How to enhance fluorescence intensity in imageJ | Confocal images not showing fluorescence in imageJ

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  • เผยแพร่เมื่อ 1 ต.ค. 2024

ความคิดเห็น • 11

  • @simonyacoub8262
    @simonyacoub8262 2 ปีที่แล้ว +3

    Thank you! This is exactly what I needed for lab!!!!! life saver

    • @AliceritaE
      @AliceritaE  2 ปีที่แล้ว

      You're very much welcome.

  • @sunnetinternationalbusines9910
    @sunnetinternationalbusines9910 27 วันที่ผ่านมา +1

    vielen Danke

    • @AliceritaE
      @AliceritaE  27 วันที่ผ่านมา

      Gerne!

  • @turkdogt6251
    @turkdogt6251 ปีที่แล้ว +1

    Could you post a video on how to properly threshold to separate a cluster of cells to individual cells

    • @AliceritaE
      @AliceritaE  ปีที่แล้ว +1

      Hello, I will work on that and hopefully upload the video by the weekend.

  • @snehasishnag1324
    @snehasishnag1324 ปีที่แล้ว +1

    Can i use these adjustments in imageJ for publications?

    • @AliceritaE
      @AliceritaE  ปีที่แล้ว

      Yes, but you have to add the adjustment to your materials and method section. E.g microscope images were further adjusted I'm imageJ to increase the intensity signal.
      Although, the image I showed in this tutorial is bright under the microscope, when I opened it in imageJ, it looks darker. The only edit I normally apply would be to use the RESET on the contract/brightness window. Then I get a brighter image and I don't have to add that yo material and methods

  • @b.9744
    @b.9744 2 ปีที่แล้ว +1

    Thank you so much!

    • @AliceritaE
      @AliceritaE  2 ปีที่แล้ว

      You're welcome 😊