How to use Spread Plate technique

Warm wishes from ‪@MicrobiologyMantra‬ .
This video explains about the spread plate technique which is one of the isolation methods and is very important. I hope this video provides all information you need. Please comment if you have any question.
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Script from video:
Spread plate technique is one of the isolation methods. This technique is used to isolate and count microbial colonies from a liquid sample.
This technique is very useful in the microbial testing of food, and other environmental samples like soil and water.
If the sample that need to be tested is not in the liquid state, then it needs to be grinded, dissolved in suitable liquid, and then used for testing.
To perform this technique, we need test tubes, pipettes, glass spreader which is in L shape, Nutrient agar plates, Bunsen burner, and alcohol or acetone.
Let’s see how this is done.
Take out 0.1 millilitre of sample using a sterile pipette, and place it in the centre of the agar plate.
Here we forgot to mention one important step.
Before adding the sample to the plate, we have to prepare serial dilutions of the sample in order to bring the microbial cells to the countable number. We will discuss more about serial dilutions in our next video.
Now that we are ready with the sample dilutions, it is very important to label each plate with the dilution factor.
Ok. Now, after adding the sample to the plate, take the glass spreader, dip in to the alcohol or acetone, and expose to the flame in order to make it sterile.
Wait for a few seconds, and then spread the sample evenly all over the agar surface.
This can be achieved by gently rotating the plate, and holding the spreader at 45 degree angle. This enables us to operate the spreader smoothly and decreases the hand pressure on the agar surface.
We can also make use of a rotator to rotate the plate with more ease.
Ensure that the sample is distributed all over the agar surface.
Then incubate the plates at 37 degree celsius for 24 hours.
After the incubation period, you should see isolated and countable colonies distributed on the agar surface in one of dilutions.
With this count, we can calculate the population of microbes present in the original sample.

[@kinjallikhiya1274]

Thank you so much bro😊 , tomorrow is my practical exam 😂

[@abdulrahuman2473]

Good

[@swethamicro5420]

Easy to understand very useful video

[@tekukunoka2384]

Thank you so much for sharing this video sir, I have my practicals semester tomorrow which is basically going to be this morning actually anyways, this method which you explained in this video is easy to understand 😊

[@soniyakanmani8484]

Easy definitions superb sir

[@itsfahimhere]

Thank you sir, I can understand 👍

[@uniquecookingofmymother4447]

Useful video

[@oluIsaac7]

Good sir ❤❤

[@dohawahedy]

❤❤

[@lilianas9702]

Hi, I have a question about drying the media before inoculation? Drying is generally at 37oC / 1h.

[@nutannimagde3650]

Thanks sir😊

[@amanrajjab6260]

Is video ka 2nd part konsa hy mujhy mil nahi raha ap k channel pr

[@sylwiazacharko1222]

Great video, I have a microbiology little assigment (part of my Access to HE) and one of the question asks about advantages and disadvantages of 'spread plate solid-to-solid- and i am going bananas, as everywhere I am googling is 1st to dilute the solution and the transfer it... which means it would be from liquid to solid? any help please?

[@instantnotesinmicrobiology3100]

👍

[@vejushiva8291]

Thank you bro ❤️ tomorrow I have spot exam 😉😉

[@akhilava2080]

How to keep for incubation.....inverted?

[@amanjindal5466]

Sir hum serial dilution ki Karte hai??

[@amanrajjab6260]

A.O.A

[@uniquecookingofmymother4447]

Background music is bad..download good music