Unbelievable! I was working in a hood subbing orchids in 1983. Then to big scaled up industrial size propagation for export, then labs, and genetic modifcation shooting genes into callus with a modified gun. Then virus elimination for quarantine germplasm. Yet after all this I click on this little old vid and I still learn something new. Never seen a roasting bag used for sterile transfer. Never knew it was sterile. thanks for this upload. It's a pearl.
I really enjoyed this video. I'm starting to experiment with tissue culture but I know it's pretty tough to get it going the right way, using different growing media's and nutrient hormones etc.. This is so interesting and I just love the science behind all different walks of nature. Thank you for sharing! I wish I could visit a lab that does this. So fascinating.
The hood is going to make your life way easier. I use a rubber made container turned on its side at home. My contamination in the rubber made container is about 30 percent. At work I use a flow hood and my contamination is 5 to 10 percent.
Hallo,i want to do this at home,what you use for making de voeding of the plant,i have a lot of flowers to try.can you give me the first step for beginning
I tried getting the chemicals from Sigma-Aldrich but they won't sell to individuals or home users. So ended going to eBay and Phytotech labs. Also, as he states you can use normal table sugar for sucrose.
@@joekunedo ahh I haven't had opportunity to perform tissue culture yet as I've been waiting for my orchid seed to mature. Orchids require similar procedures, will be able to test in about a month i think.
Yup, that's the first hint to tell this is just a demo. Once they pulled it out of the bag to see it better and show the cameras it was confirmed. Good point though: clean, tight/well fitting clothing is an overlooked lab standard.
And as I've been in the industry of broad scale mass production with a dozen staff chopping away, it was always, hairy arms and beards that produced the most contamination. Even with hexafoam, body hair just carries too many bugs.
![แฉกลโกงงานวัด 2024 [ โกงมั้ยครับ ep.106 ] | DOM](http://i.ytimg.com/vi/taC5dpP3HXA/mqdefault.jpg)
Unbelievable! I was working in a hood subbing orchids in 1983. Then to big scaled up industrial size propagation for export, then labs, and genetic modifcation shooting genes into callus with a modified gun. Then virus elimination for quarantine germplasm.
Yet after all this I click on this little old vid and I still learn something new.
Never seen a roasting bag used for sterile transfer. Never knew it was sterile.
thanks for this upload. It's a pearl.
What you learned to get in these field of jobs?
I really enjoyed this video. I'm starting to experiment with tissue culture but I know it's pretty tough to get it going the right way, using different growing media's and nutrient hormones etc.. This is so interesting and I just love the science behind all different walks of nature. Thank you for sharing! I wish I could visit a lab that does this. So fascinating.
Such a helpful video (even though it was apparently filmed before I was born). I followed your steps in my latest vid :)
Damn it. I just spent $500 on a small laminar flow hood and I could have done all this in a 20 cent bag..... sigh.....
The hood is going to make your life way easier. I use a rubber made container turned on its side at home. My contamination in the rubber made container is about 30 percent. At work I use a flow hood and my contamination is 5 to 10 percent.
Where can you buy that hood for $500?
@@destinee9903 At the time it was on eBay but I can't any at that price anymore
Could sucrose be replaced by sugar from market ?
Yes, they said table sugar works. Just make sure you don't accidentally use artificial sweetener or syrups. Most of those won't work well or at all.
What temps are you storing tissue once inoculated? Incubator or refrigerator? Any gas overlays?
Could you show, how do you prepare MS whit hormone for african violet? Which kinds of hormon can ı prefer and how much ml?
Hallo,i want to do this at home,what you use for making de voeding of the plant,i have a lot of flowers to try.can you give me the first step for beginning
Where can I get information on large scale tissue culture and freezing plants cells
Thank you
What is that high pitched tone in the background?
plant screams
you see kids back in the day we had VHS.....
At what temperature should the test tubes be stored?
26 degrees celcius
You don't have a laminar flow hood? I've got one of those at my house...
Nerd.
@@russellwilliams7589 Thanks :) You caught me.
found the mycologist
This looks like a high-school in 1995 dude give him a break
@@cameronm896 I had to look up what a mycologist did. Thank you, I learned something today.
Where's cartman?
was this filmed in 1998?
1994
Would be a great 'scientist' character in a movie.
Hi.. where can we buy this materials ang chemical?
I tried getting the chemicals from Sigma-Aldrich but they won't sell to individuals or home users. So ended going to eBay and Phytotech labs.
Also, as he states you can use normal table sugar for sucrose.
@@fortuneflux how did it go?
@@joekunedo ahh I haven't had opportunity to perform tissue culture yet as I've been waiting for my orchid seed to mature. Orchids require similar procedures, will be able to test in about a month i think.
Devonly to break the dormancy period, you should try soaking your plant/seed in a solution of gibberellin.
@@fortuneflux I worked a year in an orchid nursery with a lab for flasked stock. We used banana and potato as the main food in the agar. Worked well.
Can this be done with dried leaves ?
no.
what he means is "no, because dried leaves are dead tissue. You tissue that is alive and the fresher the better"
Nice 👌
💗🥰💗
Please don't look in the camera sir. I'd like to keep my soul.
what about his filthy tie dragging all over everything?
Yup, that's the first hint to tell this is just a demo. Once they pulled it out of the bag to see it better and show the cameras it was confirmed. Good point though: clean, tight/well fitting clothing is an overlooked lab standard.
And as I've been in the industry of broad scale mass production with a dozen staff chopping away, it was always, hairy arms and beards that produced the most contamination. Even with hexafoam, body hair just carries too many bugs.