hi sir i have one doubt. which is the correct phase for find the magnetic force microscopy image. because i could not find out the correct image in mfm .i hope you definitely clear my question.
Hello Sir, I have recently been trying to function the AFM and I have a question regarding the tuning. Why do we change the target tapping signal to 5V rather than just leaving it?
Dear Dave, I have really enjoyed the tutorial about Tapping Mode AFM. I would like to know if is there a similar tutorial about kelvin probe force Microscopy (KPFM). Mainly in lift TappingMode KPFM. Thank you in advance.
Hey, This is Ala Eftaiha, a professor of physical chemistry at the Hashemite University, in Jordan. I have a “veeco di-innova” AFM and I have a problem in aligning the laser on the cantilever. First, the cantilever was placed above a stainless steel mounting disk, where the probe was centered in the Navigator window. After that, I started to use the laser adjustment knobs on the right hand side of the SPM head to direct the red laser beam onto the back of the cantilever, but unfortunately, I cannot find the laser spot at all. Actually, I doubt if the laser works or not. To check that, I have replaced the stainless steel mounting disk with a piece of white paper, which I expect to give a better laser contrast, but I cannot identify laser spot. The thing that make me confused is that after I unplug the head, the laser diode still on! I am wondering if there is any suggestion to solve this problem. Sincerely, Ala’ Eftaiha
Have you tried putting the laser on the frame that holds the cantilever? Sometimes I place it there, then walk the laser to the diving board (cantilever) - often I don't use the microscope, I just watch the laser spot until its in the right place. Another method is getting your tip close to the sample (< 1 mm), then you can see the laser on the surface, although be aware that the laser angle is skewed so it'll appear to the right of the cantilever when it's in the proper place. Another issue that sometimes comes up in the microscope is if the illumination is too high it'll make the laser dot very faint - try turning the lighting low or even off to help find the laser. Hope this helps.
That is a calibration grating - engineered so that the spacing of holes (called the pitch) is repeated at specific increments, say 1 micron, for example. These gratings are scanned so that you can check the x & y distance in your scans, and adjust the scale of the software if necessary.
Hello Dave, This is Nayan from India. Thank you very much for the basic demo. I have two questions, and hope you could help me out with those. 1. Below the setpoint data, there are options for drive and phase. What are the basic utilities of the same. Can we play with them too, how? 2. I would appreciate your valuable tips on small area scanner as well. How, when and what purpose we could use small area scanner.
Certainly Nayan. 1) drive and phase have to do with the vibrations of the cantilever when it's in tapping mode - as we use the program to tune these settings, I do not recommend changing them here. If the vibration of your cantilever is off, it is better to re-run the tuning program covered in the video. 2) This depends largely on the size of the features you wish to image and study. If I wish to look at some features close to a micron in size, spaced a micron or two apart, then a 10-20 micron image may be best, to best represent the surface morphology. If, on the other hand, there are features 50 nm in size, spaced 100 nm apart, then a 2-3 micron image may be best. The large area scanner is best used for 3-100 micron-sized images, while small area scanners are better for sizes below 3 microns. As most of what I study is best shown at 3 microns or more, I often stick with the large area scanner, but for some really flat surfaces where the height may only vary by a monolayer or two, I switch to the small area scanner and do a 1x1 micron image. Hope this helps.
hi sir i have one doubt. which is the correct phase for find the magnetic force microscopy image. because i could not find out the correct image in mfm .i hope you definitely clear my question.
Hello Sir,
I have recently been trying to function the AFM and I have a question regarding the tuning. Why do we change the target tapping signal to 5V rather than just leaving it?
Dear Dave,
I have really enjoyed the tutorial about Tapping Mode AFM. I would like to know if is there a similar tutorial about kelvin probe force Microscopy (KPFM). Mainly in lift TappingMode KPFM.
Thank you in advance.
Hey,
This is Ala Eftaiha, a professor of physical chemistry at the Hashemite University, in Jordan.
I have a “veeco di-innova” AFM and I have a problem in aligning the laser on the cantilever.
First, the cantilever was placed above a stainless steel mounting disk, where the probe was centered in the Navigator window. After that, I started to use the laser adjustment knobs on the right hand side of the SPM head to direct the red laser beam onto the back of the cantilever, but unfortunately, I cannot find the laser spot at all.
Actually, I doubt if the laser works or not. To check that, I have replaced the stainless steel mounting disk with a piece of white paper, which I expect to give a better laser contrast, but I cannot identify laser spot.
The thing that make me confused is that after I unplug the head, the laser diode still on!
I am wondering if there is any suggestion to solve this problem.
Sincerely,
Ala’ Eftaiha
Have you tried putting the laser on the frame that holds the cantilever? Sometimes I place it there, then walk the laser to the diving board (cantilever) - often I don't use the microscope, I just watch the laser spot until its in the right place.
Another method is getting your tip close to the sample (< 1 mm), then you can see the laser on the surface, although be aware that the laser angle is skewed so it'll appear to the right of the cantilever when it's in the proper place.
Another issue that sometimes comes up in the microscope is if the illumination is too high it'll make the laser dot very faint - try turning the lighting low or even off to help find the laser.
Hope this helps.
Dear Dave,, Would you help me, inform about of the cos and sin in use AFM ?
Hello Sir
Can any one tell me what is this flat surface you scanned with AFM and you showed in the video ?
Thanks
That is a calibration grating - engineered so that the spacing of holes (called the pitch) is repeated at specific increments, say 1 micron, for example. These gratings are scanned so that you can check the x & y distance in your scans, and adjust the scale of the software if necessary.
Hello Dave,
This is Nayan from India. Thank you very much for the basic demo. I have two questions, and hope you could help me out with those.
1. Below the setpoint data, there are options for drive and phase. What are the basic utilities of the same. Can we play with them too, how?
2. I would appreciate your valuable tips on small area scanner as well. How, when and what purpose we could use small area scanner.
Certainly Nayan.
1) drive and phase have to do with the vibrations of the cantilever when it's in tapping mode - as we use the program to tune these settings, I do not recommend changing them here. If the vibration of your cantilever is off, it is better to re-run the tuning program covered in the video.
2) This depends largely on the size of the features you wish to image and study. If I wish to look at some features close to a micron in size, spaced a micron or two apart, then a 10-20 micron image may be best, to best represent the surface morphology.
If, on the other hand, there are features 50 nm in size, spaced 100 nm apart, then a 2-3 micron image may be best. The large area scanner is best used for 3-100 micron-sized images, while small area scanners are better for sizes below 3 microns. As most of what I study is best shown at 3 microns or more, I often stick with the large area scanner, but for some really flat surfaces where the height may only vary by a monolayer or two, I switch to the small area scanner and do a 1x1 micron image.
Hope this helps.