Really helped me understand this concept better for my lab class. My instructor sent me a video which was very difficult to understand, but this was much more comprehensible. Thanks
A really good and detailed video. I am just wondering if i need a large amounts of samples how do i go about. Like if my sample introduction system requires atleast 10m of a sample.
Great video! I think the best so far I have watched. But I would like to ask a question: Is it advisable to use MeOH throughout if acetonitrile is not available?
First of all, thank you very much for the tutorial. It is really useful. I wanted to ask if the conditioning of the direct phase columns is the same. Thanks so much again!
the column of C18 is only a cm or 2 in length. far shorter than the normal phase columns i've seen on vid's here on u-t. could you comment on this feature of C18 columns , please
Im a Forensic chemistry student and if never done a Solid Phase Extraction. tho it does look very similar to a Resin Exchange Chromatography experiment that i've done but fortunatly for the sake of time the experimenter could control flow
While you don't have to, it is an option to dry the cartridge before eluting the samples. By taking this step it helps further disrupt the stationary phase/analyte interaction to promote elution.
Ashmeeta Chera an Indian for styling purpose she's just pronouncing it as Ashee Shera😂 for sounding like "Britishers" very bad and shameful it is! That you are shy to take your own name respect 🇮🇳 your culture and language (mother tongue) @the woman in the video
I've read it one million times in my theory book of analysis and with a 8 min video I understood it better than ever
Seriously this is the best video on yt as of now for spe
Really helped me understand this concept better for my lab class. My instructor sent me a video which was very difficult to understand, but this was much more comprehensible. Thanks
Awesome video!!! thx a lot!
Really awesome job guys! Thank you!
Thank you this help out to undestand better SPE . Really nice moderators
Thank you, very clear demonstration.
Also, it was nice to have Ashmeeta there to represent the human species! 😀
love the explanation thank you!
A really good and detailed video. I am just wondering if i need a large amounts of samples how do i go about. Like if my sample introduction system requires atleast 10m of a sample.
Great video! I think the best so far I have watched. But I would like to ask a question: Is it advisable to use MeOH throughout if acetonitrile is not available?
First of all, thank you very much for the tutorial. It is really useful.
I wanted to ask if the conditioning of the direct phase columns is the same.
Thanks so much again!
Nice work!
Nice!!!
Hey, can we perform this for extraction of Isoquercitrin from petals of Dianthus.pavonius?
the column of C18 is only a cm or 2 in length. far shorter than the normal phase columns
i've seen on vid's here on u-t. could you comment on this feature of C18 columns , please
Thank you, iam an undegraduated student, and i use this methode to separated my peptide sample
We are glad if it was helpful! Please let us know if there are any other demonstrations you would like to see in the lab.
Thanks!
Hi is it possible to use this method for the determination of concentration of caffeine
I want this manual Solid phase extraction kit I.e. Spe cartridge and push rod. Can you provide me the complete set up at least cost
Im a Forensic chemistry student and if never done a Solid Phase Extraction. tho it does look very similar to a Resin Exchange Chromatography experiment that i've done but fortunatly for the sake of time the experimenter could control flow
Before eluting the samples, do we have to dry the cartridge?
While you don't have to, it is an option to dry the cartridge before eluting the samples. By taking this step it helps further disrupt the stationary phase/analyte interaction to promote elution.
What solvents are the dyes originally in?
Hi! Great question, for this demonstration we used an aqueous based dye solution.
@@Phenomenex thanks
@@Phenomenex Is that why you passed water through the column before you injected the samples?
the chatter ends at 3:38 ... then the experiment begins
im sure its totally fine, and im just weird, but the micropipette sitting horizontally with the tip on drove me a little crazy
Hey Josh, we are so sorry about that! Which part of the video are you referring to? Because I am sure that would make us a little crazy too.
@@Phenomenex 3:41
4:03 to almost 5:00
and again at 5 02
why is your micropipette HORIZONTAL IN 4:18 noooOOOOO
it only matters if there is fluid in the tip
Ashmeeta Chera an Indian for styling purpose she's just pronouncing it as Ashee Shera😂 for sounding like "Britishers" very bad and shameful it is! That you are shy to take your own name respect 🇮🇳 your culture and language (mother tongue) @the woman in the video
THIS is solid phase extraction? It's just a typical column chromatography for god's sake. Why is it not just called column chromatography?