@@earlinebresil5751 Yeah I stopped at 15 seconds in cause I HATE asmr and trash like that. I'm sure he has good things to say but no way am I watching this
If you can't estimate it by yourself with your data set (which shouldn't be that difficult to begin with), then you could use a Lineweaver-Burk plot (or another linearisation) and calculate Km and vmax off of that.
Hello, thanks for the video it was very helpfull, my question is i have a very low [s] and Vmax and excell didnot want to give me the Km value. i have tried the same values as the video and i get the exact answer but when i try my own data i dont get any thing at all. (my data is as low as to the power of -06) may be excell doesnt work on such low values? (am using mM as a unit for S and umol/min for Vmax).
There are some really great tutorials on editing out the mouth smacking noises on your video. If you search for "how do I stop disgusting saliva sounds when recording," you should find a bunch.
This video literally saved my Biochemistry II lab report about enzyme kinetics. Thank you very much and keep it up with the good videos!
same here from across the pond haha
So much mouth moistening
I can't finish the video. It's grossing me out.
its like ASMR mmmm
Omg I thought it was just me😂😂😇
@@earlinebresil5751 Yeah I stopped at 15 seconds in cause I HATE asmr and trash like that. I'm sure he has good things to say but no way am I watching this
You sound like the narrator for Winnie the Poo! Thank you for this helpful video
No, the max velocity is 10 in your case. But Km kicks in at half of the maximum velocity of enzyme activity (5).
Why "10" for Km and what is the equation you put in the field. Pls explain
Thank you for the video! I really appreciated it!
You sound like Dr. Walter Lewis which is cool.
Thank you for the tutorial! How did you draw the inserts at the half points in the graphs?
Hey but how did you estimate the Km and Vmax
If you can't estimate it by yourself with your data set (which shouldn't be that difficult to begin with), then you could use a Lineweaver-Burk plot (or another linearisation) and calculate Km and vmax off of that.
Very good explanation, thanks a lot! :)
Thank you so much 🙌
Hello, thanks for the video it was very helpfull, my question is i have a very low [s] and Vmax and excell didnot want to give me the Km value. i have tried the same values as the video and i get the exact answer but when i try my own data i dont get any thing at all. (my data is as low as to the power of -06) may be excell doesnt work on such low values? (am using mM as a unit for S and umol/min for Vmax).
Thank you so much, for the tutorial, you helped me a lot
How would you include the errors associated to each point of the graph? What about the Km and Vmax?
Helped me a lot, thanks!
ASMR worthy
Thank you. This helped a lot.
After the solver, all of my calculated V and Km became 0. I don't know what to do :(
You need to set your solver to look for the minumum and not the maximum.
have a concentration but nothing else and need to determine V
how do you consider the half values?
Thank you!
I cant with the mouth moistening. Thanks tho.
thx alot very helpful !
First thought: David Attenborough XD
anyone whos watched this pls summarise it for me because i cannot do it, listen to this man smack his mouth full of spit:/
I cant listen to this, too much mouth noise.
Why the hell not get a decent microphone if you're planning on making TH-cam videos?
There are some really great tutorials on editing out the mouth smacking noises on your video. If you search for "how do I stop disgusting saliva sounds when recording," you should find a bunch.
extremely unhelpful