Microscopy: Fluorescent Protein Indicators (Roger Tsien)

Learn more: www.ibiology.org/talks/fluore...
This talk discusses how fluorescent proteins have been turned into indicators for a wide variety of biological molecules, including pH, ions, redox potential, and signaling molecules like phosphoinositides. It also covers reporters used to measure the activity of enzymes like kinases, phosphatases, and proteases. It covers both single proteins whose intensity or wavelength change, as well as reporters using resonance energy transfer (FRET).

[@JinoLim]

Clear and in-depth explanations.
Everything you need to know about Fluorescent microscopy in one video.
It's hard to find good resources. Thank you!

[@JH-it2yr]

Very detailed and clear! No ads interrupting! Thank you very much professor!

[@harambeismydad8440]

Dear Roger,I love you,Kai Lok Man

[@tompike7239]

Truely amazing lecture, thank you!

[@tompike7239]

what a wonderful man the world has lost.

[@MrTridac]

Reverse engineering life.