What is PCR?

Why did an adolescent boy start growing breasts? And how did PCR help scientists solve the mystery? This video explains what PCR is and how it works - all in the context of how a team of scientists diagnosed an adolescent boy with an XX + SRY genotype.
The research story from this video is based on this paper:
M. S. Kim, P. H. Hwang, D.-Y. Lee, A 46,XX Male Adolescent Presenting with a Chief Complaint of Gynecomastia. Pediatrics & Neonatology 56, 357-359 (2015). doi.org/10.101...
In addition to using PCR, the scientists used Gel Electrophoresis to determine the patient's genotype - here is a video about Gel Electrophoresis:
What is Gel Electrophoresis? • What is Gel Electropho...
Cartoon Illustrations by Michelle Lotker
Image of Kary Mullis by Dona Mapston - CC BY-SA 3.0
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[@shivanipandita8921]

Mam, your work is unparalleled. Thank you so much for putting so much effort in making these marvellous content and sharing with us :)

[@YouTooBio]

Thank you! 🥰

[@Sadiq126]

Thank you, mam you are genius. The way you explain topic is very understandable and the viewer gets what you want him/her to know.

[@phila2938]

Great video and context! Just doing PCR with my A Level class. Thanks.

[@user-pn4re8oy6b]

please do more videos on laboratory equipment.
it is very useful for the people to understand them one by one

[@hadeelb7678]

Oh Gosh! I love you!! The information you add to your videos are amazing! I am glad I discovered your channel!

[@YouTooBio]

Thanks! 💜

[@bernardojunqueira2392]

This video was perfect for a complete noobie in PCR. Thanks. It helped a lot.

[@goroseigandhi8811]

Thankyou very much

[@betterdays2011]

Thank you for your video!! Your example helps me understand so much better in detail!
I have a question on SRY gene replication using PCR. How will DNA polymerase know when to stop and only replicate SRY+ gene?

[@YouTooBio]

Good question! The answer has to do with primers, but it's hard to explain. I suggest watching this excellent animation of PCR to see why only the DNA of interest gets amplified th-cam.com/video/JRAA4C2OPwg/w-d-xo.html

[@snarlymacsnarls488]

Wow, thank you so much for this video, I really wanted to know about PCR :)

[@islamsaleh4697]

very informative. Thank you

[@jta831]

What would the optimal temperatures usually be for DNA denaturation, primers binding and DNA polymerase ?

[@alimahrez7122]

Wow amazing keep going please

[@rodolfodelagarzaramirezsan1414]

Loved the video

[@YouTooBio]

Thanks!

[@mariaagustin5368]

Super clear

[@JunZhou-mn8ir]

the lesson is fun with knowledge.

[@acexspade9067]

How do they know where to set the two primers in order to get the copys of sry? If they knew were sry was already why did they have to test for its presence?

[@David_Hogue]

I finally understand why all those fb comments in 2020 didn't add up

[@malinduanjana3071]

Wow! Nice explaination

[@adnanramazanpour6438]

Excuse me what is the illness's name you refered to at the begining?that sry +xx

[@craigharbin1513]

Great practical info. Easy to understand. However, the final gender comment can confuse vulnerable minds. Keep up the good science without the social opinion.

[@islamsaleh4697]

very well said

[@legaspy]

💖❤️😍