The wavelength of acceptor emission doesn’t change at any point - it is indeed independent of absorption energy. However, in a FRET system relying on ligand binding to bring donor and acceptor into range for interaction, it is the ligand binding that stimulates emission changing from basal to excited level. Therefore the wavelength doesn’t change, but the signal level does.
Wouldn't a longer wave length lead to LOWER energy??
why does acceptor emission change upon binding of the ligand? I thought the emission spectrum is independent of the absorption energy.
The wavelength of acceptor emission doesn’t change at any point - it is indeed independent of absorption energy. However, in a FRET system relying on ligand binding to bring donor and acceptor into range for interaction, it is the ligand binding that stimulates emission changing from basal to excited level. Therefore the wavelength doesn’t change, but the signal level does.
Wouldn't a longer wave length lead to LOWER energy??
Stewart Brands hc/lambda ?? gets larger when lambda gets smaller.
Your right, I think 05:38 is wrong, a slip of the tongue I think