I've just bought the microscope and immersion oil is something I'd never come across before. Thank you for this very clear and concise explanation. I think you may well have saved me money and grief!
Thanks very much. I bought a Japanese (Tokyo) microscope in the Recycle Shop for a wapping 38 euro. It even includes the quality control checklist. And guess what, it has a 100x objective, that my secondary school never had. This video is great. When you know microscope basics, you can carefully do the first oil immersion viewing. And the warning, never get any oil on the other objectives, for you really need to buy a new one, that is a good tip. I'll make it a habit to remove the object glass that has immoil, immediately. Keep the other objectives away. Hai!
I'm learning how to use a microscope that I got to help with some projects I'm working on. I'm not a professional or student, so I wasn't taught any of this. I'd actually never even heard of using oil like this before I got my scope. I'm SO glad I watched this before using oil! (It's probably in the instruction manual, but who reads those?)
Do you know what RTFM means? Read that funny manual! There's often things you MUST know, because you'd NEVER have guessed it. RTFM is a very old computer nerds joke.
Are their lower power oil sealed objectives available to avoid situations where students or trainees would likely make this kind of handling mistakes ? Great video.
Hi Erik. Good question! There are sealed 40X objectives out there, but nothing below that, unfortunately. In most cases the 40X and 10X objectives cannot even reach the stage. If they get oil on them, it is often due to poor cleaning practices.
We teach NOT to lower the stage to add the oil because you should only use the fine focus with the oil immersion and never the coarse focus. Is that not standard practice?
There are people that break object glasses, and there are people who see what they are doing. On some microscopes there is no fine focus, then you can just as well lower the stage. And be careful. Correct me if I'm wrong.
Good question! The bottom of the 40X objectives are usually sealed somewhat, but if oil is left on the lens, it can slowly wick its way past and around that protective barrier.
I've just bought Optika B-293PLi model microscope which has 100x Oil/Water immersion objective. I didn't use cover slip while looking water because of that, but when I changed 100x to 40x dry objective, 40x also touched water a couple of times. At first, I didn't pay attention to the situation, but now, I'm worried that I could damage 40x objective lenses irreversibly. Could 40x objective's lenses be damaged with these few occurrences?
Curious Questions I would not worry about the 40x being damaged by dragging through water or immersion oil a few times - should not cause any problem...use lens tissue and lens cleaner to gently clean the 40x lens - but should be good to go - the lens is sealed with cements that the water will not penetrate and water will not damage the lens or lens coatings
Typically you start with the lowest magnification and work your way up. This is so you can find the area of interest and get closer to it. Here's a link to our video on how to use a clinical microscope from start to finish. th-cam.com/video/cnj2y_Bpu-w/w-d-xo.html I hope that helps!
@@lgsmicroscopes4328 found information, you do oil the oil condenser for 100x oil, but not with the none oil objectives. Its is an oil condenser. Hope this help you.
In compound microscopes, the terms like "45X" and "10X" usually refer to the magnification levels of the objective lenses. These numbers indicate how much the image is magnified when observed through the microscope. However, these objective lenses are not typically designed for oil immersion. Oil immersion lenses are designed differently and have specific characteristics that make them suitable for use with immersion oil. Oil immersion objective lenses are used for higher magnification, typically 100X or higher. They are specially designed to work with immersion oil, which has a refractive index similar to that of glass. This oil is placed between the front lens of the objective and the glass slide, improving the numerical aperture and resolution of the microscope. This technique is particularly useful when observing very small and detailed structures, such as bacteria or fine cellular components. Lower magnification objective lenses, like 10X and 45X, are not designed for oil immersion because they are not intended for the same level of high-resolution work that requires oil immersion. Additionally, using immersion oil with lower magnification objectives might result in distorted images, reduced image quality, and even damage to the lens. So, while objective lenses are available in various magnifications, the use of oil immersion is specifically limited to higher magnification objectives that are designed for this purpose.
Short, sweet and to the point. Good clarity.
I've just bought the microscope and immersion oil is something I'd never come across before. Thank you for this very clear and concise explanation. I think you may well have saved me money and grief!
Same story. I found a secondhand one and I can't wait till I get it back, a friend needed it.
short and full of the neccessary information, exactly what im lookin for. Thanks!!!!
thank you so much. I am currently taking microbiology from home and this video has been great.
Thanks very much. I bought a Japanese (Tokyo) microscope in the Recycle Shop for a wapping 38 euro. It even includes the quality control checklist. And guess what, it has a 100x objective, that my secondary school never had. This video is great. When you know microscope basics, you can carefully do the first oil immersion viewing.
And the warning, never get any oil on the other objectives, for you really need to buy a new one, that is a good tip. I'll make it a habit to remove the object glass that has immoil, immediately. Keep the other objectives away. Hai!
Great and informative video! I will share this in class with my students. Thanks so much for this!
I'm learning how to use a microscope that I got to help with some projects I'm working on. I'm not a professional or student, so I wasn't taught any of this. I'd actually never even heard of using oil like this before I got my scope. I'm SO glad I watched this before using oil! (It's probably in the instruction manual, but who reads those?)
Do you know what RTFM means? Read that funny manual! There's often things you MUST know, because you'd NEVER have guessed it.
RTFM is a very old computer nerds joke.
@@voornaam3191 I actually did dig up the manual and read it. There was nothing about immersion oil.
Too darn clear and to the point video 🤭🤭🤭 love it...
Are their lower power oil sealed objectives available to avoid situations where students or trainees would likely make this kind of handling mistakes ?
Great video.
Hi Erik. Good question! There are sealed 40X objectives out there, but nothing below that, unfortunately. In most cases the 40X and 10X objectives cannot even reach the stage. If they get oil on them, it is often due to poor cleaning practices.
I think it's just better to get a 60x dry objective. I see that 100x oil isn't as amazing as it sounds
Thank you! That was precisely what I needed.
We teach NOT to lower the stage to add the oil because you should only use the fine focus with the oil immersion and never the coarse focus. Is that not standard practice?
I 100% agree! I was shocked to see this in a demo video. 😳
My microscope didn't have a fine focus and have a 1000x oil immersion
Good to know...Thanks.
There are people that break object glasses, and there are people who see what they are doing. On some microscopes there is no fine focus, then you can just as well lower the stage. And be careful. Correct me if I'm wrong.
Thank you. You saved me from my newbie ignorance. 🙂
Thanks! I've been wanting to look at some of my bdelloid rotifers at very high magnification!
Newbie here. Thanks for doing this video. Would have probably ruined my 40x had I not seen this.
Thank you. This was a great lesson.
Thanks boss. I finally took out my nikon microscope and have started exploring it. This helps as I don’t know the purpose of some parts.
Thanks
Great video and great information
Cheers! Nice one.
Crazy question: Why not make a sealed 40x? Seems like you could charge a premium for them.
Good question! The bottom of the 40X objectives are usually sealed somewhat, but if oil is left on the lens, it can slowly wick its way past and around that protective barrier.
At least they finally made a 100x dry that doesn't benefit from oil immersion but it is an outrageous unaffordable price.
Thank you. Superb information.
Very well explained thanks.
One should never try to clean the lower objectives?
Thanks for sharing. God bless you
Perfect., Thanks.
I've just bought Optika B-293PLi model microscope which has 100x Oil/Water immersion objective.
I didn't use cover slip while looking water because of that, but when I changed 100x to 40x dry objective, 40x also touched water a couple of times. At first, I didn't pay attention to the situation, but now, I'm worried that I could damage 40x objective lenses irreversibly.
Could 40x objective's lenses be damaged with these few occurrences?
Curious Questions I would not worry about the 40x being damaged by dragging through water or immersion oil a few times - should not cause any problem...use lens tissue and lens cleaner to gently clean the 40x lens - but should be good to go - the lens is sealed with cements that the water will not penetrate and water will not damage the lens or lens coatings
There’s a phone number at the end of the video, if you want a second opinion.
Thanksgiving you so much sir
when using light microscope do you start the largest or smallest distance between the lens and specimen/why?
Typically you start with the lowest magnification and work your way up. This is so you can find the area of interest and get closer to it. Here's a link to our video on how to use a clinical microscope from start to finish. th-cam.com/video/cnj2y_Bpu-w/w-d-xo.html
I hope that helps!
How to do it with the inverted microscope ?
شكرا جزيلا
Thank you! This helped
nice music man, blew my ears away.
That Microscope is not parfocal as you do not bring down the stage when going from the 40 to 100mm.
Thankyou 😊
Thank you!!!
thanks bro ❤️
Thanks
Do I have to use oil on my oil condenser with my none oil objective
No, you should never have to put oil on the condenser. Oil is only used on the 100X objective.
Hope that helps!
@@lgsmicroscopes4328 its a oil condenser
Darkfield
@@lgsmicroscopes4328 found information, you do oil the oil condenser for 100x oil, but not with the none oil objectives.
Its is an oil condenser.
Hope this help you.
really thanks
Thank you i feel stupid now
Um my objectives are forward facing so how do I do this?
Hi! It's the same process. You would just have to come in from a different angle to get the immersion oil onto the lens :-)
I want only this stand only I have already microscope
Impressive .........😇
are you still in business?
Thanks man 😋😋
Wow...
Ow, the word for lens in Polish is obiektyw, didn't know it was similar in English
It's an optical physics term
وين ترجمه ياجماعه
في ناس دخلت فيديو وهي عربيه بتعملي لايك
v nice
why the heck don't they just seal all of the objectives??????????
In compound microscopes, the terms like "45X" and "10X" usually refer to the magnification levels of the objective lenses. These numbers indicate how much the image is magnified when observed through the microscope. However, these objective lenses are not typically designed for oil immersion. Oil immersion lenses are designed differently and have specific characteristics that make them suitable for use with immersion oil.
Oil immersion objective lenses are used for higher magnification, typically 100X or higher. They are specially designed to work with immersion oil, which has a refractive index similar to that of glass. This oil is placed between the front lens of the objective and the glass slide, improving the numerical aperture and resolution of the microscope. This technique is particularly useful when observing very small and detailed structures, such as bacteria or fine cellular components.
Lower magnification objective lenses, like 10X and 45X, are not designed for oil immersion because they are not intended for the same level of high-resolution work that requires oil immersion. Additionally, using immersion oil with lower magnification objectives might result in distorted images, reduced image quality, and even damage to the lens.
So, while objective lenses are available in various magnifications, the use of oil immersion is specifically limited to higher magnification objectives that are designed for this purpose.
Why don't you just make all the objectives able to to be immersed in oil without damage even if they don't need the oil?
*dabs*
Short, sweet and to the point. Good clarity.
THANK YOU!!
Thank you! This helped