We Dipped Our Lens in Oil to See More Detail
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- เผยแพร่เมื่อ 12 มิ.ย. 2024
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Oil immersion is an interesting and complex microscopy tool.
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I read the title and thought you put oil on your eyes
I also make videos of microscopic beings!!!
Micros are getting a lot more clearer With more details since your last camera scope.
U say u add oil because lights going threw air... So what if it was in a vacuum...? Would you just skip the oil? And what if u didn't
You should try to find (or make) an algorithm that synthesizes/extracts sound effects from microscope footage so that you can hear what's going on in the microscopic world. Maybe you could even utilize different sensors to help out.
I love my 100x objective, It’s literally breathtaking and you can imagine my emotions when I am able to see my pond buddies in way superior details! I appreciate everyone who joins us here, thank you for supporting our channel and making this real! Only regret I have is not getting the 100x objective before publishing my book, but I promise my second book will have many photos through the 100x because I cannot get enough of these details!
-James
Your 2nd book will be a gem!
Howdy, what is the real magnification that you can get with white light (and oil and every other of your tricks). Can you get an even higher magnification by going to a shorter wavelength of light? Perhaps instead of normal colors say blue and two different wavelengths of near UV? What is the highest magnification that can be done on a living thing, and what might it take to equip you to go hunting for it? I'm a patreon and I am just so glad to be able to help you (and Hank and the others) to help make this show. Dziękuję James i Kontynuuj dobrą robotę
@@aldenconsolver3428 ahh thank you! I wish I could speak Polish but I am just living here for 4 years and that’s not enough to learn this language. 😂 The maximum magnification is somewhere around 1000x, changing the wavelength to something smaller only changes 10-20 nanometers in resolution. There are some online calculators for that if you want to get more detailed information. But it’s safe to say, 1000x is the highest magnification you’ll ever get on this channel with an optical microscope, but we may dive into the realm of electron microscopy some day then can show pictures at 50000x 😀
Would it be possible by using several frequencies of near-ultraviolet to make a a pseudo color image of a living creature (it seems to me that near UV would not immediately kill a normal cell as long as the illumination was not excessive). While working at JPL I came up with a system for ‘sharpening’ images using chi-square binning and various sobel style edge sharpeners- would this be useful in microscopy? This can also be used to reduce the effect of jitter in matching images. I also used some broadly similar techniques for analyzing ground-based light observations of Venusian clouds and all things considered it worked pretty good.. I would gladly take on doing that again if it would make for better living images. I posted a comment a little while ago then decided I needed to add some more details. Thank You
Thank you for all your dedication to the field and this channel, James! Your enthusiasm is infectious and charming, and I really enjoyed "hanging out" with you and the Microcosmos team during the live stream. 😁❤️👍
Moral of the story: Tardigrades are filled with beans
but sadly no toe-beans.
@@SaschaUncia but they do have lil claws!
One of the toughest creature in existence but it looks like it's made of gummy bears
They also have some special stuff at 4:01
@@yvc9 kleiner Gummibär
There's just something mind-blowing about watching a ciliate remain stationary and then "deciding" to move. I find it insane that something so small has the ability to compute even this.
The fact that we can astounds me even more to be honest. We're nothing but collections of cells, "deciding" to move together. And think.
It is, word used in its literal definition, awesome.
Did it decide though? Or just a programmed reaction to stimuli?
@@Bhatt_Hole Please note the quotation marks. You could argue the same thing about humans; we simply react to our input, we may just be complex enough that we _appear_ to have free will.
@@Bhatt_Hole I agree with this. So are we, but I think that consciousness is an emerging product of a being capable of processing information, retaining memories and being aware of itself to a complex enough level.
I watch this series with my son, he's currently too young to understand what is going on, but eventually I think he will enjoy this series as much as I do!
How old is he?
@@limiv5272 2, he really is just looking at shapes and sounds at this point.
Your son does have a cool dad, greet him from me :)
@@thomasrogers8239 I think most viewers of this channel come here for the fascinating shapes and calming sounds (-:
I love the channel too, but I think I'M too young to understand what's going on in this one!
Wow the detail on the Tardigrade is mind blowing, looks like you can see 'skin' detail. Would love for you to revisit some of the 'larger' animals and really dig into their surface detail
2:35 "But you should never trust an optics lesson that seems simple." As an optics enthusiast, I couldn't agree more. Well said.
Heheheheh.
Yes.
As somebody who wears glasses, the concept of "oil + lens = better" is bonkers
That's because the oil isn't pressing into your eye, ahaha
That's some insanely detailed footage, almost felt like I could reach over and pet a Ciliate :D
If you stick your hand in a pond, you’ll pet hundreds at once.
HAHA YEAH WOW OMG PET A CILIATE WOW
@@Benzene265 thank you so much for phrasing it like this, im gonna think about it next time I go to a body of water
@@Benzene265 😂
James is a very skilled microscopist!
Oil immersion lens?... FINALLY!!! I was so much curious about oil immersion when I learned them in my microbiology first semester.
Didn't you get to use them?
Oil immersion is used with the 100X lens of a microscope, it’s pretty cool.
ONLY if it is designed for oil immersion, not because it is 100x mag.
@@rickkwitkoski1976 Haven't come across a non oil immersion 100X in a long time.
@@KP3droflxp well I didn't had any offline classes last year, so didn't use them for my lab...
I have shown these videos to my old grandmother but she denies accepting the fact that they are found everywhere and is just a matter of magnifying these samples. So please can you MAKE A VLOG LIKE a video where you start from the sample collection itself to how you manage to get it in-camera? please!!
It honestly sounds like your mother is lacking in some quality that would allow her to believe you. Almost entire planets know these organisms exists, There are entire fields of scientific study and research devoted too micro-organism. To claim micro-organisms do not exist goes against the present day to day realty we find ourselves in.
Old people tend to be more stubborn and less open to new ideas. But I think with enough time you’ll convince her.
Hate people like that!
Really? How old?
With degrees in Biology, Computer Science and Education... THIS 'old person' understands a LOT more than so many younger people.
Explain to me exactly how your "smart" phone and the internet works. Betcha can't.
To claim micro-orgasims do not exist is just WILLFUL ignorance.
Just like supposed "doctors" who now say viruses aren't real.
That's OK, except that people then believe them, cause they are "doctors"!
"the risks are negligible"
Negligible, so, non-zero...
You could die from cuteness overload when looking at a tardigrade
a pathogen in the sample
th-cam.com/video/LkBmLiDPbxQ/w-d-xo.html here is the non-zero situation.
please do more of this oil capture, one long video of something happening in the microcosmos
That’s a great idea!
-James
I would love to see older topics revisited with the new objectives. Personally would like to see the hunting and eating episodes. Would be interested how what it looks like when that vampire cell pierced and sucked the cytoplasm out of those silica shelled tubes. Sorry forgot the names of both.
You are right! Vampyrella would look gorgeous through this lens! I’ll keep my eyes open!
-James
!!!
@@JamsGerms YAY!
Oil makes lenses see clearer
1) Wait for it to Vision
2) Cover yourself in oil
3) Congrats, you have unlocked 1080p in real ljfe
As a hematology lead in a medical lab, I use oil immersion every day. Amazing the level of detail you can get with it!
Hank: The cave is metaphorical.
Plato: Did somebody says Cave?
Ok, imagine a fire...
But you can't see the fire, so only imagine the shadows.
Cave Johnson: Did somebody say Aperture?
@@BeardslapRadio Kind of...😜
Hmm... It sounds... Allegorical.
*Forcefully eats hemlock for denying the matrix exists.*
God, I adore you guys and this channel. You really do incredible work and your fans love you for it.
This is one of the most amazing things I have ever seen. Completely in awe of microscopic life
This channel ease the pain caused by the uncertainty of being. I really appreciate!
Some of the most coveted (and expensive) triplet apochromatic refractor telescopes feature an "oil spaced" triplet lens, for similar reasons as illustrated in this video, to soothe the glass-air-glass effects on refraction an air spaced triplet might have. However, don't be fooled, the views do not improve to the magnitude that they do in this case with microscopes. That's because ground-based telescopes have to look through Earth's atmosphere, which is often the biggest limiting factor into what details you can see and how high you can push the magnification before the image breaks down. The amount of turbulence in the atmosphere varies by night, so you never know where the limits are until you go out and try out different eyepieces.
It's amazing to me that there are people that don't understand just how much the atmosphere can distort telescope images. When I got LASIK a few months ago, one of the first things I noticed while watching the night sky was that I could actually see ripples moving across the surface of the moon as the air cooled from an unusually hot day. It was amazing.
@@GuyNamedSean That's awesome! Congrats on your successful procedure. When doing outreach, (chilling in public with my scope to share the view, unfortunately haven't done much of that since the whole pandemic) I've always told others when viewing the moon that the atmospheric turbulence resembles the "mirage" effect that happens in the desert or viewing over a hot car hood. Really helps the public understand why we must send telescopes to space to get our deepest and most detailed observations, and also dispel the misconception that we send telescopes to orbit to get closer to the stars.
Can I put in an order for a “Microcosm of the Gut.” ? I think it would be cool to see some bacterium and learn about the human gut with the new upgraded gear.
This was so useful. I studied physics at university (college) so we didn’t touch on optical microscopes much (I actually did a PhD in electron microscopy). I recently bought a microscope after having my interest aroused (!) after restoring a friends old microscope (I did, of course study optics). The microscope came with oil, but this seemed to go against everything i had ever been taught about microscopes. Now I see that my 100x objective is marked “oil”. Thank-you can’t wait to try it!
As Hank explained, light refracts when it exits a substance and enters medium of a different density. Oil on the coverslip has a similar density to glass and cuts out that "middleman" interruption of "thin air" that so distorts a highly magnified image.
That Keratella is super-interesting and I want to know more! Can you do an episode on them? Even Wikipedia's article on them is barren.
That little Keratella creature is incredible, amazing how much life is going on all around us
I have to say these are some of the most detailed microscopic images I've ever seen. Well done.
It's always so wonderful to hear Hank get excited about science! What a fascinating way to manipulate light for a better image. I had no idea such a thing was even possible - but then, my old (practically ancient) high school biology classroom was likely provided with the least expensive, least sophisticated equipment - the bare minimum, so that when something got broken (as inevitably it would) it would not be too expensive to replace.
These videos, these images, are such a feast for the eyes, with their vibrant colors. And with the awesome music and lovely narration, it just becomes something that I look forward to every single week.
Thank YOU so much, for creating this journey, to share with us!
How much more incredible can this be? Wowed once again!
Great channel Hank, I could barely tell it was you at the start. You sound much more calm and measured than on some of your other channels and it feels as though it lends a more thoughtful affect to the content of your commentary.
Loved the video, interesting subject and very striking images.
More about the Keratella Plz 3:16 that thing looks like it's from another dimension
I love the interplay with imagery, instruction, and wonder. Thank you guys so much!
I was just looking at the community post and looking forward to this.
This was part of my high school biology class but mostly from books. This way makes it so much more wonderful. It was nice to see all the tiny creatures alive. Unicellular life is hard to conceive when all you have are basic illustrations. Here you can see all the tiny processes inside. It makes them much more real
The clarity and level of complexity visible at 1000x is amazing!!! Nature has taken every opportunity to assemble structures and put things together in a utilitarian way. I bet this would go down to the molecular level if we could only see that far.
Absolutely gorgeous footage, James! I am so excited for you having new microscope updates and the results have already been so incredible! 😍
Words cannot describe the beauty of this footage.. It's so good that it brought tears to my eyes! This video, with Hank's calm narration, makes it simply incredible!
Hank reading Journey to the Microcosmos means everything is again right with the world.
The whole team is superb but the narration is magical.
Never stop making videos, they are all tiny treasures. 🥺
Love it when you explain the equipment.
This is astonishing footage, the improvements over the course of your channel from the beginning to now are on an evolutionary scale.
Wow :O Thank you so much for sharing this incredible footage!
This channel is not only super interesting, it really calms me down when im anxious.
Thank you hank and the gang
@Journey to the Microcosmos I believed I subscribed sometime two years ago I think. But haven't watched every episode, I admit. The reason I joined was because these video's are just so darn fascinating! With now my favorite; hands down being about the Lacrymaria! What a truly bizarre, yet utterly fascinating organism! Great job, and content, I hope you keep up the fantastic work! Best of luck in your future endeavors!
Amazing picture quality! So much detail I love it!
Incredible narration and footage. Ty very much. Gives an amazing perspective. Life is amazing.
Amazing as always! And fantastic speaker voice!
I absolutely loved this video, such excellent quality footage captured with genuine love and intrigue
Imagine what Antonie van Leeuwenhoek would think if he saw these images?
I really appreciate your explanations of the technical aspects of your images.
Every journey is a masterpiece!
I'm sorry, I just love your images, your music, your stories and lessons, your writers and narrators, your videos and your whole channel. I believe it is one of the most beautiful things I've encountered in my life on youtube. thank you :)
Thank you. For opening my eyes so deep it has awoken my inner child's curiosity! Please keep up this inspiring exploration!
This channel is so great. I wonderful blend of educational and soothing. The music for this episode is especially good!
I have recently discovered this channel and I'm binge-watching all of the episodes. Still shocked how cool and high-quality content here.
Narator's voice is very charming, cozy and calming.
This was so fascinating. I freaking love this channel and how much I learn even beyond just facts about various microbes
Thanks for the stellar details of the microcosms.
This is a beautiful video showing life under an oil immersion lens. Thank you for sharing
This video will be shown in so many microscopy classes! Great job explaining this stuff!
Stunning resolution - thank you :-)
I feel like I’ve just had a science/technology lesson and a philosophy lesson all in less than ten minutes. I’ve been watching this channel for a while and is so informative. Thank you Hank for your wonderful voice that makes this channel such a joy to listen to.
That is the way I love this show! Absolutely wonderfully relaxing. So calm.
Im just as excited as you. I cant wait for more and more
Absolutely gorgeous imagery.
Wow, 2021 we can see this absolutely incredible footage from something so miniscule with such detail, it really is mind-blowing when you realise what you're seeing so vividly
Outstanding explanation. Thank you so much for all of your work.
As a newbie in the microscopy hobby, these in-depth and high-quality videos about the fundamentals are incredibly valuable. Please keep up the amazing work!
That last clip was awesome. Watching that guy pump out the water.
The videos are amazing, but your narration is excellent also. Kudos to you for it.
Using optical magnification to look at nature is awesome, whether you’re looking at Rotifers through a 630x microscope or an Alligator through 8x binoculars.
Those images are incredible
It is fascinating how a new degree of microscopy opens up a brand new level of the universe.
The music makes the detailed views even more magical.
Underrated channel. Needs more subs.
I have to date never paused a TH-cam video as many times as I just did watching this episode. Truly amazing to behold that level of microscopic detail. ✌🏼🤟🏼🖖🏼👏🏻👏🏻👏🏻👏🏻👏🏻
Some of the most amazing content on TH-cam!!! Keep it up :)
Very well explained. Very interesting. 👍🏻👍🏻
I'm always baffled at the amazing images you guys have
. COOL. I _KNEW_ THERE WAS A METHOD Y'ALL WERE USING
to get that increased detail. I had more fun learning about it
from this video than I would have looking it up somewhere.
. GREAT STUFF!!!
these images are spectacular!
This was really interesting! Thank-you for explaining the differences. I didn't know that oil is used on some occasions. Now I understand and know why. You guys rock! 😎🤘
I am a graduate of microbiology and still it is so peaceful to watch it even after repeating same things many time. More power to you guys
I learned something today, thank you.
This is just amazing. Simple science but wow. thank you.
Great job! Watching from Brazil =) I like your channel
I absolutely love this channel
Ooo yes just in time for lunch!
Some pretty amazing images. Being able to clearly see all the cilia going nuts is... kinda nuts. :D
Stroke of brilliance!! Awesome !!!
Wow, wish you guys thought at my school.....very interesting. Thanks.
I never knew about this technique! It's amazing!
James, our master of microscopes, is full of neat secrets!
This was such an amazing episode
I love you guys. Thank you.
I don't feel so bad about eating fries while my kid shows me what she found in the ditch water now.
lool
Its so impressive to see the change with and without oil. Great job guys!
such amazing footage its a shame more havent seen it
beautiful detail
Amazing images
That last monologue was beautiful