil water hai Kya kami hai Bahut try kiye hai

[@rawia8325]

But you did not add the buffer!

[@badmaschora6641]

Regular video daliye sir ji

[@MLTLabManual]

Ok dear

[@bantupallisravani9110]

Smear dry ayyinaka direct ga stain chesestharu kadha mari fixation enti process lo adhi ledhu kadha

[@drsahiba215]

You forget to give Cedarwood oil on glass slide.. After that you can view under microscope..

[@hindipoem4770]

👍🏼👍🏼👍🏼👍🏼👍🏼

[@anuraglakra4690]

Sir washing method galat hai Direct flow of water pe nhi finger pe water girne dete hai phir slide pe
Thank you sir ❤

[@bubunamohanty956]

Thank sir

[@nyashalove2music]

We dont have to rinse it completely with distilled water na otherwise the slide will be washed up completely and we will get negative results so its better to put the thumb on the slide so that it shouldn't rinse completely otherwise it will show wrong results

[@MLTLabManual]

Thanks for your feedback

[@deepnag89]

Methanol kitna percent use karte hai sir

[@biplobmudoi9090]

Tomorrow my exam thank god 😅

[@MLTLabManual]

Best Wishes

[@ArshDeep-xo1jp]

Hme edta blood lena hota aa na ki finger prick

[@sahanazaktarmazumder8607]

Why you are not adding cedarwood oil

[@MLTLabManual]

No need

[@Kushkm]

वीडियो countinue डालिये

[@MLTLabManual]

Sure! Dear

[@studypoint9697]

Index finger he ku use krte h prick ke liye hmesha ...!!!

[@shrutishradha604]

How to prevent rouleaux formation ??

[@MLTLabManual]

Make thin smear to avoid Rouleuxe formation

[@chaithranayak9539]

How to count the dlc smear

[@pramilagupta433]

Don't squeeze the finger tip

[@brandguruvlogs8]

Patna में कोई Lab या teacher बताइये जो manual काम सिखाते हैं

[@MLTLabManual]

No Idea

[@FutureMotivatorAi]

Metanol sei fix bhi karni hoti hai slide

[@MLTLabManual]

Leishman me nhi krte hai, wo giemsa stain me krte hai

[@aditiyadav5714]

Sir hm smear 100x pe dekhte hai na 🙄

[@lalpathpsc5529]

STAINING ROD TO KHARID LE ESR ROD PE LAGA RHA HE STAIN

[@gurubrargurubrar3265]

Sir , ya video SRM hospital ka h Na

[@MLTLabManual]

Nhi ..mere lab ka h

[@sukhvirsinghkhalsa2751]

@@MLTLabManual hlo sir dlc ki + kasie krna hai

[@ravinakumari1919]

Distilled water kitne drop daalna hai wo bhi bataaea

[@MLTLabManual]

Leishman stain ke double volume

[@pravinkushwaha856]

Sir what is difference between distill water and normal saline

[@MLTLabManual]

Distilled water is the pure form of water which is made heating , boiling, cooling vapours, that is a hypotonic solution but saline made my adding sodium chloride in water which is isotonic solution use for IV fluids.

[@pravinkushwaha856]

@@MLTLabManual thnx sir

[@medicohina8186]

Direct water nahi dalna hota hai stain slide par

[@pritysona3954]

Right

[@sunandade7552]

But i can not do perfect smear. I also trying 😓😥. I am bmlt 3rd year student

[@MLTLabManual]

Keep trying

[@ayanamahira4131]

Distilled water se ku tap water use kr sakte hai

[@drroguegaming2636]

Yes

[@mltgourav2753]

Yes it can be with tap water

[@ashokdasmovies9620]

TLC count kese kare

[@sangeetawairek6283]

Ek bar ye bataiye ki slide ko kaise dekhenge... Kitne me observe karna hai..... Please tell..

[@MLTLabManual]

video me dikhaya hu...pehle 40X phir 100X

[@sangeetawairek6283]

@@MLTLabManual alright...
Aapke pas itna knowledge hai , thats great..
Mujhe bhi sikhado😌
Me interviews de rahi hu aaj kal..
Where r u working?

[@aditiyadav5714]

100x pe

[@Ali-eh9ry]

Bhai methanol me kyun fix karte hai ? Bina uske direct stain kar sakte Hain?

[@MLTLabManual]

Han kr skte hai ..lekin smear ko fix krne ke liye methanol me deep krte hai

[@AtleeKumar-hq6xp]

Hai yarrrr krta to haii😂😂
Maanta h ki nhi

[@puleshsahu1236]

GRA kay hai Sir

[@ajaysharma4247]

Eosin me to dip kiya hi nhi .... TLC ke liye Leishman & Eosin dono me dip karna hota hai.

[@MLTLabManual]

not necessary

[@ajaysharma4247]

@@MLTLabManual where it is mentioned that it is not necessary to dip in eosin.

[@arunzaildaar9832]

@@ajaysharma4247 bhai mere Leishman stain me single stain use hoti h eosin khi bhi use ni hoti

[@drroguegaming2636]

Oo bhai leishman stain contains eosin dye and methylene blue

[@bishopmalik8519]

Smear pe direct aani kon dalta

[@jitendramandal9916]

Mithenal me Dena jaruri hai sir

[@MLTLabManual]

Mandatary nhi h

[@parshurammahto7994]

Aap kahan se ho

[@Sadir4483]

Blood smear are correct.